pHrodo™ Green E. coli BioParticles™ Conjugate for Phagocytosis

pHrodo™ Green <i>E. coli</i> BioParticles™ Conjugate for Phagocytosis

Citations & References (19)

Invitrogen™

pHrodo™ Green E. coli BioParticles™ Conjugate for Phagocytosis

製品番号（カタログ番号）	数量
P35366	5 x 2 mg

製品番号（カタログ番号） P35366

価格（JPY）

63,500

お問い合わせください ›

5 x 2 mg

新しいpH感受性の高いpHrodo™ Green色素コンジュゲートは、当社のpHrodo™ Red色素コンジュゲートと同様に、他のあらゆるファゴサイトーシスアッセイよりも迅速かつ正確な結果を提供します。pHrodo™ Green複合体は、中性pHでは細胞外で非蛍光性ですが、ファゴソームなどの酸性pHでは明るい緑色の蛍光を発します。より迅速な染色とより正確な結果—洗浄ステップやクエンチャー色素は不要です。

•食作用およびエンドサイトーシスの特異的検出
• 感度の高い実験では、信号の変動が減少し、タイミングが改善されます
• 96ウェルプレートの各ウェルで100 µLを使用すると、100回の試験を実行するのに十分です
• 赤色蛍光タンパク質、TMRM、NucBlue™ Hoechst、CellROX™ディープレッドなどの他の適合する色素と多重化する

新規のpHrodo™緑色色素の蛍光は、pHが中性から酸性に低下するにつれて劇的に増加するため、食作用と、薬物や環境因子によるその調節を研究するための最適なツールになります。細胞外の蛍光がないため、洗浄ステップやクエンチャー色素が不要です。

イメージング、ハイコンテントスクリーニング、ハイスループットスクリーニング、およびフローの各用途で、すぐに使用できるpHrodo™緑色大腸菌 BioParticles™コンジュゲートを使用します。pHrodo™緑色色素は、ザイモサンBioParticles™、黄色ブドウ球菌BioParticles™、またはデキストラン10,000 MWとしても利用できます。抗体コンジュゲートなどの他のコンジュゲートを作成するには、pHrodo™緑色STPエステルまたはpHrodo™緑色マレイミドを使用します。

本製品は研究用試薬です。ヒトまたは動物の治療もしくは診断目的には使用できません。

研究用途にのみご使用ください。ヒトまたは動物の治療もしくは診断目的には使用できません。

細胞タイプMammalian

概要pHrodo™ Green E. coli BioParticles™ Conjugate for Phagocytosis

検出法蛍光

染色剤タイプpHrodo™ Green

励起／発光509⁄533

数量5 x 2 mg

出荷条件室温

種E. coli

色Green

使用対象（アプリケーション）Cell Analysis

使用対象 (装置)Attune Acoustic Focusing Cytometer, Confocal Microscope, Floid Cell Imaging System, Fluorescence Microscope, High Content Analysis Instrument

製品ラインpHrodo

製品タイプBioparticle Conjugate

Unit SizeEach

組成および保存条件

-20℃で保存し、乾燥させ、遮光します。

よくあるご質問（FAQ）

I am performing a phagocytosis assay of macrophages engulfing pHrodo-labeled bacteria. What do you recommend for fixation after the phagocytosis?

pHrodo is relatively non-fluorescent until it enters the acidic phagosome, at which point its fluorescence increases. If you fix the sample, the pHrodo will only reflect the pH of the buffer the cells are in, and not the pH of the phagosome. For this reason, we do not recommend fixing samples. If you want to see how many cells engulfed the labeled bacteria, fix the cells and then place the fixed cells in an acidic buffer for the assay.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I store reconstituted pHrodo BioParticles Conjugates for Phagocytosis and Phagocytosis Kit, for Flow Cytometry?

Yes. Once reconstituted, pHrodo BioParticles Conjugates for Phagocytosis and Phagocytosis Kit, for Flow Cytometry (Cat. Nos. P35367, P35361, P35360, P35366, P35364, P35365, A10010) can be stored at 2 - 8 degrees C for several weeks, as long as sodium azide is added to a final concentration of 2 mM. If no sodium azide is added, the cell suspension needs to be used right away or on the same day to avoid contamination. DO NOT FREEZE the resuspended pHrodo bioparticle conjugates.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How many bioparticles are provided in pHrodo Green E. coli BioParticles Conjugate for Phagocytosis (Cat. No. P35366)?

pHrodo Green E. coli BioParticles Conjugate for Phagocytosis (Cat. No. P35366) contains ~3 x 10⁸ bioparticles/mg bioparticles before conjugation. We do not count the number of bioparticles after conjugation.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Are the Invitrogen BioParticles products sterile?

While the bacteria have been attenuated with formaldehyde and alcohol desiccation, the BioParticles products are not considered sterile, and we do not recommend incubation of more than 4 hours. This applies to all of our dye-labeled (pHrodo, Alexa Fluor, etc.) and unlabeled BioParticles products.

What is the type of bond that attaches the dyes to the BioParticles probes?

We use amine-reactive dyes to covalently attach fluorescent dyes to all of our BioParticles probes such as the Escherichia coli (K-12 strain) BioParticles probes, Staphylococcus aureus (Wood strain without protein A) BioParticles, and the Zymosan A (S. cerevisiae) BioParticles probes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all pHrodo™ Green E. coli BioParticles™ Conjugate for Phagocytosis FAQs

引用および参考文献 (19)

引用および参考文献

Abstract

A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part I: BacMam Labeling and Reagents for Vesicular Structures.

Authors:Dolman NJ, Kilgore JA, Davidson MW,

Journal:

PubMed ID:23835803

'Fluorescent labeling of vesicular structures in cultured cells, particularly for live cells, can be challenging for a number of reasons. The first challenge is to identify a reagent that will be specific enough where some structures have a number of potential reagents and others very few options. The emergence of ... More

TREM2 mutations implicated in neurodegeneration impair cell surface transport and phagocytosis.

Authors:Kleinberger G, Yamanishi Y, Suárez-Calvet M, Czirr E, Lohmann E, Cuyvers E, Struyfs H, Pettkus N, Wenninger-Weinzierl A, Mazaheri F, Tahirovic S, Lleó A, Alcolea D, Fortea J, Willem M, Lammich S, Molinuevo JL, Sánchez-Valle R, Antonell A, Ramirez A, Heneka MT, Sleegers K, van der Zee J, Martin JJ, Engelborghs S, Demirtas-Tatlidede A, Zetterberg H, Van Broeckhoven C, Gurvit H, Wyss-Coray T, Hardy J, Colonna M, Haass C,

Journal:

PubMed ID:24990881

'Genetic variants in the triggering receptor expressed on myeloid cells 2 (TREM2) have been linked to Nasu-Hakola disease, Alzheimer''s disease (AD), Parkinson''s disease, amyotrophic lateral sclerosis, frontotemporal dementia (FTD), and FTD-like syndrome without bone involvement. TREM2 is an innate immune receptor preferentially expressed by microglia and is involved in inflammation ... More

P2X4 receptor regulates alcohol-induced responses in microglia.

Authors:Gofman L, Cenna JM, Potula R

Journal:

PubMed ID:25135400

'Mounting evidence indicates that alcohol-induced neuropathology may result from multicellular responses in which microglia cells play a prominent role. Purinergic receptor signaling plays a key role in regulating microglial function and, more importantly, mediates alcohol-induced effects. Our findings demonstrate that alcohol increases expression of P2X4 receptor (P2X4R), which alters the ... More

Induction of trabecular meshwork cells from induced pluripotent stem cells.

Authors:Ding QJ, Zhu W, Cook AC, Anfinson KR, Tucker BA, Kuehn MH

Journal:

PubMed ID:25298418

Loss or dysfunction of trabecular meshwork (TM) cells has been associated with the development of pathologically elevated IOP, and it is conceivable that replacement of damaged TM cells could restore function to the TM. We propose that the use of TM-like cells derived from induced pluripotent stem cells (iPSCs) created ... More

Pathogenic bacterial species associated with endodontic infection evade innate immune control by disabling neutrophils.

Authors:Matsui A, Jin JO, Johnston CD, Yamazaki H, Houri-Haddad Y, Rittling SR,

Journal:

PubMed ID:25024367

Endodontic infections, in which oral bacteria access the tooth pulp chamber, are common and do not resolve once established. To investigate the effects of these infections on the innate immune response, we established a mouse subcutaneous chamber model, where a mixture of four oral pathogens commonly associated with these infections ... More

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