Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents
Use 96- and 384-well Microplates for Fluorescence-based Assays with Quant-iT assays for optimal results
For accurate quantification and a streamlined workflow try our new Quant-iT PicoGreen ReadyPlates!
Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents
Citations & References (340)
Invitrogen™

Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents

Quant-iT PicoGreen dsDNA Assay KitおよびPicoGreen dsDNA Reagentを使用すると、ssDNA、RNA、および遊離ヌクレオチドの存在下でも、幅広いソースからdsDNAを選択的に検出できます。
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製品番号（カタログ番号）数量製品タイプパッケージングタイプ
P75811 mLQuant-iT PicoGreen dsDNA試薬1 x 1 mL Bottle
P75891 mL kitQuant-iT PicoGreen dsDNAアッセイキット1 x 1 mL Bottle
P1149610 x 100 μL kitQuant-iT PicoGreen dsDNAアッセイキット10 x 100 μL Tubes
P1149510 x 100 μLQuant-iT PicoGreen dsDNA試薬10 x 100 μL Tubes
製品番号（カタログ番号） P7581
価格（JPY）
130,200
Each
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数量:
1 mL
製品タイプ:
Quant-iT PicoGreen dsDNA試薬
パッケージングタイプ:
1 x 1 mL Bottle

Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。

Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に対応します。

PicoGreen dsDNA定量アッセイおよび試薬を使用すると、ssDNA、RNA、および遊離ヌクレオチドの存在下であっても、わずか0.25 pg/uLのdsDNAを迅速に検出できます。このアッセイには3桁にわたる直線的な定量範囲があり、配列依存性はほとんどないため、ゲノムDNA、ウイルスDNA、最小プレップDNA、PCR増幅産物などの多くのソースからのDNAを正確に測定できます。

PicoGreen dsDNA定量試薬には次の特長があります：
• UV吸光度測定に比べ、感度が大幅に向上し、貴重なサンプル量を節約できます。
•RNA存在下でのdsDNAに特異的です。
•使いやすく、サンプルに色素標準溶液を加え、5分間インキュベートした後、読み取るだけです。
•96ウェルおよび384ウェルプレートフォーマットでの使用に適しています
• ほとんどの蛍光式マイクロプレートリーダーおよび蛍光光度計に対応します。
•Picogreenの近接蛍光吸収／最大発光値は502/523 nmで、dsDNAに結合します。


アプリケーション
PicoGreen dsDNA定量試薬およびキットは、PCRベースのアッセイ、マイクロアレイサンプル、DNA損傷アッセイ、酵素活性アッセイ、ゲノムDNA定量、複合混合物中のdsDNA測定、ウイルスDNA定量に最適です。
For Research Use Only. Not for use in diagnostic procedures.
仕様
励起／発光500/525
使用対象 (装置)マイクロプレートリーダー
反応数200アッセイ（2 mLアッセイ容量）
パッケージングタイプ1 x 1 mL Bottle
製品ラインPICOGREEN、Quant-iT
製品タイプQuant-iT PicoGreen dsDNA試薬
定量範囲50 pg～2 μg
数量1 mL
出荷条件室温
検出法蛍光
Unit SizeEach

よくあるご質問（FAQ）

Why am I getting negative fluorescence values with my Qubit Assays?

Negative fluorescence is a physical impossibility. It is an artifact from software autocorrecting for background signal. This means your reader is picking up and subtracting out background light at the cost of your data. Make sure to do a buffer-only control and assess the type of signal. You may need to switch to a different plate.

I have a Quant-iT DNA Kit and want to use it for the Qubit Fluorometer. Can I?

Yes, the manual has directions for this application. You will use the 0 ng/µL lambda dsDNA HS standard to generate Standard #1. You will prepare a dilution of the 10 ng/µL lambda dsDNA HS standard to generate Standard #2. You then prepare the samples and compare them to this 2-point standard curve. The Quant-iT dsDNA BR Kit can be used in a similar manner.

What is the useful pH range for Quant-iT DNA kits?

The buffer included in the kit should assure the proper pH range, even if your DNA is at a pH outside of this range, since at least a 10-fold excess of kit buffer over sample is used in the assay.

I'm trying to quantify some DNA labeled with a fluorophore. Will this work?

PicoGreen dye and other fluorescence-based quantification reagents are not recommended for quantifying dye-conjugated nucleic acids. The attached dye molecules can interfere with either binding and/or fluorescence output of the quantification reagents.

Does DNA length have an effect on the dsDNA assays?

Strands that are roughly in the 20-mer range or shorter show a lower level of signal. For dsDNA samples that are composed of mostly short strands, the reagent may still be used, but one should use a dsDNA standard that is of comparable length as the sample.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

View all Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents FAQs

引用および参考文献 (340)

引用および参考文献
Abstract
Authors:
Journal:
PubMed ID:16517648
Rapid quantification of DNA samples extracted from buccal scrapes prior to DNA profiling.
Authors:Hopwood A, Oldroyd N, Fellows S, Ward R, Owen SA, Sullivan K
Journal:Biotechniques
PubMed ID:9232218
Simultaneous extraction from bacterioplankton of total RNA and DNA suitable for quantitative structure and function analyses.
Authors:Weinbauer MG, Fritz I, Wenderoth DF, Höfle MG
Journal:Appl Environ Microbiol
PubMed ID:11872453
The aim of this study was to develop a protocol for the simultaneous extraction from bacterioplankton of RNA and DNA suitable for quantitative molecular analysis. By using a combined mechanical and chemical extraction method, the highest RNA and DNA yield was obtained with sodium lauryl sarcosinate-phenol or DivoLab-phenol as the ... More
Conventional methods versus 16S ribosomal DNA sequencing for identification of nontuberculous mycobacteria: cost analysis.
Authors:Cook VJ, Turenne CY, Wolfe J, Pauls R, Kabani A
Journal:J Clin Microbiol
PubMed ID:12624023
The clinical profile of nontuberculous mycobacteria (NTM) has been raised by the human immunodeficiency virus and AIDS pandemic. Different laboratory techniques, often molecular based, are available to facilitate the rapid and accurate identification of NTM. The expense of these advanced techniques has been questioned. At the National Reference Center for ... More
Exo-proofreading, a versatile SNP scoring technology.
Authors:Cahill P, Bakis M, Hurley J, Kamath V, Nielsen W, Weymouth D, Dupuis J, Doucette-Stamm L, Smith DR
Journal:Genome Res
PubMed ID:12695330
We report the validation of a new assay for typing single nucleotide polymorphisms (SNPs) that takes advantage of the 3'-to-5' exonuclease proofreading activity of many DNA polymerases. The assay uses one or more primers labeled on the 3' nucleotide base, and can be implemented in a variety of formats including ... More
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