For Research Use Only. Not for use in diagnostic procedures.
仕様
アッセイRNA定量、広範囲
励起/発光644/673
使用対象 (装置)マイクロプレートリーダー
反応数1,000反応(200 μLアッセイ容量)
製品ラインQuant-iT
定量範囲20~1,000 ng
数量1,000 assays
出荷条件室温
検出法蛍光
Unit SizeEach
よくあるご質問(FAQ)
Why am I getting negative fluorescence values with my Qubit Assays?
Negative fluorescence is a physical impossibility. It is an artifact from software autocorrecting for background signal. This means your reader is picking up and subtracting out background light at the cost of your data. Make sure to do a buffer-only control and assess the type of signal. You may need to switch to a different plate.
I have a Quant-iT RNA Assay Kit and want to use it with the Qubit Fluorometer. Can I?
Yes, the manual has directions for this application.
Will these Quant-iT RNA kits work for siRNA or microRNA?
No, they are not accurate for such small fragments. The Quant-iT microRNA Assay Kit and Qubit microRNA Assay Kit are good tools for this application.
Can the Quant-iT RNA assay be used to quantitate RNA labeled with digoxigenin?
Yes, the tag does not interfere with the signal.
What are the linear ranges of the Quant-iT RNA kits?
The linear ranges are:
- Quant-iT RiboGreen RNA Assay: 1 ng/mL to 1 µg/mL
- Quant-iT RNA BR Assay: 20-1,000 ng
- Quant-iT RNA HS Assay: 5-100 ng
[Possible Involvement of Genes Related to Lysosomal Storage Disorders in the Pathogenesis of Parkinson's Disease].
Authors:Rudenok MM, Alieva AK, Nikolaev MA, Kolacheva AA, Ugryumov MV, Pchelina SN, Slominsky PA, Shadrina MI
Journal:
PubMed ID:30895950
Parkinson's disease (PD) characterized with slow continuous degeneration of dopaminergic neurons in the substantia nigra is one of the most common neurodegenerative diseases, but its etiology and pathogenesis are not fully understood. The pathogenesis of PD involves the impairment of lysosomal autophagy, which also contributes to lysosomal storage disorders (LSDs). ... More
Expression analysis of genes involved in mitochondrial biogenesis in mice with MPTP-induced model of Parkinson's disease.
Authors:Rudenok MM, Alieva AK, Starovatykh JS, Nesterov MS, Stanishevskaya VA, Kolacheva AA, Ugryumov MV, Slominsky PA, Shadrina MI
Journal:Mol Genet Metab Rep
PubMed ID:32280590
'The mitochondrion is an extremely important organelle that performs various functions in the cell: e.g. energy production, regulation of respiration processes and maintenance of calcium homeostasis. Disruption of the biogenesis and functioning of this organelle can lead to cell damage and cell death. Mitochondrial dysfunction has been shown to possibly ... More
PASSPORT-seq: A Novel High-Throughput Bioassay to Functionally Test Polymorphisms in Micro-RNA Target Sites.
Authors:Ipe J, Collins KS, Hao Y, Gao H, Bhatia P, Gaedigk A, Liu Y, Skaar TC
Journal:Front Genet
PubMed ID:29963077
Next-generation sequencing (NGS) studies have identified large numbers of genetic variants that are predicted to alter miRNA-mRNA interactions. We developed a novel high-throughput bioassay, PASSPORT-seq, that can functionally test in parallel 100s of these variants in miRNA binding sites (mirSNPs). The results are highly reproducible across both technical and biological ... More
RNA editing blood biomarkers for predicting mood alterations in HCV patients.
Authors:Salvetat N, Van der Laan S, Vire B, Chimienti F, Cleophax S, Bronowicki JP, Doffoel M, Bourlière M, Schwan R, Lang JP, Pujol JF, Weissmann D
Journal:J Neurovirol
PubMed ID:31332697
Treatment-emergent depression is a common complication in patients with chronic hepatitis C virus (HCV) infection undergoing antiviral combination therapy with IFN-a and ribavirin. It has recently been shown that changes in A-to-I RNA editing rates are associated with various pathologies such as inflammatory disorders, depression and suicide. Interestingly, IFN-a induces ... More
Characterization of mast cell-derived rRNA-containing microvesicles and their inflammatory impact on endothelial cells.
Authors:Elsemüller AK, Tomalla V, Gärtner U, Troidl K, Jeratsch S, Graumann J, Baal N, Hackstein H, Lasch M, Deindl E, Preissner KT, Fischer S
Journal:FASEB J
PubMed ID:30702929
Tissue-resident mast cells (MCs) are well known for their role in inflammatory responses and allergic and anaphylactic reactions, but they also contribute to processes of arterial remodeling. Although ribosomes and cytosolic RNAs are located around secretory granules in mature MCs, their functional role in MC responses remains unexplored. Previous studies ... More