Qdot™ 655 Biotin Conjugate Kit
Qdot™ 655 Biotin Conjugate Kit
Citations & References (4)
Invitrogen™

Qdot™ 655 Biotin Conjugate Kit

ビオチン標識Qdot™ 655ナノ結晶は、ストレプトアビジンプローブの検出や、ストレプトアビジン標識分子との、またはストレプトアビジンブリッジを使用した他のビオチン化分子との非共有結合コンジュゲートの作成に使用できます。本製品は2µM溶液の250µLとして提供され、30 mLのQdot™インキュベーション緩衝液を含みます。詳細を見る
テクニカルサポートオーダーサポート
製品番号（カタログ番号）数量
Q10321MP1 kit
製品番号（カタログ番号） Q10321MP
価格（JPY）
95,800
온라인 행사
Ends: 27-Mar-2026
159,700
割引額 63,900 (40%)
Each
お問い合わせください ›
数量:
1 kit
ビオチン標識Qdot™ 655ナノ結晶は、ストレプトアビジンプローブの検出や、ストレプトアビジン標識分子との、またはストレプトアビジンブリッジを使用した他のビオチン化分子との非共有結合コンジュゲートの作成に使用できます。本製品は2µM溶液の250µLとして提供され、30 mLのQdot™インキュベーション緩衝液を含みます。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
製品タイプBiotin Qdot Conjugation Kit
数量1 kit
出荷条件室温
製品ラインQdot
Unit SizeEach
組成および保存条件
冷蔵庫（2～8℃）に保存。

よくあるご質問（FAQ）

What is the best way to remove white precipitate from my ITK Qdot nanocrystals?

Spinning your ITK Qdot nanocrystals at approximately 3,000 rpm for 3-5 minutes should remove the white precipitate from the supernatant. Use the supernatant immediately.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I see a white precipitate in my ITK Qdot nanocrystals; should I be concerned?

The precipitate in the organic ITK Qdot nanocrystals occurs with some frequency. The ITK Qdot nanocrystals sometimes include impurities that show as a white precipitate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why do my Qdot nanocrystals appear to be blinking?

Blinking is an inherent property of quantum dots; in fact, all single-luminescent molecules blink, including organic dyes. The brightness and photostability of Qdot nanocrystals makes the blinking more visibly apparent. Under higher energy excitation, Qdot nanocrystals blink even faster.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

My Qdot nanocrystals were brightly fluorescent before I mounted my samples; now I'm seeing a loss of fluorescence. Why is this happening?

Appropriate mounting media selection is very important to retain the fluorescence of Qdot nanocrystals. In our studies, Qdot nanocrystals work best with the following mountants:

HistoMount medium (Cat No. 00-8030); best for long term archiving
Cytoseal 60 Mountant
Clarion Mountant
Most polyvinyl alcohol-based mountants (limited storage time, less than weeks)
Water-based mountants (limited storage time, less than week)
Up to 50% glycerol (limited storage time, less than week)
Note: We do not recommend using ProLong mounting media with Qdot nanocrystals as it will quench their fluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why can't I freeze my Qdot nanocrystal solution?

Freezing will cause the product to aggregate. The Qdot nanocrystals cannot be dispersed into solution after aggregation.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Qdot™ 655 Biotin Conjugate Kit FAQs

引用および参考文献 (4)

引用および参考文献
Abstract
Separation of bioconjugated quantum dots using capillary electrophoresis.
Authors:Vicente G, Colón LA,
Journal:Anal Chem
PubMed ID:18278946
'Capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection was used to separate different bioconjugated CdSe/ZnS quantum dots (QDs). The QD nanocrystals studied were conjugated to the biomolecules streptavidin, biotin, and immunoglobulin G. The bioconjugated QDs showed different electrophoretic mobilities, which appear to depend upon the biomolecule that is attached to ... More
Quantum dots as efficient energy acceptors in a time-resolved fluoroimmunoassay.
Authors:Hildebrandt N, Charbonnière LJ, Beck M, Ziessel RF, Löhmannsröben HG
Journal:Angew Chem Int Ed Engl
PubMed ID:16247812
Lanthanides to quantum dots resonance energy transfer in time-resolved fluoro-immunoassays and luminescence microscopy.
Authors:Charbonnière LJ, Hildebrandt N, Ziessel RF, Löhmannsröben HG,
Journal:J Am Chem Soc
PubMed ID:17002375
A time-resolved fluoro-immunoassay (TR-FIA) format is presented based on resonance energy transfer from visible emitting lanthanide complexes of europium and terbium, as energy donors, to semiconductor CdSe/ZnS core/shell nanocrystals (quantum dots, QD), as energy acceptors. The spatial proximity of the donor-acceptor pairs is obtained through the biological recognition process of ... More
Single particle quantum dot imaging achieves ultrasensitive detection capabilities for Western immunoblot analysis.
Authors:Scholl B, Liu HY, Long BR, McCarty OJ, O'Hare T, Druker BJ, Vu TQ,
Journal:ACS Nano
PubMed ID:19453141
Substantially improved detection methods are needed to detect fractionated protein samples present at trace concentrations in complex, heterogeneous tissue and biofluid samples. Here we describe a modification of traditional Western immunoblotting using a technique to count quantum-dot-tagged proteins on optically transparent PVDF membranes. Counts of quantum-dot-tagged proteins on immunoblots achieved ... More