Qtracker™ 655 Vascular Labels

Citations & References (30)

Invitrogen™

Qtracker™ 655 Vascular Labels

Qtracker™非標的量子ドットは、小動物in vivoイメージング（SAIVI）技術を用いた血管構造の研究のために、マウスの尾静脈に注入するように設計されています。これらのナノ結晶は、組織への浸透性を高めるためにレッドにシフトした発光を伴う強い蛍光を示し、非特異的な相互作用を最小限に抑え、組織による免疫応答を低減するために特別に開発されたPEG表面コーティングを有しています詳細を見る

製品番号（カタログ番号）	数量
Q21021MP	200 μL

製品番号（カタログ番号） Q21021MP

価格（JPY）

163,000

お問い合わせください ›

200 μL

Qtracker™非標的量子ドットは、小動物in vivoイメージング（SAIVI）技術を用いた血管構造の研究のために、マウスの尾静脈に注入するように設計されています。これらのナノ結晶は、組織への浸透性を高めるためにレッドにシフトした発光を伴う強い蛍光を示し、非特異的な相互作用を最小限に抑え、組織による免疫応答を低減するために特別に開発されたPEG表面コーティングを有しています。PEG表面コーティングには反応官能基が含まれていないため、には反応官能基が含まれていないため、QTracker™非標的量子ドットはより長く循環した状態で保持され、1回の注入で最大3時間、または追加の注入でより長い時間イメージングできます。

異なる蛍光スペクトルや長いトラッキングが必要ですか？その他の哺乳類細胞トラッキング製品をご覧ください。

主な特性：

•Qtracker™ 655ラベルは、Ex/Em（405～615/655）nmを有しています
• 小動物in vivoイメージングのために設計されています
• 尾静脈注射を介して導入され、注入後3時間までのためにイメージングすることができます
• 4色で利用可能—565 nm、655 nm、705 nm、または800 nm発光で

SAIVIとQdot™ ナノ結晶のアプリケーションの詳細をご覧ください。

研究用途専用です。ヒトまたは動物の治療もしくは診断用には使用できません。

研究用にのみ使用できます。診断用には使用いただけません。

濃度2 μM

染色剤タイプQdotナノ結晶

製品ラインQTRACKER、Qdot

数量200 μL

試薬タイプ血管イメージング試薬

出荷条件室温

技術In Vivoイメージング

製品タイプ標識

Unit SizeEach

組成および保存条件

200 μl Qtracker™非標的量子ドット（μ50 mmホウ酸バッファー中の 2 M溶液、pH 8.3）を含みます。2–6°℃で保管してください。冷凍不可。6カ月以上安定。

よくあるご質問（FAQ）

When I label with Qtracker cell labeling reagents, I get a punctate label pattern. How do I make it more uniform?

Qtracker cell labeling reagents are taken up by the cell through endocytosis and sequestered in endosomes. This gives the label a punctate or vesicular appearance. This is normal. There is nothing that can be done to make it appear uniform throughout the cytoplasm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long after injection should I start imaging my mouse?

The imaging time course varies with the nature of the injected agent. Vascular tracers are visible in the blood vessels immediately after injection and may be imaged for several hours. Conjugated whole IgG antibodies reach their targets within a few hours of injection and may be imaged for several days.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What happens to unconjugated dye in the mouse after small animal in vivo imaging?

The dye will be eliminated via the bladder. The bladder signal is detectable within ~3 minutes of IV injection of the dye and clearance with ~30 minutes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What amount of Qtracker non-targeted reagent should I inject to image vasculature?

A recommended starting dosage is 25-50 µL of Qtracker reagent diluted to the desired injection volume with PBS or normal saline. Qtracker reagent should be diluted immediately prior to injection. DO NOT STORE DILUTED. You will need to determine the optimal dosage for your experimental models.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to track my cells with a nucleic acid stain, like DAPI or Hoechst dye. Do you recommend this?

This is not recommended. When these stains bind to DNA and RNA, they may affect the normal function of the nucleic acids, disrupting transcription, as well as replication. Other reagents, such as CellTracker dyes or Qtracker reagents are more optimized for tracking without disrupting normal activity. If a nuclear label is still desired, though, and the cells are mammalian and non-hematopoietic, CellLight nuclear reagents can transiently transfect cells to express GFP or RFP on a nuclear-expressing protein for up to several days without affecting function.

Find additional tips, troubleshooting help, and resources within our Cell Tracing and Tracking Support Center.

View all Qtracker™ 655 Vascular Labels FAQs

引用および参考文献 (30)

引用および参考文献

Abstract

Intracellular fluid flow in rapidly moving cells.

Authors:Keren K, Yam PT, Kinkhabwala A, Mogilner A, Theriot JA,

Journal:Nat Cell Biol

PubMed ID:19767741

'Cytosolic fluid dynamics have been implicated in cell motility because of the hydrodynamic forces they induce and because of their influence on transport of components of the actin machinery to the leading edge. To investigate the existence and the direction of fluid flow in rapidly moving cells, we introduced inert ... More

Circulating Bmp10 acts through endothelial Alk1 to mediate flow-dependent arterial quiescence.

Authors:Laux DW, Young S, Donovan JP, Mansfield CJ, Upton PD, Roman BL,

Journal:

PubMed ID:23863480

'Blood flow plays crucial roles in vascular development, remodeling and homeostasis, but the molecular pathways required for transducing flow signals are not well understood. In zebrafish embryos, arterial expression of activin receptor-like kinase 1 (alk1), which encodes a TGFß family type I receptor, is dependent on blood flow, and loss ... More

In vivo diffusion analysis with quantum dots and dextrans predicts the width of brain extracellular space.

Authors:Thorne RG, Nicholson C

Journal:Proc Natl Acad Sci U S A

PubMed ID:16567637

'Diffusion within the extracellular space (ECS) of the brain is necessary for chemical signaling and for neurons and glia to access nutrients and therapeutics; however, the width of the ECS in living tissue remains unknown. We used integrative optical imaging to show that dextrans and water-soluble quantum dots with Stokes-Einstein ... More

Variables influencing interactions of untargeted quantum dot nanoparticles with skin cells and identification of biochemical modulators.

Authors:Ryman-Rasmussen JP, Riviere JE, Monteiro-Riviere NA

Journal:Nano Lett

PubMed ID:17408303

Skin cells (NHEK) take up untargeted quantum dots (QD) with surface polyethylene glycol (PEG), amines, and carboxylic acids, but the mechanisms are unknown. Time courses of QD-NHEK interactions were determined and effects of QD surface coating, temperature, culture medium supplements and inhibitors of the cell cycle and endocytosis identified. The ... More

Intravital FLIM-FRET imaging reveals dasatinib-induced spatial control of src in pancreatic cancer.

Authors:Nobis M, McGhee EJ, Morton JP, Schwarz JP, Karim SA, Quinn J, Edward M, Campbell AD, McGarry LC, Evans TR, Brunton VG, Frame MC, Carragher NO, Wang Y, Sansom OJ, Timpson P, Anderson KI,

Journal:

PubMed ID:23749641

Cancer invasion and metastasis occur in a complex three-dimensional (3D) environment, with reciprocal feedback from the surrounding host tissue and vasculature-governing behavior. In this study, we used a novel intravital method that revealed spatiotemporal regulation of Src activity in response to the anti-invasive Src inhibitor dasatinib. A fluorescence lifetime imaging ... More

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