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Qubit miRNA (microRNA) Assay Kitは、小型RNA（約20のヌクレオチドまたは塩基対）の正確かつ精密な定量を実現します。これらのmiRNA定量キットにより、microRNAやsiRNA、一本鎖および二本鎖RNAを含むあらゆる種類の小型RNAを迅速に検出することができます。このアッセイは、rRNAや大型mRNAよりも小型RNAに対して高い選択性を持ち、塩類、溶媒、界面活性剤などのコンタミネーションにある程度の耐性があります。
Qubit miRNA Assay KitをQubit蛍光光度計と併用することで、microRNAサンプルの定量を正確かつ選択的に行うことができます。このアッセイは rRNAや大きなmRNA（>1,000 bp）よりも microRNAに対して高い選択性を示します。このアッセイキットはサンプル量に応じて、0.025～150 ng/μLの初期miRNAサンプル濃度に対して正確であるように設計されており、0.5～150 ngの検出範囲を実現します。
Qubit microRNA Assay Kitは、濃縮アッセイ試薬、希釈バッファー、および希釈済みmiRNA標準を提供します。希釈バッファーでアッセイ試薬を希釈し、サンプル（1 µL～20 µL）を添加した後、Qubit Fluorometerで濃度を読み取ります。
注意：
Qubit microRNA Assay Kitは、濃縮アッセイ試薬、希釈バッファー、および希釈済みmiRNA標準を提供します。希釈バッファーでアッセイ試薬を希釈し、サンプル（1 µL～20 µL）を添加した後、Qubit Fluorometerで濃度を読み取ります。
注意：
- 薄壁、透明ウェル、0.5-mL PCRチューブ（カタログ番号Q32856）をQubit 4 Fluorometerに使用、8 x 200 μLのチューブストリップ（カタログ番号Q33252）を使用してください。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
検出法蛍光
励起／発光498/518
使用対象（アプリケーション）microRNA定量
使用対象 (装置)Qubit Fluorometer
反応数500 Reactions
製品ラインQubit
製品タイプmicroRNA Assay Kit
数量500 assays
出荷条件室温
Unit SizeEach
よくあるご質問（FAQ）
How does the accuracy and sensitivity of the Qubit quantitation assays using the Qubit fluorometer compare to a microplate reader?
Can the Qubit kits give an indication of sample quality in nucleic acid samples?
Can I use the Quant-iT and Qubit Kits with other fluorometers?
引用および参考文献 (7)
引用および参考文献
Abstract
Detection of microRNAs in DNA Extractions for Forensic Biological Source Identification.
Journal:J Forensic Sci
PubMed ID:31107550
'Molecular-based approaches for biological source identification are of great interest in the forensic community because of a lack of sensitivity and specificity in current methods. MicroRNAs (miRNAs) have been considered due to their robust nature and tissue specificity; however, analysis requires a separate RNA extraction, requiring an additional step in
Defining quantification methods and optimizing protocols for microarray hybridization of circulating microRNAs.
Journal:Sci Rep
PubMed ID:28798363
MicroRNAs (miRNAs) have emerged as promising biomarkers of disease. Their potential use in clinical practice requires standardized protocols with very low miRNA concentrations, particularly in plasma samples. Here we tested the most appropriate method for miRNA quantification and validated the performance of a hybridization platform using lower amounts of starting
Circulating miRNAs, isomiRs and small RNA clusters in human plasma and breast milk.
Journal:PLoS One
PubMed ID:29505615
Circulating small RNAs, including miRNAs but also isomiRs and other RNA species, have the potential to be used as non-invasive biomarkers for communicable and non-communicable diseases. This study aims to characterize and compare small RNA profiles in human biofluids. For this purpose, RNA was extracted from plasma and breast milk
Total RNA extraction from tissues for microRNA and target gene expression analysis: not all kits are created equal.
Journal:BMC Biotechnol
PubMed ID:29548320
microRNAs (miRNAs) are short non-coding RNAs that fine-tune gene expression. The aberrant expression of miRNAs is associated with many diseases and they have both therapeutic and biomarker potential. However, our understanding of their usefulness is dependent on the tools we have to study them. Previous studies have identified the need
Differences in microRNA expression between melanoma and healthy adjacent skin.
Journal:BMC Dermatol
PubMed ID:30611259
The tumor microenvironment is composed of cancer-associated fibroblasts, tumor-associated macrophages, endothelial cells, immune cells, signaling molecules and extracellular matrix structures, which closelycommunicate with the tumor via multiple mechanisms. MicroRNAs are paracrine regulators that provide a direct interaction between the microenvironment and cancer cells. In the presentstudy, we aimed to identify