For Research Use Only. Not for use in diagnostic procedures.
仕様
アッセイRNA定量、高感度
励起/発光644/673
使用対象 (装置)蛍光光度計、マイクロプレートリーダー
反応数2000アッセイ
製品ラインQuant-iT
定量範囲5~100 ng
数量1 mL
出荷条件室温
検出法蛍光
Unit SizeEach
よくあるご質問(FAQ)
Why am I getting negative fluorescence values with my Qubit Assays?
Negative fluorescence is a physical impossibility. It is an artifact from software autocorrecting for background signal. This means your reader is picking up and subtracting out background light at the cost of your data. Make sure to do a buffer-only control and assess the type of signal. You may need to switch to a different plate.
引用および参考文献 (5)
引用および参考文献
Abstract
Icariin regulates the osteoblast differentiation and cell proliferation of MC3T3-E1 cells through microRNA-153 by targeting Runt-related transcription factor 2.
Authors:Huang Z, Cheng C, Wang J, Liu X, Wei H, Han Y, Yang S, Wang X
Journal:Exp Ther Med
PubMed ID:29904399
'Osteoporosis has become one of the most serious public health problems. Icariin, miR-153 and Runt-related transcription factor 2 (Runx2) have been demonstrated to regulate cell proliferation and differentiation in multiple cells. The aim of the present experiments was to investigate the potential mechanism underlying osteoblast differentiation and cell proliferation of ... More
Transcriptional Characterization Of The Tumor Immune Microenvironment And Its Prognostic Value For Locally Advanced Lung Adenocarcinoma In A Chinese Population.
Authors:Chen Y, Chen H, Mao B, Zhou Y, Shi X, Tang L, Jiang H, Wang G, Zhuang W
Journal:Cancer Manag Res
PubMed ID:31802941
'We investigated the relationship of the transcriptional tumor immune microenvironment with prognosis of patients with locally advanced lung adenocarcinoma (LUAD).'
Characterization of mast cell-derived rRNA-containing microvesicles and their inflammatory impact on endothelial cells.
Authors:Elsemüller AK, Tomalla V, Gärtner U, Troidl K, Jeratsch S, Graumann J, Baal N, Hackstein H, Lasch M, Deindl E, Preissner KT, Fischer S
Journal:FASEB J
PubMed ID:30702929
Tissue-resident mast cells (MCs) are well known for their role in inflammatory responses and allergic and anaphylactic reactions, but they also contribute to processes of arterial remodeling. Although ribosomes and cytosolic RNAs are located around secretory granules in mature MCs, their functional role in MC responses remains unexplored. Previous studies ... More
A novel long noncoding RNA FAF inhibits apoptosis via upregulating FGF9 through PI3K/AKT signaling pathway in ischemia-hypoxia cardiomyocytes.
Authors:Shi HJ, Wang MW, Sun JT, Wang H, Li YF, Chen BR, Fan Y, Wang SB, Wang ZM, Wang QM, Wang LS
Journal:J Cell Physiol
PubMed ID:31093967
Long noncoding RNAs (lncRNAs) have been increasingly considered to play an important role in the pathological process of various cardiovascular diseases, which often bind to the proximal promoters of the protein-coding gene to regulate the protein expression. However, the functions and mechanisms of lncRNAs in cardiomyocytes have not been fully ... More
Circulating RNA Transcriptome of Pregnant Women with TSH Just Above the Trimester-Specific Reference and its Correlation with the Hypertensive Phenotype.
Authors:Hirata AHL, Rocha LAJ, da Silva VA, de Almeida RJ, Bacigalupo LDS, Varela P, Martins L, Pesquero JB, Dellê H, Camacho CP
Journal:Sci Rep
PubMed ID:32296081
During gestation, a woman's body undergoes physiological changes that alter thyroid function. Pregnant women with hypothyroidism may exhibit gestational complications, including hypertension and preeclampsia. We investigated differentially expressed genes (DEGs) in circulating RNAs from pregnant women with TSH levels just above the normal range to determine the impact of a ... More