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Invitrogen™
Quant-iT™ dsDNA Assay Kits, high sensitivity (HS) and broad range (BR)
RNAを介した二本鎖DNAの高選択的定量を目的として、高感度かつ幅広い定量用のQuant-iT dsDNAアッセイキットは、それぞれ0.2∼100 ngおよび2∼1,000 ngのDNA範囲で直線的な蛍光シグナルを生成します。
製品番号（カタログ番号） Q33130
価格（JPY）お問い合わせください ›
139,100
Each
アッセイ:
dsDNA定量、広範囲
定量範囲:
4∼1,000 ng
Quant-iT dsDNAアッセイキットを使用すると、dsDNA定量を簡単かつ迅速に実行できます。Quant-iT High-Sensitivity dsDNA Assay KitおよびQuant-iT Broad-Range dsDNA Assay Kitには、濃縮アッセイ試薬、希釈バッファー、および希釈済みDNA標準液が含まれます。これらのDNAアッセイキットは、RNAを介したニ本鎖DNAに対して高い選択性を有し、0.2∼100 ng DNA（HS dsDNA Assay Kit用）および2∼1,000 ng DNA（BR dsDNA Assay Kit用）の範囲において蛍光シグナルは線形です。
Quant-iT dsDNA High-Sensitivity Assay KitおよびQuant-iT dsDNA Broad Range Assay Kitを使用すると、DNA定量を簡単かつ正確に行うことができます。キットには、濃縮アッセイ試薬、希釈バッファー、および希釈済みDNA標準液が含まれています。試薬を1：200で希釈し、200 μLをマイクロプレートのウェルに重点します。1～20 μLのサンプルを加えて混合し、蛍光を読み取ります。
このアッセイは、RNAよりも二本鎖DNAに対して高い選択性を有し、HSアッセイでは0.2～100 ng、BRアッセイでは2～1,000 ngの範囲において蛍光シグナルはDNAで線形となります。サンプル調製から測定まで室温で行うことができ、シグナルは3時間安定しています。これらのアッセイでは、塩、溶媒、界面活性剤、タンパク質などの一般的な汚染物質は十分に耐性があります。
このアッセイは、RNAよりも二本鎖DNAに対して高い選択性を有し、HSアッセイでは0.2～100 ng、BRアッセイでは2～1,000 ngの範囲において蛍光シグナルはDNAで線形となります。サンプル調製から測定まで室温で行うことができ、シグナルは3時間安定しています。これらのアッセイでは、塩、溶媒、界面活性剤、タンパク質などの一般的な汚染物質は十分に耐性があります。
For Research Use Only. Not for use in diagnostic procedures.
仕様
アッセイdsDNA定量、広範囲
使用対象 (装置)マイクロプレートリーダー
反応数1,000(200 μLアッセイ容量)
製品ラインQuant-iT
定量範囲4∼1,000 ng
数量1 kit
出荷条件室温
検出法蛍光
Unit SizeEach
よくあるご質問（FAQ）
Why am I getting negative fluorescence values with my Qubit Assays?
I have a Quant-iT DNA Kit and want to use it for the Qubit Fluorometer. Can I?
What is the useful pH range for Quant-iT DNA kits?
I'm trying to quantify some DNA labeled with a fluorophore. Will this work?
Does DNA length have an effect on the dsDNA assays?
引用および参考文献 (16)
引用および参考文献
Abstract
3D chromatin conformation correlates with replication timing and is conserved in resting cells.
Journal:Nucleic Acids Res
PubMed ID:22879376
'Although chromatin folding is known to be of functional importance to control the gene expression program, less is known regarding its interplay with DNA replication. Here, using Circular Chromatin Conformation Capture combined with high-throughput sequencing, we identified megabase-sized self-interacting domains in the nucleus of a human lymphoblastoid cell line, as
A significant effect of the TSPY1 copy number on spermatogenesis efficiency and the phenotypic expression of the gr/gr deletion.
Journal:Hum Mol Genet
PubMed ID:23307928
'AZFc deletions cause a significant phenotypic heterogeneity with respect to spermatogenesis; however, the reason for this is poorly understood. Recently, testis-specific protein Y-encoded 1 (TSPY1) copy number variation (CNV) was determined to be a potential genetic modifier of spermatogenesis. We performed a large-scale cohort study to investigate the effect of
Gene expression profiling in insulinomas of Men1 beta-cell mutant mice reveals early genetic and epigenetic events involved in pancreatic beta-cell tumorigenesis.
Journal:Endocr Relat Cancer
PubMed ID:17158767
'Mutations of the MEN1 gene lead to the occurrence of multiple endocrine neoplasia type 1 (MEN1). To gain insights into the mechanisms of the tumorigenesis related to MEN1 inactivation, we have used mice in which the Men1 gene was specifically disrupted in pancreatic beta-cells. In these mice, we observed full
Effects of essential oils on methane production and fermentation by, and abundance and diversity of, rumen microbial populations.
Journal:Appl Environ Microbiol
PubMed ID:22492451
'Five essential oils (EOs), namely, clove oil (CLO), eucalyptus oil (EUO), garlic oil (GAO), origanum oil (ORO), and peppermint oil (PEO), were tested in vitro at 3 different doses (0.25, 0.50, and 1.0 g/liter) for their effect on methane production, fermentation, and select groups of ruminal microbes, including total bacteria,
Normal variants of Microcephalin and ASPM do not account for brain size variability.
Journal:Hum Mol Genet
PubMed ID:16687438
'Normal human brain volume is heritable. The genes responsible for variation in brain volume are not known. Microcephalin (MCPH1) and ASPM (abnormal spindle-like microcephaly associated) have been proposed as candidate genes as mutations in both genes are associated with microcephaly, and common variants of each gene are apparently under strong