Quant-iT™ Protein Assay Kit

Citations & References (6)

Invitrogen™

Quant-iT™ Protein Assay Kit

Quant-iTタンパク質アッセイキットを使用すると、タンパク質の定量を簡単かつ正確に行えます。キットには、濃縮アッセイ試薬、希釈バッファー、および希釈済みBSA標準液が含まれています。試薬を希釈し、マイクロプレートのウェルに充填して、1～20 µLのサンプルを加えて混合し詳細を見る

製品番号（カタログ番号）	数量
Q33210	1 Kit

製品番号（カタログ番号） Q33210

価格（JPY）

140,500

お問い合わせください ›

1 Kit

一括またはカスタム形式をリクエストする

Quant-iTタンパク質アッセイキットを使用すると、タンパク質の定量を簡単かつ正確に行えます。キットには、濃縮アッセイ試薬、希釈バッファー、および希釈済みBSA標準液が含まれています。試薬を希釈し、マイクロプレートのウェルに充填して、1～20 µLのサンプルを加えて混合し、蛍光を測定するだけです。アッセイはタンパク質に対して高い選択性を示し、タンパク質間の変動はほとんどありません。アッセイは室温で行うことができ、シグナルは3時間安定しています。アッセイでは、塩類、溶媒、またはDNA（ただし界面活性剤は含まれない）などの一般的な汚染物質に対する耐性は十分に確保されます。Quant-iT DNAアッセイキット（Q33120、Q33130）およびQuant-iT RNAアッセイキット（Q33140）も使用できます。

研究用にのみ使用できます。診断用には使用いただけません。

アッセイタンパク質定量アッセイ

使用対象 (装置)マイクロプレートリーダー

遺伝子ID（Entrez）3919448

キット内容濃縮アッセイ試薬、希釈バッファー、および希釈済みBSA標準液

製品ラインQuant-iT

製品タイプProtein Quantitation Assay

数量1 Kit

サンプル量1～20 uL

出荷条件室温

保存要件冷蔵庫（2～8℃）で保管遮光。

検出法蛍光

Unit SizeEach

組成および保存条件

冷蔵庫（2～8℃）に保存し、遮光。

よくあるご質問（FAQ）

Why am I getting negative fluorescence values with my Qubit Assays?

Negative fluorescence is a physical impossibility. It is an artifact from software autocorrecting for background signal. This means your reader is picking up and subtracting out background light at the cost of your data. Make sure to do a buffer-only control and assess the type of signal. You may need to switch to a different plate.

I have a Quant-iT DNA Kit and want to use it for the Qubit Fluorometer. Can I?

Yes, the manual has directions for this application. You will use the 0 ng/µL lambda dsDNA HS standard to generate Standard #1. You will prepare a dilution of the 10 ng/µL lambda dsDNA HS standard to generate Standard #2. You then prepare the samples and compare them to this 2-point standard curve. The Quant-iT dsDNA BR Kit can be used in a similar manner.

What is the useful pH range for Quant-iT DNA kits?

The buffer included in the kit should assure the proper pH range, even if your DNA is at a pH outside of this range, since at least a 10-fold excess of kit buffer over sample is used in the assay.

I'm trying to quantify some DNA labeled with a fluorophore. Will this work?

PicoGreen dye and other fluorescence-based quantification reagents are not recommended for quantifying dye-conjugated nucleic acids. The attached dye molecules can interfere with either binding and/or fluorescence output of the quantification reagents.

Does DNA length have an effect on the dsDNA assays?

Strands that are roughly in the 20-mer range or shorter show a lower level of signal. For dsDNA samples that are composed of mostly short strands, the reagent may still be used, but one should use a dsDNA standard that is of comparable length as the sample.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

View all Quant-iT™ Protein Assay Kit FAQs

引用および参考文献 (6)

引用および参考文献

Abstract

Acrolein inhibits cytokine gene expression by alkylating cysteine and arginine residues in the NF-kappaB1 DNA binding domain.

Authors:Lambert C, Li J, Jonscher K, Yang TC, Reigan P, Quintana M, Harvey J, Freed BM,

Journal:J Biol Chem

PubMed ID:17491020

Cigarette smoke is a potent inhibitor of pulmonary T cell responses, resulting in decreased immune surveillance and an increased incidence of respiratory tract infections. The alpha,beta-unsaturated aldehydes in cigarette smoke (acrolein and crotonaldehyde) inhibited production of interleukin-2 (IL-2), IL-10, granulocyte-macrophage colony-stimulating factor, interferon-gamma, and tumor necrosis factor-alpha by human T ... More

Functional selection of phagocytosis-promoting genes: cell sorting-based selection.

Authors:Jeon H, Go Y, Seo M, Lee WH, Suk K,

Journal:J Biomol Screen

PubMed ID:20660795

Phagocytosis is a critical host defense mechanism that clears invading pathogens, apoptotic cells, and cell debris; it is an essential process for normal development, tissue remodeling, immune response, and inflammation. Here, a functional selection strategy was used to isolate novel phagocytosis-promoting genes. After the retroviral transfer of mouse brain cDNA ... More

Quantitation of protein.

Authors:Noble JE, Bailey MJ,

Journal:Methods Enzymol

PubMed ID:19892168

The measurement of protein concentration in an aqueous sample is an important assay in biochemistry research and development labs for applications ranging from enzymatic studies to providing data for biopharmaceutical lot release. Spectrophotometric protein quantitation assays are methods that use UV and visible spectroscopy to rapidly determine the concentration of ... More

A comparison of protein quantitation assays for biopharmaceutical applications.

Authors:Noble JE, Knight AE, Reason AJ, Di Matola A, Bailey MJ,

Journal:Mol Biotechnol

PubMed ID:17914170

Dye-based protein determination assays are widely used to estimate protein concentration, however various reports suggest that the response is dependent on the composition and sequence of the protein, limiting confidence in the resulting concentration estimates. In this study a diverse set of model proteins representing various sizes of protein and ... More

Targeted quantitation of overexpressed and endogenous cystic fibrosis transmembrane conductance regulator using multiple reaction monitoring tandem mass spectrometry and oxygen stable isotope dilution.

Authors:Jiang H, Ramos AA, Yao X,

Journal:Anal Chem

PubMed ID:19947594

Cystic fibrosis transmembrane conductance regulator (CFTR) functions as an ion channel in the apical plasma membrane of epithelial cells. Mutations in the gene coding for CFTR cause cystic fibrosis (CF). A major cellular dysfunction is insufficient apical plasma membrane expression of the protein. Its correction is important for developing new ... More

View all Quant-iT™ Protein Assay Kit citations