Qubit™ Protein and Protein Broad Range (BR) Assay Kits

Citations & References (23)

Invitrogen™

Qubit™ Protein and Protein Broad Range (BR) Assay Kits

Name: Qubit™ Protein and Protein Broad Range (BR) Assay Kits
SKU: Q33211

蛍光ベースのタンパク質定量アッセイ用のQubitタンパク質およびタンパク質BRアッセイキットを使用すると、タンパク質の高感度検出のほか、タンパク質間の低変動を実現します。

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製品番号（カタログ番号）	アッセイ	定量範囲	対応可能対象
Q33211	タンパク質定量アッセイ	12.5 μg/mL～5 mg/mL	100 Reactions
Q33212	タンパク質定量アッセイ	12.5 μg/mL～5 mg/mL	500 Reactions
A50668	タンパク質定量、広範囲	0.1～20 mg/mL	100 Reactions
A50669	タンパク質定量アッセイ、広範囲	0.1～20 mg/mL	500 Reactions

4 オプション

製品番号（カタログ番号） Q33211

価格（JPY）

17,000

Each

お問い合わせください ›

アッセイ:

タンパク質定量アッセイ

定量範囲:

12.5 μg/mL～5 mg/mL

対応可能対象:

100 Reactions

当社独自の2種類のQubitタンパク質定量キット：Qubitタンパク質アッセイは12.5 μg/mL∼5 mg/mLのタンパク質を、Qubitタンパク質BRアッセイキットは0.1∼20 mg/mLのタンパク質をそれぞれ定量します。どちらのアッセイも、多くの一般的な汚染物質に対応しており、試薬、塩、核酸を低減します。タンパク質BRアッセイキットは、界面活性剤にも耐性があります。

Qubit Protein Assay Kit
Qubit Protein Assay Kitは、各種のQubit蛍光光度計で使用できるように専用設計されています。このアッセイでは、1～20 μL範囲にあるサンプルを使用する場合、タンパク質間のばらつきが少ない12.5 μg⁄mL～5 mg⁄mLのサンプルを定量できます。

Qubit Protein Assay Kitは、タンパク質に対して高い選択性を有し、還元試薬の存在下でも高い精度を示しますが、多量の界面活性剤の存在下での精度は高くありません。還元試薬（DTT、β-メルカプトエタノールなど）、塩、遊離ヌクレオチド、アミノ酸、溶媒、またはDNAなどの一般的な汚染物質は、界面活性剤ではなく、アッセイで十分に耐性があります。その他の汚染物質の場合には、若干のプロトコル変更が必要です。

このキットには、濃縮アッセイ試薬、希釈バッファー、希釈済みBSA標準液が付属します。希釈バッファーでアッセイ試薬を希釈し、サンプル（許容量範囲は1 μL～20 μL）を添加した後、濃度を読み取ります

蛍光タンパク質の定量にはどの製品を選べばよいでしょうか？
•1～20サンプル：このQubit Protein Assay KitとQubit Fluorometerを併用します
• 20～2,000サンプル：Quant-iT Protein Assay Kitとマイクロプレートリーダーを併用します

Qubit Protein BR Assay Kit
Qubit Protein BR Assay Kitは、Qubit 4 Fluorometerを用いて、精製タンパク質、ライセート、血清、血漿、複合タンパク質のタンパク質濃度を迅速かつ簡便に信頼性の高い方法で測定します。このタンパク質キットは、幅広い範囲（0.1～20 mg/mL）で正確なタンパク質定量を実現し、ほとんどのサンプルを効率的に（希釈なしで）使用できるため、従来のタンパク質定量法に伴う推測作業や希釈手順が不要となります。このアッセイは、界面活性剤、還元試薬（DTT、β-メルカプトエタノールなど）、塩、核酸など、多くの一般的な汚染物質に対応します。

Qubit Protein BR Assay Kitの主な特長：
•広いダイナミックレンジ—0.1～20 mg/mL、希釈なしでサンプルを測定
• 2点標準曲線—時間のかかる標準的な調製を排除
• 自動濃度測定—タンパク質濃度を瞬時に取得；オフラインでの計算は不要
• 適合性—還元試薬、界面活性剤、その他の一般的なバッファー成分の存在下でも高精度

Qubit Protein BR Assay Kitには、2種類のキャリブレーション標準液、Protein BR Assay BufferおよびProtein BR Assay Reagentが付属します。150～160 μLのProtein BR Assay Bufferをサンプルの10または20 μLのサンプルに加え、さらに30 μLのProtein BR Assay Reagentを添加するだけです。室温で10分間インキュベートします。次に、サンプルをQubit 4機器に挿入し、濃度を読み取ります。

注：
1.Qubitアッセイキットは、Qubit 1.0、Qubit 2.0、Qubit 3、Qubit 4、Qubit Flexの各光度計で使用できます。
2.Qubit Protein BR Assay Kitは、Qubit 4 Fluorometer専用の蛍光ベースアッセイです。
3.500 μL薄壁透明PCRチューブ（カタログ番号Q32856）が必要ですが、Protein AssayおよびBR Assay Kitには含まれていません。

For Research Use Only. Not for use in diagnostic procedures.

仕様

アッセイタンパク質定量アッセイ

使用対象 (装置)Qubit蛍光光度計、マイクロプレートリーダー

製品ラインQuant-iT、Qubit

製品タイプProtein Quantification Assay

定量範囲12.5 μg/mL～5 mg/mL

数量100反応

出荷条件室温

対応可能対象100 Reactions

検出法蛍光

Unit SizeEach

よくあるご質問（FAQ）

How does the accuracy and sensitivity of the Qubit quantitation assays using the Qubit fluorometer compare to a microplate reader?

The accuracy and sensitivity of the Qubit quantitation assays are the same as that of a microplate reader. This was a requirement during product development. The detection limits for each Qubit kit can be found on the corresponding product manual, which can be found by searching our website by keyword or catalog number.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

Can the Qubit kits give an indication of sample quality in nucleic acid samples?

No. The Qubit DNA and RNA kits only quantify the amount of either DNA or RNA in the sample. The Qubit fluorometer cannot take absorbance readings to provide a A260/A280 ratio or detect protein in nucleic acid samples. This can be done with the NanoDrop instrument. If your sample contains protein or other contaminants that can affect the assay, it should be further purified.

If your sample may contain both DNA and RNA, one may use either (or both) the DNA and RNA Qubit kits and compare with samples treated with either RNase or DNase to get an accurate determination of DNA or RNA, respectively.

Can I use the Quant-iT and Qubit Kits with other fluorometers?

All Quant-iT and Qubit kits are compatible with all fluorometers and microplate readers that have the appropriate light sources and filters. You won't have access to the algorithm in the Qubit fluorometer for generating the standard curve provided by the instrument, instead, you must make a few dilutions of the highest standard DNA or RNA (Standard #2) in the Qubit kits to generate a standard curve with multiple data points.

Can I use the original Quant-iT Kits with the Qubit Fluorometer?

No, we do not recommend this. Some of the dyes in the original Quant-iT kits (those NOT listed as “for use with the Qubit fluorometer”) are not compatible with the Qubit Fluorometer. In addition, the new Quant-iT kits (labeled as “for use with the Qubit Fluorometer”) have standards formulated to be compatible with the Qubit fluorometer internal algorithms for the respective assays. The Qubit Fluorometer-compatible kits are also less expensive per assay if you are processing fewer than 20 samples at a time.

Is 1X RIPA buffer (50 mM Tris-HCl, 150 mM NaCl, 0.25% deoxycholic acid, 1% NP-40, 1mM EDTA, pH 7.4) compatible with the Qubit Protein Assay (Cat. No. Q33211/Q33212)?

The concentration of detergents in 1x RIPA buffer is quite high, therefore, we do not recommend that you use this kit with samples in RIPA. The Qubit Protein Assay has a tolerance for detergents present only in very low amounts. Please see the user guide for the tolerance of this assay for detergents here. The Qubit Protein Broad Range (BR) Assay Kit (A50668) has a much higher tolerance for detergents, e.g. RIPA buffer can be used undiluted. Please see this link.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

View all Qubit™ Protein and Protein Broad Range (BR) Assay Kits FAQs

引用および参考文献 (23)

引用および参考文献

Abstract

Post-training disruption of Arc protein expression in the anterior cingulate cortex impairs long-term memory for inhibitory avoidance training.

Authors:Holloway CM, McIntyre CK,

Journal:Neurobiol Learn Mem

PubMed ID:21315825

'The activity-regulated-cytoskeletal-associated protein (Arc) has a well established role in memory consolidation and synaptic plasticity in the hippocampus and amygdala. However the role of Arc within the anterior cingulate cortex (ACC), an area of the brain involved in processing memory for pain, has yet to be examined. Here we sought ... More

Investigation of the involvement of P-glycoprotein and multidrug resistance-associated protein 2 in the efflux of ximelagatran and its metabolites by using short hairpin RNA knockdown in Caco-2 cells.

Authors:Darnell M, Karlsson JE, Owen A, Hidalgo IJ, Li J, Zhang W, Andersson TB,

Journal:Drug Metab Dispos

PubMed ID:20023051

'Liver and bile secretion can be an important first-pass and clearance route for drug compounds and also the site of several drug-drug interactions. In the clinical program for ximelagatran development, an unexpected effect of erythromycin on the pharmacokinetics of the direct thrombin inhibitor ximelagatran and its metabolites was detected. This ... More

Synthesis and optimization of lectin functionalized nanoprobes for the selective recovery of glycoproteins from human body fluids.

Authors:Ferreira JA, Daniel-da-Silva AL, Alves RM, Duarte D, Vieira I, Santos LL, Vitorino R, Amado F,

Journal:Anal Chem

PubMed ID:21809823

'Biomedical sciences, and in particular biomarker research, demand efficient glycoprotein enrichment platforms. Herein magnetic nanoprobes (MNP), after being coated with three broad-spectrum lectins-concanavalin A (ConA), wheat germ agglutinin (WGA), and Maackia amurensis lectin (MA)-were utilized to selectively capture glycoproteins from human body fluids. Additionally, a new methodology, based on protection ... More

Active and total transforming growth factor-ß1 are differentially regulated by dopamine and estradiol in the pituitary.

Authors:Recouvreux MV, Guida MC, Rifkin DB, Becu-Villalobos D, Díaz-Torga G,

Journal:Endocrinology

PubMed ID:21521749

'Dopamine, acting through the dopamine type 2 receptor (Drd2), is the main inhibitor of pituitary prolactin (PRL) secretion and lactotroph proliferation. TGF-ß1 is involved, at least in part, in mediating these actions. It was described that TGF-ß1 synthesis in rat pituitary lactotrophs is up-regulated by dopamine and down-regulated by estradiol. ... More

Silencing of NHE-1 blunts the slow force response to myocardial stretch.

Authors:Pérez NG, Nolly MB, Roldan MC, Villa-Abrille MC, Cingolani E, Portiansky EL, Alvarez BV, Ennis IL, Cingolani HE,

Journal:J Appl Physiol (1985)

PubMed ID:21659487

'Myocardial stretch induces a biphasic force response: a first abrupt increase followed by a slow force response (SFR), believed to be the in vitro manifestation of the Anrep effect. The SFR is due to an increase in Ca²? transient of unclear mechanism. We proposed that Na?/H? exchanger (NHE-1) activation is ... More

View all Qubit™ Protein and Protein Broad Range (BR) Assay Kits citations