Qubit™ 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits

Citations & References (5)

Invitrogen™

Qubit™ 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits

Name: Qubit™ 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits
SKU: Q33230

Qubit 1X dsDNA HSおよびBRアッセイキットは、RNAを介したdsDNAに対して高い選択性を示し、100および500のアッセイフォーマットでdsDNAを正確に定量するうえで使いやすい組成内容となります。ラムダ標準液は、最大500サンプルに十分な量にある材料となります。

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製品番号（カタログ番号）	数量	アッセイ	定量範囲
Q33230	100 assays	1X dsDNA, high sensitivity	0.1～120ng
Q33231	500 assays	1X dsDNA, high sensitivity	0.1～120ng
Q33266	500 assays	1X dsDNA, broad range	4∼4000 ng
Q33265	100 assays	1X dsDNA, broad range	4∼4000 ng

4 オプション

製品番号（カタログ番号） Q33230

価格（JPY）

18,200

Each

お問い合わせください ›

数量:

100 assays

アッセイ:

1X dsDNA, high sensitivity

定量範囲:

0.1～120ng

Qubit 1X dsDNA HS（高感度）およびQubit 1X dsDNA BR（広範囲）Assay Kitを使用して、dsDNAを簡単、正確、精密に定量できます。等倍組成は、Qubit FluorometerでdsDNAサンプルを簡単に定量できる、すぐに使用可能な標準溶液です。これらのアッセイは、ssDNA、RNA、タンパク質、遊離ヌクレオチドよりもdsDNAを高い選択性で検出します。塩、溶媒、界面活性剤などの汚染物質に対し、十分に耐性があります。

Qubit 1X dsDNA HSおよびBR Assay Kitは、Qubit Fluorometer用に設計されており、一本鎖DNA（ssDNA）、RNA、タンパク質および遊離ヌクレオチドよりも二本鎖DNA（dsDNA）に高い選択性を有しています。すべてのキットには、すぐに使用可能な標準溶液とDNA標準が含まれています。アッセイを実施するには、サンプル（1～20 μLの任意の容量が許容されます）を等倍の標準溶液に希釈し、Qubit Fluorometerを使用して濃度を読み取るだけです。

Qubit 1X dsDNA HS Assay Kit
Qubit dsDNA HS（高感度）Assay KitをQubit Fluorometerと併用することで、高感度DNAサンプルの定量を正確かつ選択的に行うことができます。サンプル量に応じて、このアッセイキットはDNAサンプルの初期濃度が5 pg/μL～120 ng/μLで、0.1～120 ngの検出範囲を提供するように正確に設計されています。

Qubit 1X dsDNA BR Assay Kit
Qubit dsDNA BR（広範囲）Assay Kitは、Qubit 4またはQubit Flex Fluorometerと併用するよう設計されており、DNAサンプルの正確かつ選択的な定量が可能となります。サンプル量に応じて、このアッセイキットはDNAサンプルの初期濃度が0.2～4,000 ng/μLで正確となるように設計されており、4～4,000 ngの検出範囲を実現します。

注：
•    Qubit 1X dsDNA HSは、Qubit 2、Qubit 3、Qubit 4、およびQubit Flex Fluorometersで使用できます。
•   Qubit 1X dsDNA BRは、Qubit 4およびQubit Flex Fluorometersで使用するよう設計されています。
•    薄壁、透明、0.5 mL PCRチューブ（カタログ番号Q32856）をQubit 4 Fluorometerに使用、8 × 200 μLのチューブストリップ（カタログ番号Q33252）を使用してください。

For Research Use Only. Not for use in diagnostic procedures.

仕様

アッセイ1X dsDNA, high sensitivity

使用対象 (装置)Qubit 2.0、3、4、およびFlex蛍光光度計

グリーン機能使用するリソースが少なく、廃棄物も少なくて済みます

反応数100反応

製品ラインQubit

定量範囲0.1～120ng

数量100 assays

出荷条件湿氷

Unit SizeEach

よくあるご質問（FAQ）

What is the expiration date for Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits (Cat. Nos. Cat. Q33230, Q33231, Q33265, Q33266)?

The Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits (Cat. Nos. Cat. Q33230, Q33231, Q33265, Q33266) are stable for 6 months from the date of receipt when stored as directed.

For more information on product storage, please refer to the user guides:

Qubit 1X dsDNA HS Assay Kit and Qubit 1X dsDNA BR Assay Kit

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

What is the difference between The Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits (Cat. Nos. Q33230, Q33231, Q33265, Q33366) and Qubit dsDNA Quantification Assay Kits (Cat. Nos. Q32850, Q32851, Q32853, Q32854)?

The Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits (Cat. Nos. Q33230, Q33231, Q33265, Q33366) are newer and have a simplified workflow compared to the original Qubit dsDNA Quantification Assay Kits (Cat. Nos. Q32850, Q32851, Q32853, Q32854). The original Qubit kits contain separate dyes and buffer components that must be mixed together to make a working solution, in which the dye degrades after a few hours. With the newer Qubit 1X dsDNA Assay kits, the dye is premixed with a buffer that keeps the dye stable long-term and can be added directly to DNA samples.

You can find more information about the Qubit 1X dsDNA assay, by clicking on the Technical Note provided below:

Qubit 1X dsDNA assays: simplified workflow and improved performance

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

How should I store my Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits (Cat. No. Q33230, Q33231, Q33265, Q33266)?

The Qubit 1X dsDNA High Sensitivity (HS) (Cat. Nos. Q33230, Q33231) and Broad Range (BR) Assay Kits (Cat. Nos. Q33265, Q33266) have different storage conditions.

For Qubit 1X dsDNA HS Assay Kits (Cat. Nos. Q33230, Q33231), store all components at 2 - 8 degrees C.

For Qubit 1X dsDNA BR Assay Kits (Cat. Nos. Q33265, Q33266), store the working solution at 18 - 28 degrees C. Store the standards at 2 - 8 degrees C.

The assay kits are stable for at least 6 months from the date of receipt.

For more information, please refer to these user guides: Qubit 1X dsDNA HS Assay Kit and Qubit 1X dsDNA BR Assay Kit

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

引用および参考文献 (5)

引用および参考文献

Abstract

Prevalence of Germline Mutations Associated With Cancer Risk in Patients With Intraductal Papillary Mucinous Neoplasms.

Authors:Skaro M, Nanda N, Gauthier C, Felsenstein M, Jiang Z, Qiu M, Shindo K, Yu J, Hutchings D, Javed AA, Beckman R, He J, Wolfgang CL, Thompson E, Hruban RH, Klein AP, Goggins M, Wood LD, Roberts NJ

Journal:Gastroenterology

PubMed ID:30716324

'Many patients with pancreatic adenocarcinoma carry germline mutations associated with increased risk of cancer. It is not clear whether patients with intraductal papillary mucinous neoplasms (IPMNs), which are precursors to some pancreatic cancers, also carry these mutations. We assessed the prevalence of germline mutations associated with cancer risk in patients ... More

Unravelling the Role of Trophoblastic-Derived Extracellular Vesicles in Regulatory T Cell Differentiation.

Authors:Kovács ÁF, Fekete N, Turiák L, Ács A, Kohidai L, Buzás EI, Pállinger É

Journal:Int J Mol Sci

PubMed ID:31337116

'Regulatory T cells (T'

Genomic Epidemiology of SARS-CoV-2 in Guangdong Province, China.

Authors:Lu J, du Plessis L, Liu Z, Hill V, Kang M, Lin H, Sun J, François S, Kraemer MUG, Faria NR, McCrone JT, Peng J, Xiong Q, Yuan R, Zeng L, Zhou P, Liang C, Yi L, Liu J, Xiao J, Hu J, Liu T, Ma W, Li W, Su J, Zheng H, Peng B, Fang S, Su W, Li K, Sun R, Bai R, Tang X, Liang M, Quick J, Song T, Rambaut A, Loman N, Raghwani J, Pybus OG, Ke C

Journal:Cell

PubMed ID:32359424

'Coronavirus disease 2019 (COVID-19) is caused by SARS-CoV-2 infection and was first reported in central China in December 2019. Extensive molecular surveillance in Guangdong, China''s most populous province, during early 2020 resulted in 1,388 reported RNA-positive cases from 1.6 million tests. In order to understand the molecular epidemiology and genetic ... More

The Application of Nanopore Sequencing Technology to the Study of Dinoflagellates: A Proof of Concept Study for Rapid Sequence-Based Discrimination of Potentially Harmful Algae.

Authors:Hatfield RG, Batista FM, Bean TP, Fonseca VG, Santos A, Turner AD, Lewis A, Dean KJ, Martinez-Urtaza J

Journal:Front Microbiol

PubMed ID:32457722

'Harmful algal blooms (HABs) are a naturally occurring global phenomena that have the potential to impact fisheries, leisure and ecosystems, as well as posing a significant hazard to animal and human health. There is significant interest in the development and application of methodologies to study all aspects of the causative ... More

Integration of Genomic and Transcriptional Features in Pancreatic Cancer Reveals Increased Cell Cycle Progression in Metastases.

Authors:Connor AA, Denroche RE, Jang GH, Lemire M, Zhang A, Chan-Seng-Yue M, Wilson G, Grant RC, Merico D, Lungu I, Bartlett JMS, Chadwick D, Liang SB, Eagles J, Mbabaali F, Miller JK, Krzyzanowski P, Armstrong H, Luo X, Jorgensen LGT, Romero JM, Bavi P, Fischer SE, Serra S, Hafezi-Bakhtiari S, Caglar D, Roehrl MHA, Cleary S, Hollingsworth MA, Petersen GM, Thayer S, Law CHL, Nanji S, Golan T, Smith AL, Borgida A, Dodd A, Hedley D, Wouters BG, O'Kane GM, Wilson JM, Zogopoulos G, Notta F, Knox JJ, Galling

Journal:Cancer Cell

PubMed ID:30686769

We integrated clinical, genomic, and transcriptomic data from 224 primaries and 95 metastases from 289 patients to characterize progression of pancreatic ductal adenocarcinoma (PDAC). Driver gene alterations and mutational and expression-based signatures were preserved, with truncations, inversions, and translocations most conserved. Cell cycle progression (CCP) increased with sequential inactivation of ... More