Qubit™ 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits

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Invitrogen™

Qubit™ 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits

Name: Qubit™ 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits
SKU: Q33231

Qubit 1X dsDNA HSおよびBRアッセイキットは、RNAを介したdsDNAに対して高い選択性を示し、100および500のアッセイフォーマットでdsDNAを正確に定量するうえで使いやすい組成内容となります。ラムダ標準液は、最大500サンプルに十分な量にある材料となります。

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製品番号（カタログ番号）	数量	アッセイ	定量範囲
Q33231	500 assays	1X dsDNA, high sensitivity	0.1～120ng
Q33230	100 assays	1X dsDNA, high sensitivity	0.1～120ng
Q33266	500 assays	1X dsDNA, broad range	4∼4000 ng
Q33265	100 assays	1X dsDNA, broad range	4∼4000 ng

4 オプション

製品番号（カタログ番号） Q33231

価格（JPY）

54,200

Each

お問い合わせください ›

数量:

500 assays

アッセイ:

1X dsDNA, high sensitivity

定量範囲:

0.1～120ng

Qubit 1X dsDNA HS（高感度）およびQubit 1X dsDNA BR（広範囲）Assay Kitを使用して、dsDNAを簡単、正確、精密に定量できます。等倍組成は、Qubit FluorometerでdsDNAサンプルを簡単に定量できる、すぐに使用可能な標準溶液です。これらのアッセイは、ssDNA、RNA、タンパク質、遊離ヌクレオチドよりもdsDNAを高い選択性で検出します。塩、溶媒、界面活性剤などの汚染物質に対し、十分に耐性があります。

Qubit 1X dsDNA HSおよびBR Assay Kitは、Qubit Fluorometer用に設計されており、一本鎖DNA（ssDNA）、RNA、タンパク質および遊離ヌクレオチドよりも二本鎖DNA（dsDNA）に高い選択性を有しています。すべてのキットには、すぐに使用可能な標準溶液とDNA標準が含まれています。アッセイを実施するには、サンプル（1～20 μLの任意の容量が許容されます）を等倍の標準溶液に希釈し、Qubit Fluorometerを使用して濃度を読み取るだけです。

Qubit 1X dsDNA HS Assay Kit
Qubit dsDNA HS（高感度）Assay KitをQubit Fluorometerと併用することで、高感度DNAサンプルの定量を正確かつ選択的に行うことができます。サンプル量に応じて、このアッセイキットはDNAサンプルの初期濃度が5 pg/μL～120 ng/μLで、0.1～120 ngの検出範囲を提供するように正確に設計されています。

Qubit 1X dsDNA BR Assay Kit
Qubit dsDNA BR（広範囲）Assay Kitは、Qubit 4またはQubit Flex Fluorometerと併用するよう設計されており、DNAサンプルの正確かつ選択的な定量が可能となります。サンプル量に応じて、このアッセイキットはDNAサンプルの初期濃度が0.2～4,000 ng/μLで正確となるように設計されており、4～4,000 ngの検出範囲を実現します。

注：
•    Qubit 1X dsDNA HSは、Qubit 2、Qubit 3、Qubit 4、およびQubit Flex Fluorometersで使用できます。
•   Qubit 1X dsDNA BRは、Qubit 4およびQubit Flex Fluorometersで使用するよう設計されています。
•    薄壁、透明、0.5 mL PCRチューブ（カタログ番号Q32856）をQubit 4 Fluorometerに使用、8 × 200 μLのチューブストリップ（カタログ番号Q33252）を使用してください。

For Research Use Only. Not for use in diagnostic procedures.

仕様

アッセイ1X dsDNA, high sensitivity

使用対象 (装置)Qubit 2.0、3、4、およびFlex蛍光光度計

グリーン機能使用するリソースが少なく、廃棄物も少なくて済みます

反応数500反応

製品ラインQubit

定量範囲0.1～120ng

数量500 assays

出荷条件湿氷

Unit SizeEach

よくあるご質問（FAQ）

What is the expiration date for Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits (Cat. Nos. Cat. Q33230, Q33231, Q33265, Q33266)?

The Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits (Cat. Nos. Cat. Q33230, Q33231, Q33265, Q33266) are stable for 6 months from the date of receipt when stored as directed.

For more information on product storage, please refer to the user guides:

Qubit 1X dsDNA HS Assay Kit and Qubit 1X dsDNA BR Assay Kit

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

What is the difference between The Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits (Cat. Nos. Q33230, Q33231, Q33265, Q33366) and Qubit dsDNA Quantification Assay Kits (Cat. Nos. Q32850, Q32851, Q32853, Q32854)?

The Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits (Cat. Nos. Q33230, Q33231, Q33265, Q33366) are newer and have a simplified workflow compared to the original Qubit dsDNA Quantification Assay Kits (Cat. Nos. Q32850, Q32851, Q32853, Q32854). The original Qubit kits contain separate dyes and buffer components that must be mixed together to make a working solution, in which the dye degrades after a few hours. With the newer Qubit 1X dsDNA Assay kits, the dye is premixed with a buffer that keeps the dye stable long-term and can be added directly to DNA samples.

You can find more information about the Qubit 1X dsDNA assay, by clicking on the Technical Note provided below:

Qubit 1X dsDNA assays: simplified workflow and improved performance

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

How should I store my Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits (Cat. No. Q33230, Q33231, Q33265, Q33266)?

The Qubit 1X dsDNA High Sensitivity (HS) (Cat. Nos. Q33230, Q33231) and Broad Range (BR) Assay Kits (Cat. Nos. Q33265, Q33266) have different storage conditions.

For Qubit 1X dsDNA HS Assay Kits (Cat. Nos. Q33230, Q33231), store all components at 2 - 8 degrees C.

For Qubit 1X dsDNA BR Assay Kits (Cat. Nos. Q33265, Q33266), store the working solution at 18 - 28 degrees C. Store the standards at 2 - 8 degrees C.

The assay kits are stable for at least 6 months from the date of receipt.

For more information, please refer to these user guides: Qubit 1X dsDNA HS Assay Kit and Qubit 1X dsDNA BR Assay Kit

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

引用および参考文献 (7)

引用および参考文献

Abstract

Plasmodium-specific atypical memory B cells are short-lived activated B cells.

Authors:Pérez-Mazliah D, Gardner PJ, Schweighoffer E, McLaughlin S, Hosking C, Tumwine I, Davis RS, Potocnik AJ, Tybulewicz VL, Langhorne J,

Journal:Elife

PubMed ID:30387712

'A subset of atypical memory B cells accumulates in malaria and several infections, autoimmune disorders and aging in both humans and mice. It has been suggested these cells are exhausted long-lived memory B cells, and their accumulation may contribute to poor acquisition of long-lasting immunity to certain chronic infections, such ... More

Co-digestion of organic and mineral wastes for enhanced biogas production: Reactor performance and evolution of microbial community and function.

Authors:Shamurad B, Gray N, Petropoulos E, Tabraiz S, Acharya K, Quintela-Baluja M, Sallis P

Journal:Waste Manag

PubMed ID:31109531

'Mineral wastes (MWs) from municipal solid waste incineration plants and construction demolition sites are rich in minerals, heavy metals and have acid neutralising capacity. This renders such MWs a promising source of bulk and trace elements to enhance and stabilize biogas production in anaerobic processes. However, finding a MW with ... More

Unravelling the Role of Trophoblastic-Derived Extracellular Vesicles in Regulatory T Cell Differentiation.

Authors:Kovács ÁF, Fekete N, Turiák L, Ács A, Kohidai L, Buzás EI, Pállinger É

Journal:Int J Mol Sci

PubMed ID:31337116

'Regulatory T cells (T'

Genomic Epidemiology of SARS-CoV-2 in Guangdong Province, China.

Authors:Lu J, du Plessis L, Liu Z, Hill V, Kang M, Lin H, Sun J, François S, Kraemer MUG, Faria NR, McCrone JT, Peng J, Xiong Q, Yuan R, Zeng L, Zhou P, Liang C, Yi L, Liu J, Xiao J, Hu J, Liu T, Ma W, Li W, Su J, Zheng H, Peng B, Fang S, Su W, Li K, Sun R, Bai R, Tang X, Liang M, Quick J, Song T, Rambaut A, Loman N, Raghwani J, Pybus OG, Ke C

Journal:Cell

PubMed ID:32359424

'Coronavirus disease 2019 (COVID-19) is caused by SARS-CoV-2 infection and was first reported in central China in December 2019. Extensive molecular surveillance in Guangdong, China''s most populous province, during early 2020 resulted in 1,388 reported RNA-positive cases from 1.6 million tests. In order to understand the molecular epidemiology and genetic ... More

High-throughput isolation of giant viruses using high-content screening.

Authors:Francis R, Ominami Y, Bou Khalil JY, La Scola B,

Journal:Commun Biol

PubMed ID:31240254

The race to discover and isolate giant viruses began 15 years ago. Metagenomics is counterbalancing coculture, with the detection of giant virus genomes becoming faster as sequencing technologies develop. Since the discovery of giant viruses, many efforts have been made to improve methods for coculturing amebas and giant viruses, which ... More

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