ViewRNA™ ISH Cell Assay Kit

Citations & References (27)

Invitrogen™

ViewRNA™ ISH Cell Assay Kit

ViewRNA™ ISH Cell Assayは直接蛍光RNA in situハイブリダイゼーション法で蛍光顕微鏡またはハイコンテントイメージャーを使用して、単一コピー感度および単一細胞分解能で1 ∼ 4つのRNAターゲット（mRNAまたはノンコーディングRNA詳細を見る

製品番号（カタログ番号）	数量
QVC0001	1 kit

製品番号（カタログ番号） QVC0001

価格（JPY）

347,000

お問い合わせください ›

1 kit

ViewRNA™ ISH Cell Assayは直接蛍光RNA in situハイブリダイゼーション法で蛍光顕微鏡またはハイコンテントイメージャーを使用して、単一コピー感度および単一細胞分解能で1 ∼ 4つのRNAターゲット（mRNAまたはノンコーディングRNA）を同時に検出できます。一般的に高バックグラウンドと低感度によって制限される従来のFISH法とは異なり、このアッセイではターゲット特異的プローブセット（RNA FISHプローブ）および分岐DNAシグナル増幅（bDNA）に独自の化学を使用して特定のシグナルを検出します。蛍光RNA in situハイブリダイゼーションアッセイには主に4つのステップがあります：サンプル調製、標的ハイブリダイゼーション、シグナル増幅、検出です。このViewRNA ISHアッセイキットは最大3つのRNAを検出できますが、ViewRNA™ 740モジュールと組み合わせると4番目のプローブを追加できます。

必要な追加コンポーネント：
10X PBS（カタログ番号QVC0508）
ViewRNA™-界面活性剤溶液QC（カタログ番号QVC0509）
ViewRNA™-洗浄バッファーセット（カタログ番号QG0507）
ViewRNA™ISHセルアクセサリキット（カタログ番号QVC0700）は、アッセイを実施するために試薬キットに含まれていない多くの必要なコンポーネントを提供することを目的としています。
ViewRNA™プローブセットはアッセイに含まれず、ViewRNA ISH細胞アッセイで使用するように設計されています。当社のウェブサイトにアクセスして、6,500種類を超える合成プローブセットの完全なリストをご覧ください。新しいプローブセットは、ご要望に応じて、追加コストなしで2週間以内に設計および合成できます。

4番目のRNAプローブを追加するには：
ViewRNA™ ISH Cell 740モジュール（カタログ番号QVC0200）はViewRNA™ ISH Cell Assayと組み合わせて使用するように設計されており、740チャンネル（AlexaFluor 750）で追加のRNAターゲットの解析が可能です。キットに含まれるすべての材料のリストについては、添付文書を参照してください。

報告済みアプリケーション
顕微鏡

研究用途にのみご使用ください。診断目的には使用できません。

数量1 kit

タイプISH Cell Assay Kit

Unit SizeEach

組成および保存条件

24ウェルプレートフォーマット（24サンプル）での1-plexから3-plex（AlexaFluor 488、546、647）アッセイに十分なコンポーネント、ガラススライドに取り付けられたカバースリップを使用する場合は96アッセイ

よくあるご質問（FAQ）

How do ViewRNA assays compare to RNAScope assays?

ViewRNA and RNAScope technologies rely on the same signal amplification strategy - branched DNA amplification. Historically, both ViewRNA and RNAScope technologies originated from the same company, Panomics. The assays are expected to yield similar sensitivity and resolution, however each technology relies on its own set of proprietary reagents and probe set designs. Hence, the assays are not considered interchangeable or compatible. ViewRNA probe sets are not tested for RNAScope assaya and vice versa.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is the signal amplified in ViewRNA assays?

The ViewRNA technology relies on branched DNA signal amplification strategy. Target probes complementary to the target transcript sequence are further hybridized with pre-amplifier, amplifier and label probes that consist of branched DNA, and form 'tree branches' that allow numerous label probes to attach. This approach allows higher signal amplification compared to traditional ISH techniques.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Where can I find general information about ViewRNA ISH Assays?

For general information about ViewRNA ISH Assays, please go to this page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/in-situ-hybridization-ish/rna-fish/viewrna-assays.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

For ViewRNA assays, can I use the same set of wash solutions for all samples, including the positive and negative controls?

We do not recommend doing this. The negative control should be processed and washed separately from the rest of the samples. This is because the negative control does not contain any target probe sets and only the amplification reagents are added to it. If experimental samples are washed in the same beaker of wash solutions as the negative control, any unbound target probes that wash away can carry over to the negative control sample and cause unexpected positive signal (that will also appear to be very specific).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The ViewRNA ISH Cell Assay Kit user manual lists an accessory kit, Cat. No. QVC0700 which has been discontinued. Do you offer or suggest alternatives for the items in the accessory kit that have also been discontinued?

Please contact our Technical Support team at cellanalysis.support@thermofisher.com for the list of suggested alternatives.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all ViewRNA™ ISH Cell Assay Kit FAQs

引用および参考文献 (27)

引用および参考文献

Abstract

Raphe serotonin neuron-specific oxytocin receptor knockout reduces aggression without affecting anxiety-like behavior in male mice only.

Authors:Pagani JH, Williams Avram SK, Cui Z, Song J, Mezey É, Senerth JM, Baumann MH, Young WS

Journal:

PubMed ID:25677455

'Serotonin and oxytocin influence aggressive and anxiety-like behaviors, though it is unclear how the two may interact. That the oxytocin receptor is expressed in the serotonergic raphe nuclei suggests a mechanism by which the two neurotransmitters may cooperatively influence behavior. We hypothesized that oxytocin acts on raphe neurons to influence ... More

Frequent proviral integration of the human betaretrovirus in biliary epithelium of patients with autoimmune and idiopathic liver disease.

Authors:Wang W, Indik S, Wasilenko ST, Faschinger A, Carpenter EJ, Tian Z, Zhang Y, Wong GK, Mason AL

Journal:

PubMed ID:25521721

'A human betaretrovirus (HBRV) has been linked with primary biliary cirrhosis (PBC) following the detection of viral particles in biliary epithelium by electron microscopy and cloning of the betaretrovirus genome from biliary epithelium and peri-hepatic lymph nodes. Evidence for viral infection was found in the majority of PBC patients'' peri-hepatic ... More

High-throughput detection of miRNAs and gene-specific mRNA at the single-cell level by flow cytometry.

Authors:Porichis F, Hart MG, Griesbeck M, Everett HL, Hassan M, Baxter AE, Lindqvist M, Miller SM, Soghoian DZ, Kavanagh DG, Reynolds S, Norris B, Mordecai SK, Nguyen Q, Lai C, Kaufmann DE

Journal:

PubMed ID:25472703

Fluorescent in situ hybridization (FISH) is a method that uses fluorescent probes to detect specific nucleic acid sequences at the single-cell level. Here we describe optimized protocols that exploit a highly sensitive FISH method based on branched DNA technology to detect mRNA and miRNA in human leukocytes. This technique can ... More

RNA sequencing of pancreatic circulating tumour cells implicates WNT signalling in metastasis.

Authors:Yu M, Ting DT, Stott SL, Wittner BS, Ozsolak F, Paul S, Ciciliano JC, Smas ME, Winokur D, Gilman AJ, Ulman MJ, Xega K, Contino G, Alagesan B, Brannigan BW, Milos PM, Ryan DP, Sequist LV, Bardeesy N, Ramaswamy S, Toner M, Maheswaran S, Haber DA

Journal:Nature

PubMed ID:22763454

Circulating tumour cells (CTCs) shed into blood from primary cancers include putative precursors that initiate distal metastases. Although these cells are extraordinarily rare, they may identify cellular pathways contributing to the blood-borne dissemination of cancer. Here, we adapted a microfluidic device for efficient capture of CTCs from an endogenous mouse ... More

Urinary Xist is a potential biomarker for membranous nephropathy.

Authors:Huang YS, Hsieh HY, Shih HM, Sytwu HK, Wu CC

Journal:

PubMed ID:25157805

Membranous nephropathy (MN), a type of glomerular nephritis, is the most common cause of nephrotic syndrome in human adults. Changes in gene expression as a result of epigenetic dysregulation through long noncoding RNAs (lncRNAs) are increasingly being recognized as important factors in disease. Using an experimental MN mouse model, we ... More

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