Does Diploid Growth Serum-Reduced Medium (SRM) contain L-glutamine?
The basal Diploid Growth Serum-Reduced Medium already contains 6 mM L-glutamine.
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Do I have to adjust my infection parameters when using Diploid Production Serum-Free Medium (SFM)?
We recommend evaluating performance with Diploid Production Serum-Free Medium using current conditions, however, multiplicity of infection, time of infection, and time of harvest may be different than with conventional media. Closely monitor cells for cytopathic effect and evaluate viral titers at multiple time points.
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Can Diploid Growth Serum-Reduced Medium (SRM) be supplemented with different types of serum?
Diploid Growth Serum-Reduced Medium can be supplemented with Fetal Bovine Serum, Newborn Calf Serum, Donor Bovine Serum with Iron, or Bovine Serum.
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Which cell lines can I culture in Diploid Growth Serum-Reduced Medium (SRM)?
Diploid Growth Serum-Reduced Medium has been evaluated with fibroblast cell lines, including MRC-5, WI-38, IMR-90, BS-2, and CEF cells.
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How long can I use Diploid Growth Serum-Reduced Medium (SRM) supplemented with Diploid Growth Supplement?
Once supplemented, the complete Diploid Growth Serum-Reduced Medium must be used within 4 weeks.
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