Quant-it™ RiboGreen Reagent and RNA Assay Kit
Use 96- and 384-well Microplates for Fluorescence-based Assays with Quant-iT assays for optimal results
For accurate quantification and a streamlined workflow try our new Quant-iT RiboGreen ReadyPlates!
Quant-it™ RiboGreen Reagent and RNA Assay Kit
Citations & References (54)
Invitrogen™

Quant-it™ RiboGreen Reagent and RNA Assay Kit

Quant-iT RiboGreen RNA Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. The Quant-iT RiboGreen RNA Assay Kit comes with reagent, dilution buffer, and rRNA standards to provide a linear detection range of 1–200 ng of RNA.
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製品番号（カタログ番号）数量製品タイプ定量範囲
R114901 kitQuant-iT RiboGreen RNA Kit1～200 ng
R114911 mLQuant-iT RiboGreen RNA試薬1～200 ng
R327001 kitRediPlate 96 RiboGreen RNA Kit3～200 ng
製品番号（カタログ番号） R11490
価格（JPY）
165,000
Each
お問い合わせください ›
数量:
1 kit
製品タイプ:
Quant-iT RiboGreen RNA Kit
定量範囲:
1～200 ng
Quant-iT RiboGreen RNA Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. The Quant-iT RiboGreen RNA Assay Kit comes with reagent, dilution buffer, and rRNA standards to provide a linear detection range of 1–200 ng of RNA.

Workflow 

This easy-to-use reagent offers two detection ranges: a high-assay range and a low-assay range for sensitive and broad range detection.  To use, simply prepare the working solution by diluting the provided dye with TE-Buffer, then aliquotting 100 µL of the working solution with standards and samples for a total reaction volume of 200 µL.  RNA is then quantitated using a fluorescence microplate reader (such as Varioskan ALF) at standard fluorescein wavelengths.

Applications

Detecting and quantitating small amounts of RNA is important in many applications including measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot analyses, S1 nuclease assays, RNase protection assays, cDNA library preparation, reverse transcription PCR, and differential display PCR.

For Research Use Only. Not for use in diagnostic procedures.
仕様
励起／発光500/525
使用対象 (装置)マイクロプレートリーダー
反応数2 mLを使用した200～2,000のキュベットアッセイ
製品ラインQuant-iT、RIBOGREEN
製品タイプQuant-iT RiboGreen RNA Kit
定量範囲1～200 ng
数量1 kit
出荷条件室温
検出法蛍光
Unit SizeEach

よくあるご質問（FAQ）

Why am I getting negative fluorescence values with my Qubit Assays?

Negative fluorescence is a physical impossibility. It is an artifact from software autocorrecting for background signal. This means your reader is picking up and subtracting out background light at the cost of your data. Make sure to do a buffer-only control and assess the type of signal. You may need to switch to a different plate.

What are the linear ranges of the Quant-iT RNA kits?

The linear ranges are:

- Quant-iT RiboGreen RNA Assay: 1 ng/mL to 1 µg/mL
- Quant-iT RNA BR Assay: 20-1,000 ng
- Quant-iT RNA HS Assay: 5-100 ng

What is the difference between the Quant-iT RNA assay and the Quant-iT RiboGreen RNA Assay?

There are several differences:

- The Quant-iT RNA Assay uses a far-red dye (excitation 644 nm/emission 673 nm); the Quant-iT RiboGreen RNA assay uses a green fluorescent dye (excitation 500 nm/emission 525 nm).
- The Quant-iT RNA assay is more specific for RNA.
- Quant-iT RNA Assays are less sensitive than the Quant-iT RiboGreen RNA Assay.

Can I make my own assay for the Qubit Fluorometer?

Yes, you can, for Qubit instruments developed after the original Qubit (1.0) Fluorometer. See MyQubit assay instructions here (http://www.thermofisher.com/us/en/home/life-science/laboratory-instruments/fluorometers/qubit/qubit-assays/myqubit.html.html).

I have a crude lysate. Will the Quant-iT and Qubit assays work?

Generally, the cleaner the sample the better. Some salts, proteins, and detergents are tolerated in the assays; see the specific assay protocol for which ones and at what concentrations.

View all Quant-it™ RiboGreen Reagent and RNA Assay Kit FAQs

引用および参考文献 (54)

引用および参考文献
Abstract
microRNAs exhibit high frequency genomic alterations in human cancer.
Authors:Zhang L,Huang J,Yang N,Greshock J,Megraw MS,Giannakakis A,Liang S,Naylor TL,Barchetti A,Ward MR,Yao G,Medina A,O'brien-Jenkins A,Katsaros D,Hatzigeorgiou A,Gimotty PA,Weber BL,Coukos G
Journal:Proceedings of the National Academy of Sciences of the United States of America
PubMed ID:16754881
MicroRNAs (miRNAs) are endogenous noncoding RNAs, which negatively regulate gene expression. To determine genomewide miRNA DNA copy number abnormalities in cancer, 283 known human miRNA genes were analyzed by high-resolution array-based comparative genomic hybridization in 227 human ovarian cancer, breast cancer, and melanoma specimens. A high proportion of genomic loci ... More
A fluorescence-based assay for multisubunit DNA-dependent RNA polymerases.
Authors:Kuhlman P, Duff HL, Galant A
Journal:Anal Biochem
PubMed ID:14690681
The properties of DNA-dependent RNA polymerases have been studied since the 1960s, but considerable interest in probing RNA polymerase structure/function relationships, the roles of different classes of RNA polymerases in cellular processes, and the feasibility of using RNA polymerases as drug targets still exists. Historically, RNA polymerase activity has been ... More
Quantification of mRNA using real-time RT-PCR.
Authors:Nolan T, Hands RE, Bustin SA
Journal:Nat Protoc
PubMed ID:17406449
'The real-time reverse transcription polymerase chain reaction (RT-qPCR) addresses the evident requirement for quantitative data analysis in molecular medicine, biotechnology, microbiology and diagnostics and has become the method of choice for the quantification of mRNA. Although it is often described as a "gold" standard, it is far from being a ... More
A systemic small RNA signaling system in plants.
Authors:Yoo BC, Kragler F, Varkonyi-Gasic E, Haywood V, Archer-Evans S, Lee YM, Lough TJ, Lucas WJ
Journal:Plant Cell
PubMed ID:15258266
'Systemic translocation of RNA exerts non-cell-autonomous control over plant development and defense. Long-distance delivery of mRNA has been proven, but transport of small interfering RNA and microRNA remains to be demonstrated. Analyses performed on phloem sap collected from a range of plants identified populations of small RNA species. The dynamic ... More
Fibrinogen induces IL-8 synthesis in human neutrophils stimulated with formyl-methionyl-leucyl-phenylalanine or leukotriene B(4).
Authors:Kuhns DB, Nelson EL, Alvord WG, Gallin JI
Journal:J Immunol
PubMed ID:11509634
'Human exudative neutrophils have greatly increased stores of the neutrophil chemoattractant IL-8 compared with peripheral blood cells, but the mechanism for the increase is not defined. In this report, we show that treatment of peripheral blood neutrophils with the chemotactic peptide fMLP or with leukotriene B(4) or fibrinogen results in ... More
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