Blasticidin S HCl, powder

Citations & References (24)

Gibco™

Blasticidin S HCl, powder

ブラストチジンSは、ストレプトミセスグリセオクロモ遺伝子から単離されたヌクレオチド抗生物質です。細菌や真核生物におけるタンパク質合成の強力な阻害剤であり、真菌類、線虫、腫瘍細胞にも有効です。ブラストチジンSは、放出因子によって誘発されるペプチジル-tRNAの加水分解を阻害することで作用し、ペプチド結合形成を阻害します。哺乳類細胞と細菌細胞の両方で選択剤として使用されます詳細を見る

製品番号（カタログ番号）	数量
R21001	50 mg

製品番号（カタログ番号） R21001

価格（JPY）

47,300

お問い合わせください ›

50 mg

ブラストチジンSは、ストレプトミセスグリセオクロモ遺伝子から単離されたヌクレオチド抗生物質です。細菌や真核生物におけるタンパク質合成の強力な阻害剤であり、真菌類、線虫、腫瘍細胞にも有効です。ブラストチジンSは、放出因子によって誘発されるペプチジル-tRNAの加水分解を阻害することで作用し、ペプチド結合形成を阻害します。哺乳類細胞と細菌細胞の両方で選択剤として使用されます。推奨される使用濃度は、細胞株に応じて1～30 µg/mL、細菌の選択には25–100 µg/mLです。細胞死は急速に起こり、ブラスティチジン耐性の安定した哺乳類細胞株は1週間未満で生成できます。

ブラストサイジンSに対する耐性は、セレウス菌K55-Sおよびアスペルギルステルレウスからそれぞれ分離されたBSRおよびBSDによりもたらされます。BSR耐性遺伝子はブラストサイジンSデアミナーゼをエンコードします。これはブラストサイジンSのdeaminohydroxyblasticidin Sへの変換を触媒します。Deaminohydroxyblasticidin Sは生物学的に不活性なブラストサイジンSの誘導体であり、原核生物と真核生物のいずれのリボソームにも作用または阻害しません。また、BSD耐性遺伝子はブラストサイジンSデアミナーゼをエンコードし、これはBSRデアミナーゼと同様の反応を触媒します。

細菌の選択を目的とした場合、LB培地の塩含有量は低く（<90 mM）、pHは7.0を超えないようにして、ブラストサイジンSの活性を維持する必要があります。耐性のない細胞を死滅させるためにもっとも効率の低いブラストサイジンS濃度を決定するために、殺菌曲線を推奨します。

アプリケーション
哺乳類細胞、大腸菌、酵母のブラストサイジン選択に関するプロトコルの詳細をご覧ください。

研究用途にのみご使用ください。診断目的には使用できません。

細胞タイプEukaryotic Cells, Prokaryotic Cells

色White, off-white

濃度50 to 100 μg/mL

培養タイプMammalian Cell Culture, Insect Cell Culture

使用対象（アプリケーション）Bacterial Selection

製品ラインGibco

数量50 mg

品質保持期間Lot Dependent

形状Powder

製品タイプAntibiotic

無菌性Sterile

添加剤ありHEPES

Unit SizeEach

組成および保存条件

Storage conditions: -5 to -30°C
Shipping conditions: room temperature
Shelf life: Lot Dependent

よくあるご質問（FAQ）

Which of your antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for stable selection in mammalian cells?

All of our antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for making multiple stable cell lines. However, kill curves will need to be performed for each combination of antibiotics since sensitivity to a given antibiotic tends to increase when combined with other antibiotics.

What are the recommended concentrations of antibiotics to use for selection in prokaryotes and eukaryotes?

For best results, optimal concentrations for selection should be determined empirically in each unique experiment through dose response curves. However, to get a general idea of concentrations that have worked for individual cell types, please click on the following url: http://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/selection.html or type in “Selection Antibiotics” into our main search on www.thermofisher.com.

What is the mode of action on the following antibiotics: Blasticidin, Geneticin (G418), Hygromycin, and Zeocin?

Blasticidin: Nucleoside Inhibits protein synthesis in prokaryotic and eukaryotic cells by interfering with peptidyl transfer reaction of protein synthesis, causing early termination of translation.

Geneticin (G418): Aminoglycoside Blocks protein synthesis in mammalian cells by interfering with ribosomal function.

Hygromycin: Aminocyclitol Inhibits protein synthesis by disrupting translocation and promoting mistranslation.

Zeocin: Intercalates with DNA and cleaves it.

What is the optimal pH of low salt LB for LB + blasticidin plates?

We recommend a pH of 7 or less and half the normal amount of NaCl in your LB media or plates.

See the following paper for details on optimal conditions: Yamaguchi et al (1965) Inhibition of Protein Synthesis by Blasticidin S. Journal of Biochemistry (Tokyo) Volume 57: pp 667-677.

How long can Blasticidin be stored at 4 degrees C after thawing? Does the unused portion have to be discarded after thawing?

Blasticidin is stable for 6 months when stored at 4 degrees C. Discard remaining material after this time.

View all Blasticidin S HCl, powder FAQs

引用および参考文献 (24)

引用および参考文献

Abstract

Suppression of Ras-mediated tumorigenicity and metastasis through inhibition of the Met receptor tyrosine kinase.

Authors: Furge K A; Kiewlich D; Le P; Vo M N; Faure M; Howlett A R; Lipson K E; Woude G F; Webb C P;

Journal:Proc Natl Acad Sci U S A

PubMed ID:11535809

'Mutations in the Ras family of GTP binding proteins represent one of the most frequently observed genetic alterations in human cancers. We and others have recently demonstrated that expression of Met, the tyrosine kinase receptor for hepatocyte growth factor/scatter factor (HGF/SF), is significantly up-regulated in Ras-transformed cells. Because HGF/SF-Met signaling ... More

Structural basis of G protein specificity of human endothelin receptors. A study with endothelinA/B chimeras.

Authors: Takagi Y; Ninomiya H; Sakamoto A; Miwa S; Masaki T;

Journal:J Biol Chem

PubMed ID:7730310

'The endothelin (ET) family of peptides acts via two subtypes of guanine nucleotide-binding regulatory protein (G protein)-coupled receptors termed ETA and ETB. ET-1 stimulated cAMP formation in Chinese hamster ovary (CHO) cells stably expressing human wild-type ETA (CHO/hETA cells) while it inhibited cAMP formation in CHO cells expressing human wild-type ... More

West Nile virus discriminates between DC-SIGN and DC-SIGNR for cellular attachment and infection.

Authors:Davis CW, Nguyen HY, Hanna SL, Sánchez MD, Doms RW, Pierson TC,

Journal:J Virol

PubMed ID:16415006

'The C-type lectins DC-SIGN and DC-SIGNR bind mannose-rich glycans with high affinity. In vitro, cells expressing these attachment factors efficiently capture, and are infected by, a diverse array of appropriately glycosylated pathogens, including dengue virus. In this study, we investigated whether these lectins could enhance cellular infection by West Nile ... More

Presenilin 1 mutations activate gamma 42-secretase but reciprocally inhibit epsilon-secretase cleavage of amyloid precursor protein (APP) and S3-cleavage of notch.

Authors: Chen Fusheng; Gu YongJun; Hasegawa Hiroshi; Ruan Xueying; Arawaka Shigeki; Fraser Paul; Westaway David; Mount Howard; St George-Hyslop Peter;

Journal:J Biol Chem

PubMed ID:12119298

'The presenilin 1 (PS1) and presenilin 2 (PS2) proteins are necessary for proteolytic cleavage of the amyloid precursor protein (APP) within its transmembrane domain. One of these cleavage events (termed gamma-secretase) generates the C-terminal end of the Abeta-peptide by proteolysis near residue 710 or 712 of APP(770). Another event (termed ... More

Pattern of genes influenced by conditional expression of the transcription factors HNF6, HNF4alpha and HNF1beta in a pancreatic beta-cell line.

Authors:Thomas H, Senkel S, Erdmann S, Arndt T, Turan G, Klein-Hitpass L, Ryffel GU,

Journal:Nucleic Acids Res

PubMed ID:15520459

'Using the rat insulinoma cell line INS-1 we generated beta-cell clones that are most efficient for gene transfer, as they contain an FRT site for Flp recombinase-mediated, site-directed integration of a single copy transgene. Therefore, the gene-of-interest can be introduced by DNA transfection without the need to select individual cell ... More

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