Rhodamine 123, 25 mg

Citations & References (715)

Invitrogen™

Rhodamine 123, 25 mg

ローダミン123は細胞透過性の陽イオン性グリーン蛍光色素で、細胞毒性の影響を受けずに活性ミトコンドリアによって容易に隔離されます。この製品は、アポトーシス細胞集団におけるミトコンドリア膜電位の測定に使用されています。詳細を見る

製品番号（カタログ番号）	数量
R302	25 mg

製品番号（カタログ番号） R302

価格（JPY）

26,200

お問い合わせください ›

25 mg

ローダミン123は細胞透過性の陽イオン性グリーン蛍光色素で、細胞毒性の影響を受けずに活性ミトコンドリアによって容易に隔離されます。この製品は、アポトーシス細胞集団におけるミトコンドリア膜電位の測定に使用されています。

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光

数量25 mg

出荷条件室温

細胞内局在ミトコンドリア

色Green

使用対象 (装置)蛍光顕微鏡

製品タイプRhodamine 123

Unit SizeEach

組成および保存条件

フリーザー(-5°C∼-30°C)に保存し、遮光してください。

よくあるご質問（FAQ）

What is the excitation and emission wavelength for rhodamine?

Rhodamine is a generic term for a wide variety of cationic dyes whose fluorescence emission can range from green, orange to red. The table below lists the excitation and emission maxima (nm), as well as molar extinction coefficients (“EC”; cm^-1 M^-1), for various rhodamine dyes (data derived with dye dissolved in methanol).

Dye	Excitation	Emission	EC
Rhodamine B	568	583	88,000
Rhodamine 123	507	529	101,000
Rhodamine 110	499	521	92,000
Rhodamine 6G	528	551	105,000
XRITC	572	596	92,000

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between fast and slow-response membrane potential probes?

Molecules that change their structure in response to the surrounding electric field can function as fast-response probes for the detection of transient (millisecond) potential changes. Slow-response dyes function by entering depolarized cells and binding to proteins or membranes. Increased depolarization results in additional dye influx and an increase in fluorescence, while hyperpolarization is indicated by a decrease in fluorescence. Fast-response probes are commonly used to image electrical activity from intact heart tissues or measure membrane potential changes in response to pharmacological stimuli. Slow-responding probes are often used to explore mitochondrial function and cell viability.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of membrane potential indicators do you offer and how should I choose one for my experiment?

A membrane potential indicator selection guide can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (715)

引用および参考文献

Abstract

The mitochondrial death/life regulator in apoptosis and necrosis.

Authors:Kroemer G,Dallaporta B,Resche-Rigon M

Journal:Annual review of physiology

PubMed ID:9558479

Both physiological cell death (apoptosis) and, in some cases, accidental cell death (necrosis) involve a two-step process. At a first level, numerous physiological and some pathological stimuli trigger an increase in mitochondrial membrane permeability. The mitochondria release apoptogenic factors through the outer membrane and dissipate the electrochemical gradient of the ... More

2,4 Dinitrophenol-uncoupling effect on delta psi in living hepatocytes depends on reducing-equivalent supply.

Authors:Sibille B, Ronot X, Filippi C, Nogueira V, Keriel C, Leverve X

Journal:Cytometry

PubMed ID:9627223

Mitochondrial uncouplers, such as 2,4 dinitrophenol (DNP), increase the cellular respiration by decreasing mitochondrial membrane potential (delta psi). We show that this respiratory effect can be transient or even prevented in isolated liver cells depending on the exogenous substrate used (dihydroxyacetone vs. octanoate or proline). Moreover the decrease in ATP/ADP ... More

Is rhodamine 123 an appropriate fluorescent probe to assess P-glycoprotein mediated multidrug resistance in vinblastine-resistant CHO cells?

Journal:Anal Cell Pathol

PubMed ID:9354229

Cellular drug resistance, which involves several mechanisms such as P-glycoprotein (P-gp) overexpression, kinetic and metabolic quiescence, or the increase in the intracellular levels of glutathione, limits the effectiveness of cancer treatment. It has been reported that functional assessment of the cationic dye rhodamin 123 (Rho123) efflux reveals accurately the drug-resistant ... More

Human epidermal cells progressively lose their cardiolipins during ageing without change in mitochondrial transmembrane potential.

Journal:Mech Ageing Dev

PubMed ID:7745994

Mitochondria dysfunction is considered to be a major cause of the modifications that occur during cell ageing. For this reason, cardiolipin, a suitable marker of the chondriome, as well as the mitochondrial transmembrane potential were examined in keratinocytes obtained from 9- to 75-year-old women. The study was carried out by ... More

Flow cytometric functional analysis of multidrug resistance by Fluo-3: a comparison with rhodamine-123.

Authors:Koizumi S, Konishi M, Ichihara T, Wada H, Matsukawa H, Goi K, Mizutani S

Journal:Eur J Cancer

PubMed ID:7488425

Using four cell lines including drug-sensitive K562/Parent cells, P-glycoprotein (Pgp)-mediated multidrug resistant (MDR) K562/VCR, K562/ADR and revertant K562/ADR-R cells, two fluorescent agents, Fluo-3 and rhodamine-123 (Rh-123), were compared as indicators in a functional assay of MDR. Cells were incubated with 4 microM Fluo-3 or 1 microM Rh-123 for 45 min ... More

View all Rhodamine 123, 25 mg citations