EZQ™ Protein Quantitation Kit
Product Image
Citations & References (7)
Invitrogen™

EZQ™ Protein Quantitation Kit

EZQ™タンパク質定量キットでは、ゲル電気泳動用に調製されたタンパク質サンプルの迅速な定量化をより簡単に行うための蛍光ベースのタンパク質アッセイを使用できます。アッセイは、界面活性剤、尿素、還元剤が存在する状態で実施できます—用意されたペーパーの上に1µLのタンパク質サンプルを落とすだけで、特許取得済みの蛍光染料で染色し、蛍光を測定できます。サンプルは、標準曲線と比較して定量化できます詳細を見る
テクニカルサポートオーダーサポート
製品番号（カタログ番号）数量
R332002000アッセイ
製品番号（カタログ番号） R33200
価格（JPY）
123,200
Each
お問い合わせください ›
数量:
2000アッセイ
一括またはカスタム形式をリクエストする
EZQ™タンパク質定量キットでは、ゲル電気泳動用に調製されたタンパク質サンプルの迅速な定量化をより簡単に行うための蛍光ベースのタンパク質アッセイを使用できます。アッセイは、界面活性剤、尿素、還元剤が存在する状態で実施できます—用意されたペーパーの上に1µLのタンパク質サンプルを落とすだけで、特許取得済みの蛍光染料で染色し、蛍光を測定できます。サンプルは、標準曲線と比較して定量化できます。汎用性を高めるために、マイクロプレートリーダーやレーザースキャナーでサンプルを簡単に定量できるように特別設計された96ウェルマイクロプレートを提供しています。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
アッセイタンパク質定量アッセイ
使用対象（アプリケーション）タンパク質定量アッセイ
使用対象 (装置)マイクロプレートリーダー
製品ラインEZQ
製品タイプProtein Quantitation Assay
数量2000アッセイ
出荷条件室温
保存要件室温保存。遮光。
対応可能対象2000アッセイ
検出法蛍光
Unit SizeEach
組成および保存条件
室温で保存し、光から保護します。

よくあるご質問（FAQ）

I am using the EZQ Protein Quantitation Kit and the filter paper has curled up during drying. What should I do?

It is not unusual for the filter paper to bend and warp on drying. It is very important that the paper be flat when scanning or the signal will be uneven and give very inaccurate quantitation values. If this is a problem, wet the paper in water and scan it while it is wet.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I am using the EZQ Protein Quantitation Kit and after staining, I notice some large spots or marks on the paper. Why is this?

Protein on the skin (e.g. keratin) will transfer to the filter paper and be stained with the EZQ Protein Quantitation Reagent. We recommend handling the filter paper with tweezers and cleaning the staining dish and tweezers before use to minimize marks.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I spotted my protein samples onto the EZQ protein assay filter paper and I do not have time to complete the assay? Can I stop the procedure and finish later?

Yes, once the samples have dried onto the filter paper, the filter paper can be stored and stained at a later date. After the staining procedure is complete, the signal is very stable, so the paper can be dried again and scanned at a later date as well. The paper can be scanned dry or after dipping it in water. If storing the paper before staining, we recommend storing it in a plastic bag to prevent contamination that could affect the staining pattern.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I ran out of filter paper for the EZQ Protein Quantitation Kit. Can I just cut any filter paper to fit inside the microplate?

No. The filter paper that is supplied with the EZQ Protein Quantitation Kit is a very specific filter paper and the identity is proprietary. Most lab filter papers will not provide similar results as the paper provided in the kit, so substitutions are not recommended.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

My buffer or components of my buffer are not listed in the compatibility table for my protein assay. What should I do?

You can test the tolerance of the assay for your specific buffer formulation. For in-house generated compatibility information, substances were considered compatible at the indicated concentration in the Standard Test Tube Protocol (found in the manual for each protein assay) if the error in protein concentration estimation caused by the presence of the substance was less than or equal to 10%. The substances were tested using WR prepared immediately before each experiment. Blank-corrected 562nm absorbance measurements (for a 1000µg/mL BSA standard + substance) were compared to the net 562nm measurements of the same standard prepared in 0.9% saline.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all EZQ™ Protein Quantitation Kit FAQs

引用および参考文献 (7)

引用および参考文献
Abstract
Induced paternal effects mimic cytoplasmic incompatibility in Drosophila.
Authors:Clark ME, Heath BD, Anderson CL, Karr TL
Journal:Genetics
PubMed ID:16489228
'Wolbachia is an intracellular microbe found in a wide diversity of arthropod and filarial nematode hosts. In arthropods these common bacteria are reproductive parasites that manipulate central elements of their host''s reproduction to increase their own maternal transmission in one of several ways. Cytoplasmic incompatibility (CI) is one such manipulation ... More
A novel approach to tag and identify geranylgeranylated proteins.
Authors:Chan LN, Hart C, Guo L, Nyberg T, Davies BS, Fong LG, Young SG, Agnew BJ, Tamanoi F,
Journal:Electrophoresis
PubMed ID:19784953
A recently developed proteomic strategy, the
Gamma-secretase is a functional component of phagosomes.
Authors:Jutras I, Laplante A, Boulais J, Brunet S, Thinakaran G, Desjardins M
Journal:J Biol Chem
PubMed ID:16103123
Gamma-secretase is a high molecular mass protein complex that catalyzes the intramembrane cleavage of its protein substrates. Two proteins involved in phagocytosis, CD44 and the low density lipoprotein receptor-related protein, are gamma-secretase substrates, suggesting that this complex might regulate some aspects of phagocytosis. Our results indicate that the four components ... More
Mechanism of intramembrane proteolysis investigated with purified rhomboid proteases.
Authors:Lemberg MK, Menendez J, Misik A, Garcia M, Koth CM, Freeman M
Journal:EMBO J
PubMed ID:15616571
Intramembrane proteases have the unusual property of cleaving peptide bonds within the lipid bilayer, an environment not obviously suited to a water-requiring hydrolysis reaction. These enzymes include site-2 protease, gamma-secretase/presenilin, signal peptide peptidase and the rhomboids, and they have a wide range of cellular functions. All have multiple transmembrane domains ... More
A rapid solid-phase fluorescence-based protein assay for quantitation of protein electrophoresis samples containing detergents, chaotropes, dyes, and reducing agents.
Authors:Agnew BJ, Murray D, Patton WF
Journal:Electrophoresis
PubMed ID:15300765
A new solid-phase, fluorescence-based protein assay was developed that quantifies proteins in the presence of detergents, urea and reducing agents (one-dimensional sodium dodecyl sulfate (1-D SDS) lysis buffers and urea isoelectric focusing (IEF) buffers). A specially designed 96-well microplate facilitates application of protein samples to the assay paper and allows ... More
View all EZQ™ Protein Quantitation Kit citations