ReadyProbes™ F-Actin Labeling Reagents
Invitrogen™

ReadyProbes™ F-Actin Labeling Reagents

ActinRed™ and ActinGreen™ ReadyProbes™ reagents assist with bright, selective F-actin labeling in fixed cells and tissues. The ready-to-use dropper or dual-use bottle enables dropwise dispensing or direct pipetting for fast, flexible cytoskeleton imaging.
製品番号(カタログ番号)染色剤タイプ励起波長域
R37110Alexa Fluor™ 488495/518
R37112TRITC(テトラメチルローダミンイソチオシアン酸塩)540/565
R37178Alexa Fluor™ 568580/603
R37179Alexa Fluor™ Plus 647658/675
製品番号(カタログ番号) R37110
価格(JPY)
44,200
Each
染色剤タイプ:
Alexa Fluor™ 488
励起波長域:
495/518

ActinGreen 488 ReadyProbes Reagent is a selective, high-affinity F-actin probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor 488 dye. ActinGreen 488 reagent is a room temperature-stable solution of Alexa Fluor 488 ActinGreen (Cat. No. A12379) packaged in a dropper bottle. ActinRed 555 ReadyProbes Reagent is a comparable F-actin stain conjugated to the red-orange fluorescent dye tetramethylrhodamine (TRITC). ActinRed 555 reagent is a room temperature-stable solution of Rhodamine F-actin probe (Cat. No. R415) packaged in a dropper bottle.

ActinRed 568 ReadyProbes Reagent is our high-affinity F-actin probe conjugated to our bright, red-fluorescent Alexa Fluor 568 dye. This dye is packaged in an easy to use dropper-optional bottle, allowing end-users to choose either to pipet out of the tube using the classic cap, or utilize the dropper bottle tip for typical ReadyProbes workflows.

ActinRed 647 ReadyProbes Reagent leverages our high-affinity F-actin probe conjugated to our bright Far-Red Alexa Fluor 647 Plus dye, offering enhanced brightness vs. classic Alexa Fluor 647 F-actin probe conjugates, in an easy to use format. ActinRed 647 ReadyProbes Reagent should be stored from 2-8°C for long-term, and also offers the option to leverage pipetting, if desired, via the dropper-optional cap.

These ready-to-use solutions combine superior brightness with exceptional F-actin staining. ReadyProbes actin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals and low non-specific binding.

研究用にのみ使用できます。診断用には使用いただけません。

仕様
細胞透過性Fixed Cells
Green
供給タイプAdd 2 drops (80 μL) per 1 mL of medium
概要ActinGreen™ 488 ReadyProbes™ Reagent (AlexaFluor™ 488 phalloidin)
検出法蛍光
染色剤タイプAlexa Fluor™ 488
発光可視
励起498 nm
励起波長域495/518
使用対象(アプリケーション)Fixed Cell Imaging, High Content Screening, Fluorescence Microscopy
使用対象 (装置)共焦点顕微鏡、Floid™細胞イメージングシステム、蛍光顕微鏡、フローサイトメーター、Floid™細胞イメージングシステム
形状液体
フォーマットDropper bottles
テスト数75 Tests
製品ラインActinGreen、ReadyProbes
数量2ドロッパーボトル
反応性F-actin
ターゲットF-actin Cytoskeleton
技術蛍光強度
標識タイプFluorescent Dye
製品タイプファロイジン
Sub Cellular Localization細胞骨格
Unit SizeEach
組成および保存条件
2 x 2.5 mLドロッパーボトル

≤25°Cで保管してください。

よくあるご質問(FAQ)

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (40)

引用および参考文献
Abstract
Iridium oxide nanotube electrodes for sensitive and prolonged intracellular measurement of action potentials.
Authors:Lin ZC, Xie C, Osakada Y, Cui Y, Cui B,
Journal:
PubMed ID:24487777
'Intracellular recording of action potentials is important to understand electrically-excitable cells. Recently, vertical nanoelectrodes have been developed to achieve highly sensitive, minimally invasive and large-scale intracellular recording. It has been demonstrated that the vertical geometry is crucial for the enhanced signal detection. Here we develop nanoelectrodes of a new geometry, ... More
The cellular and proteomic response of primary and immortalized murine Kupffer cells following immune stimulation diverges from that of monocyte-derived macrophages.
Authors:Tweedell R, Tao D, Dinglasan RR,
Journal:
PubMed ID:25266554
Kupffer cells (KCs) are the first line of defense in the liver against pathogens, yet several microbes successfully target the liver, bypass immune surveillance, and effectively develop in this tissue. Our current, albeit poor, understanding of KC-pathogen interactions has been largely achieved through the study of primary cells, requiring isolation ... More
Distinct adipogenic differentiation phenotypes of human umbilical cord mesenchymal cells dependent on adipogenic conditions.
Authors:Saben J, Thakali KM, Lindsey FE, Zhong Y, Badger TM, Andres A, Shankar K,
Journal:
PubMed ID:24951473
The umbilical cord (UC) matrix is a source of multipotent mesenchymal stem cells (MSCs) that have adipogenic potential and thus can be a model to study adipogenesis. However, existing variability in adipocytic differentiation outcomes may be due to discrepancies in methods utilized for adipogenic differentiation. Additionally, functional characterization of UCMSCs ... More
CD8 T Cells Use IFN-? To Protect against the Lethal Effects of a Respiratory Poxvirus Infection.
Authors:Goulding J, Abboud G, Tahiliani V, Desai P, Hutchinson TE, Salek-Ardakani S,
Journal:
PubMed ID:24748494
CD8 T cells are a key component of immunity to many viral infections. They achieve this through using an array of effector mechanisms, but precisely which component/s are required for protection against a respiratory orthopox virus infection remains unclear. Using a model of respiratory vaccinia virus infection in mice, we ... More
An iron-regulated and glycosylation-dependent proteasomal degradation pathway for the plasma membrane metal transporter ZIP14.
Authors:Zhao N, Zhang AS, Worthen C, Knutson MD, Enns CA,
Journal:
PubMed ID:24927598
Protein degradation is instrumental in regulating cellular function. Plasma membrane proteins targeted for degradation are internalized and sorted to multivesicular bodies, which fuse with lysosomes, where they are degraded. ZIP14 is a newly identified iron transporter with multitransmembrane domains. In an attempt to dissect the molecular mechanisms by which iron ... More