Invitrogen™

SYTOX AADvanced™ Ready Flow™ Reagent

SYTOX AADvanced Ready Flow試薬は、フローサイトメトリーで死細胞を検出するための高親和性核酸染色試薬です。色素の最大励起／発光波長は546/647 nmです。•室温で安定しています• 便利なすぐに使えるフォーマット —詳細を見る

製品番号（カタログ番号）	数量
R37173	120 Reactions

製品番号（カタログ番号） R37173

価格（JPY）

26,800

온라인 행사

Ends: 26-Jun-2026

44,800

割引額 18,000 (40%)

120 Reactions

SYTOX AADvanced Ready Flow試薬は、フローサイトメトリーで死細胞を検出するための高親和性核酸染色試薬です。色素の最大励起／発光波長は546/647 nmです。

•室温で安定しています
• 便利なすぐに使えるフォーマット — 希釈、計量、ピペットでの分注が不要です
• 迅速な取り込みと低変動係数（CV）

SYTOX AADvanced Ready Flow試薬は、すぐに使える、サンプルに直接使用可能な死細胞染色剤です。DNAに結合したときに赤色蛍光を発します。ドロッパーボトルから直接、1 x 10⁶個の細胞に2滴加え、インキュベートして分析します。

SYTOX AADvanced Ready Flow試薬色素は、スペクトル的には7-AADに似ていますが、迅速な取り込み速度と比較的低いCV値を有しています。7-AADよりも効率的に細胞に浸透し、生細胞と死細胞の分離を向上させます。

研究用途にのみご使用ください。診断目的には使用できません。

細胞透過性Impermeant

概要SYTOX AADvanced™ Ready Flow™ Reagent

検出法蛍光

染色剤タイプCell-Impermeant

フォーマットBottle(s)

数量120 Reactions

出荷条件室温

溶解性DMSO（ジメチルスルホキシド）

細胞内局在Nucleus

Emission546/647

Excitation Wavelength Range546 nm

使用対象（アプリケーション）Viability Assay

使用対象 (装置)Flow Cytometer

製品ラインAADvanced、Ready Flow

製品タイプ試薬

Unit SizeEach

組成および保存条件

ドロッパーボトル2本、室温で保管してください

よくあるご質問（FAQ）

I am using SYTOX AAdvanced as a dead cell stain, but all of my cells are labeling even though I am certain that they are supposed to be alive. These are adherent cells that I have trypsinized. Why am I getting false-dead signals?

SYTOX AAdvanced labels only dead cells because it is a cell impermeant dye. The dye can only enter cells that have a compromised plasma membrane. Trypsinization may cause temporary disruption of the plasma membrane, sufficient to allow staining with a cell impermeant dye. You can reduce the “false-dead” problem by either reducing the amount of trypsin and/or reduce the incubation time for trypsinization or use a gentler dissociation reagent such as TrypLE Express, TrypLESelect reagents, or Versene. After trypsinization, wash well, and if possible, allow a recovery time in normal culture media before staining with any of the SYTOX dyes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I would like to label only 100 µL of sample of the same cell density using a Ready Flow product. The manual notes 2 drops per 1 mL (1 X 10E6 cells/mL). May I scale down for smaller volumes?

Yes, you may scale up or down as needed, but we recommend keeping the cell density the same. There are 41 µL per drop, so 2 drops is 82 µL (for the 1 mL of sample). You need to only scale down for this 100 µL volume, which would be 8.2 µL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the concentration of dye/reagent in the Ready Flow products?

The amount of dye or reagent in the Ready Flow products is proprietary. If you need to consider the exact amount of dye or reagent for your experiment, each of the Ready Flow reagents is available as a standalone product.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.