Can I use ReadyProbes Mouse-on-Mouse IgG Blocking Solution (30X) on rat tissues?
Due to some cross-reactivity of anti-mouse antibodies to rat antibodies, using ReadyProbes Mouse-on-Mouse IgG Blocking Solution (30X) on rat tissues may offer some level of blocking, but it may not be sufficient. For rat tissues, ideally you should use a rat-on-rat blocking.
Do I need to use ReadyProbes Mouse-on-Mouse IgG Blocking Solution (30X) on cultured mouse cells?
Assuming that the cultured mouse cells are not expressing mouse antibodies, ReadyProbes Mouse-on-Mouse IgG Blocking Solution (30X) is not needed for cultured mouse cells.