Can ReadyProbes Endogenous HRP and AP Blocking Solution (1X) be used on cell or tissue lysates?
We do not recommend using ReadyProbes Endogenous HRP and AP Blocking Solution (1X) on cell or tissue lysates. The ReadyProbes Endogenous HRP and AP Blocking Solution (1X) must be removed prior to the application of any HRP- or AP-conjugated antibody/streptavidin. If analyzing the lysates directly, the ReadyProbes Endogenous HRP and AP Blocking Solution (1X) cannot be removed from the lysate.