Does the ReadyProbes Tissue Autofluorescence Quenching Kit quench all autofluorescence?
No. The ReadyProbes Tissue Autofluorescence Quenching Kit minimizes autofluorescence created by aldehyde fixation, red blood cell autofluorescence, and autofluorescence of collagen and elastin. It will not quench natural pigments or other autofluorescent entities.
Will the ReadyProbes Tissue Autofluorescence Quenching Kit still work if I messed up the order of addition, i.e., if I mixed Component A and Component C (instead of Component B) and then added Component B?
For the ReadyProbes Tissue Autofluorescence Quenching Kit to work, the components need to be mixed in the recommended order. Component A needs to be mixed with Component B, followed by mixing in Component C.
With the ReadyProbes Tissue Autofluorescence Quenching Kit, once I mix Components A, B, and C together, can I store this mixture in the refrigerator or freezer for later use?
Once the 3 components are mixed together, we recommend using this mixture within 1 hour. The mixture should not be stored beyond 1 hour.