What is the excitation and emission wavelength for rhodamine?
Rhodamine is a generic term for a wide variety of cationic dyes whose fluorescence emission can range from green, orange to red. The table below lists the excitation and emission maxima (nm), as well as molar extinction coefficients (EC; cm-1 M-1), for various rhodamine dyes (data derived with dye dissolved in methanol).
Evolution of peptides that modulate the spectral qualities of bound, small-molecule fluorophores.
Authors:Rozinov MN, Nolan GP
Journal:Chem Biol
PubMed ID:9862799
'BACKGROUND: Fluorophore dyes are used extensively in biomedical research to sensitively assay cellular constituents and physiology. We have created, as proof of principle, fluorophore dye binding peptides that could have applications in fluorescent dye-based approaches in vitro and in vivo. RESULTS: A panel of Texas red, Rhodamine red, Oregon green ... More
Spectroscopic investigation of a FRET molecular beacon containing two fluorophores for probing DNA/RNA sequences.
Authors:Jockusch S, Martí AA, Turro NJ, Li Z, Li X, Ju J, Stevens N, Akins DL
Journal:Photochem Photobiol Sci
PubMed ID:16685327
'We report the design, synthesis, and characterization of a molecular beacon (MB) consisting of two fluorescent dyes (Alexa 488 and RedX) for DNA and RNA analysis. In the absence of the target DNA or RNA the MB is in its stem-closed form and shows efficient energy transfer from the donor ... More
Delivery of oligonucleotides into mammalian cells by anionic peptides: comparison between monomeric and dimeric peptides.
Authors:Freulon I, Roche AC, Monsigny M, Mayer R
Journal:Biochem J
PubMed ID:11237872
'The use of antisense oligonucleotides as putative therapeutic agents is limited by their poor delivery into the cytosol and/or the nucleus because they are not able to efficiently cross lipid bilayers. To circumvent this pitfall, anionic amphipathic peptides derived from the influenza virus fusogenic peptide have been used to destabilize ... More
Chemistry of sulforhodamine--amine conjugates.
Authors:Corrie JE, Davis CT, Eccleston JF
Journal:Bioconjug Chem
PubMed ID:11312679
Commercially-available sulforhodamine sulfonyl chlorides contain two isomeric monosulfonyl chlorides. Conjugates of these isomers with amines have different properties because the sulfonamide formed from one isomer can undergo ring-closure to a colorless sultam. This chemistry has been examined for a model conjugate with methylamine and for a bioconjugate with 2'(3')-O-[N-(2-aminoethyl)carbamoyl]ATP. The ... More
Imaging of the fluorescence spectrum of a single fluorescent molecule by prism-based spectroscopy.
Authors:Suzuki Y, Tani T, Sutoh K, Kamimura S
Journal:FEBS Lett
PubMed ID:11852087
We have devised a novel method to visualize the fluorescence spectrum of a single fluorescent molecule using prism-based spectroscopy. Equipping a total internal reflection microscope with a newly designed wedge prism, we obtained a spectral image of a single rhodamine red molecule attached to an essential light chain of myosin. ... More