293FT Cell Line

Citations & References (3)

Invitrogen™

293FT Cell Line

293FT 細胞株は、ViraPower™ Lentiviral Expression System を使用した高力価レンチウイルスの生成に最適です。293FT 細胞株は詳細を見る

製品番号（カタログ番号）	数量
R70007	1 x 10^7細胞

製品番号（カタログ番号） R70007

価格（JPY）

104,100

お問い合わせください ›

1 x 10^7細胞

293FT 細胞株は、ViraPower™ Lentiviral Expression System を使用した高力価レンチウイルスの生成に最適です。293FT 細胞株は、SV40 ラージ T 抗原で形質転換されたヒト胚性腎細胞から得られた、急速に増殖し、高度にトランスフェクト可能なクローンアイソレートです。ViraPower™ 発現ベクターと ViraPower™ Lentiviral Packaging Mix を 293FT 細胞に同時トランスフェクトすると、パッケージングに必要な高レベルのウイルス RNA と gag⁄pol および rev タンパク質が生成されます。

利点
• 急速な増殖
•高いトランスフェクト性。
•高いウイルス力価を生成します。
•非常に高レベルなタンパク質の生成が可能です。

主な特徴
•SV40 ラージ T 抗原の存在により、SV40 由来のベクターから非常に高レベルのタンパク質を発現させることができます。実際、他の 293 細胞や従来から使用されている T抗原で形質転換された COS 細胞よりも高レベルです。

キットには、90％完全培地 1 mLと 10％ DMSO 内の凍結保存用ストックとして提供される 293FT 細胞株が含まれます。
•

研究用途にのみご使用ください。あらゆる治療もしくは診断用には使用できません。

推奨培地SFM (Serum Free Medium)

セル数1 x 10⁷

製品ラインViraPower

数量1 x 10^7細胞

細胞株293FT

Unit SizeEach

組成および保存条件

• 293FT 細胞株（液体窒素で保存）

よくあるご質問（FAQ）

Do 293FT cells lift off the plate during lentivirus production?

If 293FT cells detach shortly after transfection (4 hours to overnight):

- This may be a sign of Lipofectamine 2000 toxicity. Cells may have been plated too sparsely prior to transfection.
- The cells may not have been handled gently enough (these cells have a tendency to lift off easily).
- The cells may have been kept at room temperature for too long.

If cells detach 48 to 72 hours post-transfection:
- If the cells lift off in large sheets, this may be a sign of lentivirus production.

What are syncytia?

Syncytia are large multi-nucleated cells that result from VSV-G-induced fusion with neighboring 293FT producer cells. Syncytia production is indicative of high transfection efficiency and lentivirus production. Keep in mind, though, that the absence of syncytia does not mean that virus will not be produced.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Why do I have to culture 293FT cells under Geneticin antibiotic selection?

The 293FT cell line stably expresses the SV40 large T antigen from the pCMVSPORT6Tag.neo plasmid that contains the neomycin resistance marker. In order to maintain the plasmid/phenotype, the cells have to be routinely cultured in medium containing Geneticin (G418) antibiotic at a concentration of 500 µg/mL.

Do you recommend a specific FBS for culturing 293FT cells? Which plastic plates do you recommend?

We use Mycoplasma-tested Gibco FBS (Cat. No. 16000-044). We have observed that when 293FT cells are cultured in the presence of this FBS following the instructions in the manual, virus production is better than that obtained with many other serum sources. We use the following plasticware for 293FT cells:

T175-Fisher Cat. No. 10-126-13; this is a Falcon flask with 0.2 µm vented plug seal cap.
T75-Fisher Cat. No. 07-200-68; this is a Costar flask with 0.2 µm vented seal cap.
100 mm plate-Fisher Cat. No. 08-772E; this is a Falcon tissue culture-treated polystyrene plate.
We get excellent adherence on these plates under routine cell culture/maintenance conditions.

What are the morphological changes that I should be looking for in my 293FT cells as signs of lentivirus production?

During lentivirus production, 293FT cells undergo the following morphological changes:
- They become multi-nucleated (syncytia development)
- They start to look like balloons or as if they are about to explode
- They often, but not always, lift off from the surface
- Untransfected 293FT cells leave empty spaces and “pile” up at other spots in the flask

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

View all 293FT Cell Line FAQs

引用および参考文献 (3)

引用および参考文献

Abstract

The interaction between HIV-1 Gag and human lysyl-tRNA synthetase during viral assembly.

Authors:Javanbakht H, Halwani R, Cen S, Saadatmand J, Musier-Forsyth K, Gottlinger H, Kleiman L,

Journal:J Biol Chem

PubMed ID:12756246

'Human lysyl-tRNA synthetase (LysRS) is a tRNA-binding protein that is selectively packaged into HIV-1 along with its cognate tRNALys isoacceptors. Evidence exists that Gag alone is sufficient for the incorporation of LysRS into virions. Herein, using both in vitro and in vivo methods, we begin to map regions in Gag ... More

RIP140-targeted repression of gene expression in adipocytes.

Authors:Christian M, Kiskinis E, Debevec D, Leonardsson G, White R, Parker MG,

Journal:Mol Cell Biol

PubMed ID:16227589

'Ligand-dependent repression of nuclear receptor activity forms a novel mechanism for regulating gene expression. To investigate the intrinsic role of the corepressor RIP140, we have monitored gene expression profiles in cells that express or lack the RIP140 gene and that can be induced to undergo adipogenesis in vitro. In contrast ... More

A novel method for long term bone marrow culture and genetic modification of murine neutrophils via retroviral transduction.

Authors:Zemans RL, Briones N, Young SK, Malcolm KC, Refaeli Y, Downey GP, Worthen GS,

Journal:J Immunol Methods

PubMed ID:19010330

'Neutrophils are a critical component of the innate immune response to invading microbial pathogens. However, an excessive and/or prolonged neutrophil response can result in tissue injury that is thought to underlie the pathogenesis of various inflammatory diseases. The development of novel therapeutic strategies for inflammatory diseases depends on an improved ... More