FreeStyle™ 293-F Cells

Citations & References (3)

Gibco™

FreeStyle™ 293-F Cells

FreeStyle™ 293-F細胞株は、293細胞株から派生したもので、FreeStyle™ MAX 293発現システムまたはFreeStyle™ 293発現システムでの使用を目的としています。FreeStyle™ 293-F細胞株は、FreeStyle™詳細を見る

製品番号（カタログ番号）	数量
R79007	1 mL

製品番号（カタログ番号） R79007

価格（JPY）

106,900

お問い合わせください ›

FreeStyle™ 293-F細胞株は、293細胞株から派生したもので、FreeStyle™ MAX 293発現システムまたはFreeStyle™ 293発現システムでの使用を目的としています。FreeStyle™ 293-F細胞株は、FreeStyle™ 293発現培地の懸濁培養に適応しています。凍結細胞は、FreeStyle™ 293発現培地に入れて供給され、直接解凍することができます。FreeStyle™ 293-F細胞株は、1 mLの細胞を含有するバイアルで提供されます。FreeStyle™ 293発現培地90％とDMSO 10％の1 mLあたりに1x10⁷個の生存細胞が存在します。FreeStyle™ 293-F細胞の特性：

• 親の293-F細胞から、限界希釈で再クローン化された低分画のマスターセルバンク培養液から調製しました。。293のクローン由来の培養物は、無血清状態で30～35回の継代のみで維持されます。
•高密度、無血清、懸濁液の増殖に適応し、FreeStyle™ 293発現培地で維持できます。
•FreeStyle™ MAX試薬による高いトランスフェクション効率を実現します。
•懸濁培養物をFreeStyle™ 293発現培地でトランスフェクションでき、培地を交換する必要はありません。。
• 大量の細胞のトランスフェクションを可能にします。

研究用にのみ使用できます。診断用には使用いただけません。

細胞株FreeStyle™ 293-F細胞

使用対象（アプリケーション）タンパク質発現およびトランスフェクション

培地の適応FreeStyle™ 293発現培地

製品ラインFreeStyle

製品タイプ293-F細胞

数量1 mL

システムタイプFreeStyle™

細胞タイプ293-F

Unit SizeEach

組成および保存条件

1x10⁷個の細胞を収めたバイアル1本。液体窒素で保存

よくあるご質問（FAQ）

I do not have FreeStyle 293-F cells. Can I use a different 293 cell line with the FreeStyle 293 Expression System?

Other 293 cell lines may be used with the FreeStyle 293 Expression System. However, they would have to be adapted to serum-free suspension culture in FreeStyle 293 Expression Medium and evaluated for transfection and expression.

How do FreeStyle 293-F cells compare to FreeStyle CHO-S cells in terms of antibody and protein yield?

When comparing FreeStyle 293-F cells with FreeStyle CHO-S cells for average range of antibody and protein yield, it is definitely expected that antibody yield will be higher in FreeStyle 293-F cells than in FreeStyle CHO-S cells, when generated by transient transfection. On the other hand, antibody yields in stable CHO cells can be much higher. For non-antibody proteins, it is impossible to predict which of the two cell lines may provide higher yields.

Can FreeStyle 293-F cells grown in FreeStyle 293 expression medium be transfected with ExpiFectamine293 Reagent along with enhancers? Is so, will the transfection efficiency be higher than that with FreeStyle Max Reagent?

You should be able to use the ExpiFectamine293 Reagent for transfection of Freestyle 293-F cells grown in FreeStyle 293 Expression Medium; however, the enhancers will provide only a little boost in expression as they are designed to work with higher density cultures that FreeStyle 293 Expression Medium cannot support.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

How long can I keep culturing FreeStyle cells after transfection?

We typically keep the culture going for 7-10 days posttransfection. After that, protein expression may drop.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is it necessary to grow FreeStyle 293-F cells in 8% CO2 when using the FreeStyle 293 Expression Medium? If 8% CO2 cannot be arranged, can I supplement the media with an additional reagent?

Our recommendation of 8% CO2 for the optimal growth of Freestyle 293 cells in Freestyle 293 Expression Medium is based on the bicarbonate content in the media and growth conditions of the cells observed by R&D. That said, you may use lower concentrations of CO2 if necessary (5% is common) to grow these cells. You may observe slightly slower growth kinetics or lower cell concentrations, but functionally the entire system will still work.

View all FreeStyle™ 293-F Cells FAQs

引用および参考文献 (3)

引用および参考文献

Abstract

Molecular dissection of the alpha-dystroglycan- and integrin-binding sites within the globular domain of human laminin-10.

Authors:Ido H, Harada K, Futaki S, Hayashi Y, Nishiuchi R, Natsuka Y, Li S, Wada Y, Combs AC, Ervasti JM, Sekiguchi K,

Journal:J Biol Chem

PubMed ID:14701821

'The adhesive interactions of cells with laminins are mediated by integrins and non-integrin-type receptors such as alpha-dystroglycan and syndecans. Laminins bind to these receptors at the C-terminal globular domain of their alpha chains, but the regions recognized by these receptors have not been mapped precisely. In this study, we sought ... More

Biological activity of follistatin isoforms and follistatin-like-3 is dependent on differential cell surface binding and specificity for activin, myostatin, and bone morphogenetic proteins.

Authors:Sidis Y, Mukherjee A, Keutmann H, Delbaere A, Sadatsuki M, Schneyer A,

Journal:Endocrinology

PubMed ID:16627583

Follistatin (FST) and FST-like-3 (FSTL3) are activin-binding and neutralization proteins that also bind myostatin. Three FST isoforms have been described that differ in tissue distribution and cell-surface binding activity, suggesting that the FST isoforms and FSTL3 may have some nonoverlapping biological actions. We produced recombinant FST isoforms and FSTL3 and ... More

Mutation of key residues of RPE65 abolishes its enzymatic role as isomerohydrolase in the visual cycle.

Authors:Redmond TM, Poliakov E, Yu S, Tsai JY, Lu Z, Gentleman S,

Journal:Proc Natl Acad Sci U S A

PubMed ID:16150724

RPE65 is essential for isomerization of vitamin A to the visual chromophore. Mutations in RPE65 cause early-onset blindness, and Rpe65-deficient mice lack 11-cis-retinal but overaccumulate alltrans-retinyl esters in the retinal pigment epithelium (RPE). RPE65 is proposed to be a substrate chaperone but may have an enzymatic role because it is ... More