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Citations & References (17)
Invitrogen™
ProBond™ Nickel-Chelating Resin
ProBond™ Nickel-Chelating Resinはニッケル充填アフィニティーレジンで、ポリヒスチジン（6xHis）配列を有する組換えタンパク質の精製に使用します。レジンに結合したタンパク質は、低pHバッファー、またはイミダゾールやヒスチジンとの競合によって溶出できます。ワンステップの精製は詳細を見る
製品番号（カタログ番号） R80101
価格（JPY）お問い合わせください ›
71,500
Online offer
Ends: 27-Mar-2026
119,200割引額 47,700 (40%)
Each
数量:
50 mL
ProBond™ Nickel-Chelating Resinはニッケル充填アフィニティーレジンで、ポリヒスチジン（6xHis）配列を有する組換えタンパク質の精製に使用します。レジンに結合したタンパク質は、低pHバッファー、またはイミダゾールやヒスチジンとの競合によって溶出できます。ワンステップの精製は、未変性および変性のどちらの条件でも実施できます。ProBond™ Resinは、高クロスリンク6％アガロースレジンに結合したキレートリガンドのイミノ二酢酸（IDA）を使用しており、FPLC、バッチ、および重力流アプリケーションに適しています。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
数量50 mL
固定相ニッケルキレート
形状懸濁
製品ラインProBond
タイプ樹脂
Unit SizeEach
組成および保存条件
ProBond™レジンは事前に帯電されており、レジン1 mlあたり1～5 mgの組換えタンパク質を結合できます。20%エタノール中に50%スラリーとして提供されます。レジンはNi2+で帯電すると、青色に変わります。4℃で保存。ProBond™レジンは、適切に保存した場合に、6ヶ月間の安定が保証されています。
よくあるご質問（FAQ）
How do you typically detect expression of a recombinant fusion protein?
What expression levels can be expected with the pTrcHis/CAT construct?
I purified my protein from a ProBond column using denaturing conditions. After elution, I tried digesting off my N- terminal tag with EKMax Enterokinase, but see no EK cleavage. What can you suggest I try?
Can ProBond or Ni-NTA beads be used for large-scale preparations?
The pH of my ProBond buffers is off. Instead of pH 4-7, it is close to pH 9. What should I do?
引用および参考文献 (17)
引用および参考文献
Abstract
Total synthesis of cyclic ADP-carbocyclic-ribose, a stable mimic of Ca2+-mobilizing second messenger cyclic ADP-ribose.
Journal:J Am Chem Soc
PubMed ID:11535079
'The synthesis of cyclic ADP-carbocyclic-ribose (cADPcR, 4) designed as a stable mimic of cyclic ADP-ribose (cADPR, 1), a Ca2+-mobilizing second messenger, was achieved using as the key step a condensation reaction with the phenylthiophosphate-type substrate 14 to form an intramolecular pyrophosphate linkage. The N-1-carbocyclic-ribosyladenosine derivative 16 was prepared via the
Thr-161 Phosphorylation of Monomeric Cdc2. REGULATION BY PROTEIN PHOSPHATASE 2C IN XENOPUS OOCYTES.
Journal:J Biol Chem
PubMed ID:12036957
'Fully grown Xenopus oocyte is arrested at prophase I of meiosis. Re-entry into meiosis depends on the activation of MPF (M-phase promoting factor or cyclin B.Cdc2 complex), triggered by progesterone. The prophase-arrested oocyte contains a store of Cdc2. Most of the protein is present as a monomer whereas a minor
Neutrophil phospholipase D is activated by a membrane-associated Rho family small molecular weight GTP-binding protein.
Journal:J Biol Chem
PubMed ID:8408000
'Phospholipase D in human neutrophil lysates is activated by GTP gamma S (guanosine 5''-O-(3-thiotriphosphate)), implying the participation of a GTP-binding protein. Reconstitution of GTP gamma S-dependent activity requires protein factors in both the plasma membrane and the cytosol (Olson, S. C., Bowman, E. P., and Lambeth, J. D. (1991) J.
The plasmid RK2 initiation protein binds to the origin of replication as a monomer.
Journal:J Biol Chem
PubMed ID:8636140
'The TrfA protein encoded by the broad host range bacterial plasmid RK2 specifically binds to eight direct repeats (iterons) present at the plasmid replication origin to initiate DNA replication. Purified TrfA protein is largely in the form of a dimer, and using a dimerization test system that involves the fusion
Saccharomyces cerevisiae cytoplasmic tyrosyl-tRNA synthetase gene. Isolation by complementation of a mutant Escherichia coli suppressor tRNA defective in aminoacylation and sequence analysis.
Journal:J Biol Chem
PubMed ID:8509419
'Exploiting differences in tRNA recognition between prokaryotic and eukaryotic tyrosyl-tRNA synthetases (TyrRSs), we have isolated the gene for the cytoplasmic TyrRS of Saccharomyces cerevisiae by functional complementation in Escherichia coli of a mutant E. coli tRNA. The tRNA, derived from the E. coli initiator tRNA with changes to allow suppression