ProBond™ Nickel-Chelating Resin

Citations & References (5)

Invitrogen™

ProBond™ Nickel-Chelating Resin

Name: ProBond™ Nickel-Chelating Resin
SKU: R80115

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製品番号（カタログ番号）	数量
R80115	150 mL
R80101	50 mL

2 オプション

製品番号（カタログ番号） R80115

価格（JPY）

302,500

Each

お問い合わせください ›

数量:

150 mL

一括またはカスタム形式をリクエストする

ProBond™ Nickel-Chelating Resinはニッケル充填アフィニティーレジンで、ポリヒスチジン（6xHis）配列を有する組換えタンパク質の精製に使用します。レジンに結合したタンパク質は、低pHバッファー、またはイミダゾールやヒスチジンとの競合によって溶出できます。ワンステップの精製は、未変性および変性のどちらの条件でも実施できます。ProBond™ Resinは、高クロスリンク6％アガロースレジンに結合したキレートリガンドのイミノ二酢酸（IDA）を使用しており、FPLC、バッチ、および重力流アプリケーションに適しています。

研究用にのみ使用できます。診断用には使用いただけません。

仕様

数量150 mL

固定相ニッケルキレート

カラムタイプアフィニティカラム

形状懸濁

製品ラインProBond

タイプProBond Nickel-Chelating Resin

Unit SizeEach

組成および保存条件

ProBond™レジンは事前に帯電されており、レジン1 mlあたり1～5 mgの組換えタンパク質を結合できます。20%エタノール中に50%スラリーとして提供されます。レジンはNi²⁺で帯電すると、青色に変わります。4℃で保存。ProBond™レジンは、適切に保存した場合に、6ヶ月間の安定が保証されています。

よくあるご質問（FAQ）

How do you typically detect expression of a recombinant fusion protein?

Western blot analysis is typically used to detect the expressed protein. We sell several antibodies against various epitopes, such as Xpress, HisG, V5, or C-terminal 6xHis. Additionally, His-tagged proteins can be purified using our ProBond Purification System via affinity purification.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What expression levels can be expected with the pTrcHis/CAT construct?

In one experiment, 35 µg CAT/mg total protein or 68.2 mg CAT/liter of culture was obtained. 50 ml of cell extract was loaded onto a ProBond column and 2 mg of CAT was recovered. The eluted protein appeared reasonably pure on a gel.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

I purified my protein from a ProBond column using denaturing conditions. After elution, I tried digesting off my N- terminal tag with EKMax Enterokinase, but see no EK cleavage. What can you suggest I try?

The enzyme could be denatured. Try buffer exchange or dialysis before digestion with EKMax Enterokinase.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can ProBond or Ni-NTA beads be used for large-scale preparations?

ProBond and Ni-NTA beads can be used in FPLC columns. However, the beads can only withstand low pressure (~43.5 psi max).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

The pH of my ProBond buffers is off. Instead of pH 4-7, it is close to pH 9. What should I do?

pH drift is typical with these buffers. Adjust with concentrated HCl if the pH is too high or with 10 N NaOH if the pH is low.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all ProBond™ Nickel-Chelating Resin, 150 mL FAQs

引用および参考文献 (5)

引用および参考文献

Abstract

Total synthesis of cyclic ADP-carbocyclic-ribose, a stable mimic of Ca2+-mobilizing second messenger cyclic ADP-ribose.

Authors: Shuto S; Fukuoka M; Manikowsky A; Ueno Y; Nakano T; Kuroda R; Kuroda H; Matsuda A;

Journal:J Am Chem Soc

PubMed ID:11535079

'The synthesis of cyclic ADP-carbocyclic-ribose (cADPcR, 4) designed as a stable mimic of cyclic ADP-ribose (cADPR, 1), a Ca2+-mobilizing second messenger, was achieved using as the key step a condensation reaction with the phenylthiophosphate-type substrate 14 to form an intramolecular pyrophosphate linkage. The N-1-carbocyclic-ribosyladenosine derivative 16 was prepared via the ... More

Interaction between the insulin receptor and its downstream effectors. Use of individually expressed receptor domains for structure/function analysis.

Authors:Paz K, Voliovitch H, Hadari YR, Roberts CT Jr, LeRoith D, Zick Y

Journal:J Biol Chem

PubMed ID:8636129

'A structural analysis has been carried out to determine which part of the intracellular domain of the insulin receptor (IR) beta subunit is involved in direct interaction with the receptor substrates IRS-1 and Shc. Toward this end, the juxtamembrane (JM) domain (amino acids 943- 984) and the carboxyl-terminal (CT) region ... More

A Calcium-Responsive Transcription Factor, CaRF, that Regulates Neuronal Activity-Dependent Expression of BDNF.

Authors: Tao Xu; West Anne E; Chen Wen G; Corfas Gabriel; Greenberg Michael E;

Journal:Neuron

PubMed ID:11832226

'Transcription of the brain-derived neurotrophic factor (BDNF) gene is regulated in a calcium- and neuron-selective manner; however, the mechanisms that underlie this selectivity are not known. We have characterized a new calcium-response element, CaRE1, that is required for activity-dependent transcription of BDNF exon III and have cloned a transcription factor, ... More

Tryptophan fluorescence reports nucleotide-induced conformational changes in a domain of the ArsA ATPase.

Authors:Zhou T, Rosen BP

Journal:J Biol Chem

PubMed ID:9242630

The ars operon of plasmid R773 encodes an ATP-dependent extrusion pump for arsenite and antimonite in Escherichia coli. The ArsA ATPase is the catalytic subunit of the pump protein, with two nucleotide binding consensus sequences, one in the NH2-terminal half and one in the COOH- terminal half of the protein. ... More

Structure and ligand of a histone acetyltransferase bromodomain.

Authors:Dhalluin C, Carlson JE, Zeng L, He C, Aggarwal AK, Zhou MM

Journal:Nature

PubMed ID:10365964

Histone acetylation is important in chromatin remodelling and gene activation. Nearly all known histone-acetyltransferase (HAT)-associated transcriptional co-activators contain bromodomains, which are approximately 110-amino-acid modules found in many chromatin-associated proteins. Despite the wide occurrence of these bromodomains, their three-dimensional structure and binding partners remain unknown. Here we report the solution structure ... More