Ni-NTA Agarose

Citations & References (4)

Invitrogen™

Ni-NTA Agarose

Name: Ni-NTA Agarose
SKU: R90101

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製品番号（カタログ番号）	数量
R90101	10 mL
R90115	25 mL
R90110	100 mL

3 オプション

製品番号（カタログ番号） R90101

価格（JPY）

22,700

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Ends: 27-Mar-2026

37,900

割引額 15,200 (40%)

Each

お問い合わせください ›

数量:

10 mL

一括またはカスタム形式をリクエストする

Ni-NTAアガロースは、ニッケルでチャージされたアフィニティーレジンで、ポリヒスチジン（6xHis）配列を含む組換えタンパク質の精製に使用できます。レジンに結合したタンパク質は、低pHバッファー、またはイミダゾールやヒスチジンとの競合によって溶出できます。ワンステップの精製は、未変性および変性のどちらの条件でも実施できます。Ni-NTAは、クロスリンク型6%アガロースレジンに結合した、キレートリガンドのニトロトリ酢酸（NTA）を使用し、バッチおよび重力落下アプリケーションに適しています。

研究用にのみ使用できます。診断用には使用いただけません。

仕様

数量10 mL

固定相Ni-NTA

カラムタイプAffinity Column

形状懸濁

タイプアガロース

Unit SizeEach

組成および保存条件

Ni-NTAレジンは帯電済みで、レジン1 mLあたり最大50 mgの組換えタンパク質を結合できます。30%エタノール中に50%スラリーとして提供されます。レジンはNi²⁺で帯電すると、青色に変わります。4℃で保存。Ni-NTAレジンは、適切に保存した場合、6ヶ月間の安定性が保証されています。

よくあるご質問（FAQ）

Are there other sequences that can bind to nickel more tightly than 6xHis-tagged proteins and how can they be eluted.

Yes, 7xHis-tagged proteins, proteins naturally high in histidine, and other combinations of His and other amino acids will bind. To elute them, you have to increase the concentration of imidazole. Generally these peptides will not contaminate your fraction since they remain on the column. However after multiple uses of the same column, these peptides may reduce the binding capacity of the column.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can ProBond or Ni-NTA beads be used for large-scale preparations?

ProBond and Ni-NTA beads can be used in FPLC columns. However, the beads can only withstand low pressure (~43.5 psi max).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What should the typical protein recovery be when using the Probond Purification System or Ni-NTA Purification system?

Both systems are qualified by purifying 2 mg of myoglobin protein on a column and performing a Bradford assay. Protein recovery must be 75% or higher.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

引用および参考文献 (4)

引用および参考文献

Abstract

A protein kinase encoded by the t complex responder gene causes non-mendelian inheritance

Authors:BERNHARD G. HERRMANN, BIRGIT KOSCHORZ, KARIN WERTZ, K. JOHN MCLAUGHLIN* & ANDREAS KISPERT

Journal:Nature

PubMed ID:10647005

'Males heterozygous for the t-haplotype form of mouse chromosome 17 preferentially transmit the t-chromosome to their progeny. Several distorter/sterility loci carried on the t-haplotype together impair flagellar function in all spermatozoa whereas the responder, Tcr, rescues t-sperm but not wild-type sperm. Thus, t-sperm have anadvantage over wild-type sperm in fertilizing ... More

DNA Polymerase lambda, a Novel DNA Repair Enzyme in Human Cells.

Authors: Garcia-Diaz Miguel; Bebenek Katarzyna; Sabariegos Rosario; Dominguez Orlando; Rodriguez Josana; Kirchhoff Tomas; Garcia-Palomero Esther; Picher Angel J; Juarez Raquel; Ruiz Jose F; Kunkel Thomas A; Blanco Luis;

Journal:J Biol Chem

PubMed ID:11821417

'DNA polymerase lambda (pol lambda) is a novel family X DNA polymerase that has been suggested to play a role in meiotic recombination and DNA repair. The recent demonstration of an intrinsic 5''-deoxyribose-5-phosphate lyase activity in pol lambda supports a function of this enzyme in base excision repair. However, the ... More

A ribozyme that ligates RNA to protein.

Authors: Baskerville Scott; Bartel David P;

Journal:Proc Natl Acad Sci U S A

PubMed ID:12077317

'We have used a combination of in vitro selection and rational design to generate ribozymes that form a stable phosphoamide bond between the 5'' terminus of an RNA and a specific polypeptide. This reaction differs from that of previously identified ribozymes, although the product is analogous to the enzyme-nucleotidyl intermediates ... More

Transduction of MIN6 beta Cells with TAT-Syntaxin SNARE Motif Inhibits Insulin Exocytosis in Biphasic Insulin Release in a Distinct Mechanism Analyzed by Evanescent Wave Microscopy.

Authors: Ohara-Imaizumi Mica; Nakamichi Yoko; Nishiwaki Chiyono; Nagamatsu Shinya;

Journal:J Biol Chem

PubMed ID:12393909

To investigate the in vivo interaction of syntaxin-mediated soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) assembly and insulin exocytosis in biphasic release, we examined the dynamics of insulin granule motion such as docking and fusion with the plasma membrane when the syntaxin SNARE motif (H3 domain) was transduced into ... More