Streptavidin, Pacific Blue™ conjugate

Citations & References (8)

Streptavidin, Pacific Blue™ conjugate

当社のストレプトアビジン、Pacific Blue™コンジュゲートは、励起／発光約410/455 nmの明るい青色蛍光プローブです。ストレプトアビジンは、細菌から単離され、中性に近い等電点を持つ非グリコシル化ビオチン結合タンパク質です。グリコシル化されたビオチン結合タンパク質であるアビジンよりも非特異的結合が少ないと報告されているため、ビオチン化プローブの検出に広く使用されています詳細を見る

製品番号（カタログ番号）	数量
S11222	1 mg

製品番号（カタログ番号） S11222

価格（JPY）

67,000

お問い合わせください ›

当社のストレプトアビジン、Pacific Blue™コンジュゲートは、励起／発光約410/455 nmの明るい青色蛍光プローブです。ストレプトアビジンは、細菌から単離され、中性に近い等電点を持つ非グリコシル化ビオチン結合タンパク質です。グリコシル化されたビオチン結合タンパク質であるアビジンよりも非特異的結合が少ないと報告されているため、ビオチン化プローブの検出に広く使用されています。

すべてのAlexa Fluor™ストレプトアビジンおよびストレプトアビジンコンジュゲートの選択ガイドをご覧ください。

研究用途にのみご使用ください。診断目的には使用できません。

概要Pacific Blue™コンジュゲートストレプトアビジン、1 mg、イエロー、固体、407nm吸収光、Pacific Blue™標識または色素、励起／発光410/455nm、ブロット分析、フローサイトメトリー、組織化学アプリケーション、イムノアッセイに使用、保存温度は–5～–30℃

検出法蛍光

励起／発光410/455 nm

フローサイトメーターレーザーライン405

使用対象（アプリケーション）フローサイトメトリー、免疫測定法、組織化学アプリケーション、ブロット分析

標識または色素Pacific Blue

パッケージ各1

製品タイプストレプトアビジンコンジュゲート

純度または品質グレードTLC

数量1 mg

規制状況研究用途にのみご使用ください（RUO）

サンプル量1 mg

品質保持期間6カ月

出荷条件室温

保存バッファー10mg/mL PBS

コンジュゲートPacific Blue

製品ラインPacific Blue

Unit SizeEach

組成および保存条件

ストレプトアビジンPacific Blue™コンジュゲート1バイアルを含みます。

フリーザー（-5～-30度）に保存し、遮光してください。

よくあるご質問（FAQ）

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

引用および参考文献 (8)

引用および参考文献

Abstract

Arenavirus nucleoprotein targets interferon regulatory factor-activating kinase IKKe.

Authors:Pythoud C, Rodrigo WW, Pasqual G, Rothenberger S, Martínez-Sobrido L, de la Torre JC, Kunz S,

Journal:J Virol

PubMed ID:22532683

'Arenaviruses perturb innate antiviral defense by blocking induction of type I interferon (IFN) production. Accordingly, the arenavirus nucleoprotein (NP) was shown to block activation and nuclear translocation of interferon regulatory factor 3 (IRF3) in response to virus infection. Here, we sought to identify cellular factors involved in innate antiviral signaling ... More

In vivo expansion of T reg cells with IL-2-mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression.

Authors:Webster KE, Walters S, Kohler RE, Mrkvan T, Boyman O, Surh CD, Grey ST, Sprent J,

Journal:J Exp Med

PubMed ID:19332874

'Via a transcription factor, Foxp3, immunoregulatory CD4(+)CD25(+) T cells (T reg cells) play an important role in suppressing the function of other T cells. Adoptively transferring high numbers of T reg cells can reduce the intensity of the immune response, thereby providing an attractive prospect for inducing tolerance. Extending our ... More

Rapid lytic granule convergence to the MTOC in natural killer cells is dependent on dynein but not cytolytic commitment.

Authors:Mentlik AN, Sanborn KB, Holzbaur EL, Orange JS,

Journal:Mol Biol Cell

PubMed ID:20444980

'Natural killer cells are lymphocytes specialized to participate in host defense through their innate ability to mediate cytotoxicity by secreting the contents of preformed secretory lysosomes (lytic granules) directly onto a target cell. This form of directed secretion requires the formation of an immunological synapse and occurs stepwise with actin ... More

Immunosuppressive property within the Streptococcus pneumoniae cell wall that inhibits generation of T follicular helper, germinal center, and plasma cell response to a coimmunized heterologous protein.

Authors:Saumyaa, Arjunaraja S, Pujanauski L, Colino J, Torres RM, Snapper CM,

Journal:

PubMed ID:23817619

'We previously demonstrated that intact, inactivated Streptococcus pneumoniae (unencapsulated strain R36A) inhibits IgG responses to a number of coimmunized soluble antigens (Ags). In this study, we investigated the mechanism of this inhibition and whether other extracellular bacteria exhibited similar effects. No inhibition was observed if R36A was given 24 h ... More

Thymocytes may persist and differentiate without any input from bone marrow progenitors.

Authors:Peaudecerf L, Lemos S, Galgano A, Krenn G, Vasseur F, Di Santo JP, Ezine S, Rocha B,

Journal:J Exp Med

PubMed ID:22778388

'Thymus transplants can correct deficiencies of the thymus epithelium caused by the complete DiGeorge syndrome or FOXN1 mutations. However, thymus transplants were never used to correct T cell-intrinsic deficiencies because it is generally believed that thymocytes have short intrinsic lifespans. This notion is based on thymus transplantation experiments where it ... More

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