SYTO™ 82 Orange Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO
SYTO™ 82 Orange Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO
Citations & References (22)
Invitrogen™

SYTO™ 82 Orange Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

細胞透過性SYTO82オレンジ蛍光核酸染色剤は、核酸に結合すると明るいオレンジ色の蛍光を示します。生細胞におけるSYTO色素の染色パターンは細胞タイプによって異なる可能性があるため、当社ではSYTOオレンジ色の蛍光核酸染色サンプラーキット（S-11360）を提供しており、研究者はシステムに最適なオレンジ蛍光SYTO染色を見つけることができます。詳細を見る
テクニカルサポートオーダーサポート
製品番号（カタログ番号）数量
S11363250 μL
製品番号（カタログ番号） S11363
価格（JPY）
74,100
Each
お問い合わせください ›
数量:
250 μL
細胞透過性SYTO82オレンジ蛍光核酸染色剤は、核酸に結合すると明るいオレンジ色の蛍光を示します。生細胞におけるSYTO色素の染色パターンは細胞タイプによって異なる可能性があるため、当社ではSYTOオレンジ色の蛍光核酸染色サンプラーキット（S-11360）を提供しており、研究者はシステムに最適なオレンジ蛍光SYTO染色を見つけることができます。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
色オレンジ
概要SYTO™ 82 Orange Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO
検出法蛍光
染色剤タイプ細胞透過性
発光560 nm
励起波長域541⁄560
使用対象 (装置)蛍光顕微鏡
製品ラインSYTO
数量250 μL
出荷条件室温
容量（メートル法）250 μL
標識タイプFluorescent Dye
製品タイプ核酸染色
SubCellular Localization核酸
Unit SizeEach
組成および保存条件
フリーザー(-5°C∼-30°C)に保存し、遮光してください。

よくあるご質問（FAQ）

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use SYTO 82 dye for flow cytometry to measure DNA content?

SYTO 82 dye tends to stain RNA as well as DNA. Better options for cell permeant nucleic acid stains for flow cytometry analysis of DNA content are the cell cycle stains such as:
- Vybrant DyeCycle Violet Stain (Cat. No. V35003)
- Vybrant DyeCycle Green Stain (Cat. No. V35004)
- Vybrant DyeCycle Orange Stain (Cat. No. V35005)
- Vybrant DyeCycle Ruby Stain (Cat. No. V10273)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (22)

引用および参考文献
Abstract
Comparison of multiple DNA dyes for real-time PCR: effects of dye concentration and sequence composition on DNA amplification and melting temperature.
Authors:Gudnason H,Dufva M,Bang DD,Wolff A
Journal:Nucleic acids research
PubMed ID:17897966
The importance of real-time polymerase chain reaction (PCR) has increased steadily in clinical applications over the last decade. Many applications utilize SYBR Green I dye to follow the accumulation of amplicons in real time. SYBR Green I has, however, a number of limitations that include the inhibition of PCR, preferential ... More
Imaging Poliovirus Entry in Live Cells.
Authors:Brandenburg B, Lee LY, Lakadamyali M, Rust MJ, Zhuang X, Hogle JM
Journal:PLoS Biol
PubMed ID:17622193
'Viruses initiate infection by transferring their genetic material across a cellular membrane and into the appropriate compartment of the cell. The mechanisms by which animal viruses, especially nonenveloped viruses, deliver their genomes are only poorly understood. This is due in part to technical difficulties involved in direct visualization of viral ... More
Mitotic spindle rotation and mode of cell division in the developing telencephalon.
Authors:Haydar TF, Ang E, Rakic P
Journal:Proc Natl Acad Sci U S A
PubMed ID:12589023
'The mode of neural stem cell division in the forebrain proliferative zones profoundly influences neocortical growth by regulating the number and diversity of neurons and glia. Long-term time-lapse multiphoton microscopy of embryonic mouse cortex reveals new details of the complex three-dimensional rotation and oscillation of the mitotic spindle before stem ... More
A loop-mediated isothermal amplification (LAMP) assay for Strongyloides stercoralis in stool that uses a visual detection method with SYTO-82 fluorescent dye.
Authors:Watts MR, James G, Sultana Y, Ginn AN, Outhred AC, Kong F, Verweij JJ, Iredell JR, Chen SC, Lee R,
Journal:
PubMed ID:24323513
'An assay to detect Strongyloides stercoralis in stool specimens was developed using the loop-mediated isothermal amplification (LAMP) method. Primers were based on the 28S ribosomal subunit gene. The reaction conditions were optimized and SYTO-82 fluorescent dye was used to allow real-time and visual detection of the product. The product identity ... More
High-resolution melting curve analysis, a rapid and affordable method for mutation analysis in childhood acute myeloid leukemia.
Authors:Liu Y, Tang J, Wakamatsu P, Xue H, Chen J, Gaynon PS, Shen S, Sun W,
Journal:
PubMed ID:25250304
'Molecular genetic alterations with prognostic significance have been described in childhood acute myeloid leukemia (AML). The aim of this study was to establish cost-effective techniques to detect mutations of FMS-like tyrosine kinase 3 (FLT3), nucleophosmin 1 (NPM1), and a partial tandem duplication within the mixed-lineage leukemia (MLL-PTD) genes in childhood ... More
View all SYTO™ 82 Orange Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO citations