SYBR™ Gold Nucleic Acid Gel Stain (10,000X Concentrate in DMSO)

Citations & References (60)

Invitrogen™

SYBR™ Gold Nucleic Acid Gel Stain (10,000X Concentrate in DMSO)

SYBR™ ゴールド核酸ゲル染色剤は、核酸の検出に最も高感度な蛍光染色剤です。UVトランスイルミネーター、ダークリーダー（下記の染色核酸ゲルを参照）、レーザースキャナー、または Safe詳細を見る

製品番号（カタログ番号）	数量
S11494	500 μL

製品番号（カタログ番号） S11494

価格（JPY）

45,100

お問い合わせください ›

500 μL

一括またはカスタム形式をリクエストする

SYBR™ ゴールド核酸ゲル染色剤は、核酸の検出に最も高感度な蛍光染色剤です。UVトランスイルミネーター、ダークリーダー（下記の染色核酸ゲルを参照）、レーザースキャナー、または Safe Imager 2.0™や E-Gel Imager™などの青色光トランスイルミネーターで使用します。

SYBR™ ゴールド核酸ゲル染色剤の特長：

• 超高感度：エチジウムブロマイドの 25–100倍の感度。わずか25 pgのDNAを検出
• DNAクローニング効率の向上：DNA損傷の原因とならない青色光透過性
• シグナル／バックグラウンド比の増加：核酸に結合した場合の1,000倍を超えるシグナル増強
• 汎用性：グリオキサールゲル、ホルムアルデヒドゲル、尿素ゲル中の本来のdsDNA、ssDNA、RNAを検出します

染色特性
SYBR™ Gold染色剤は、独自の非対称シアニン色素で、核酸結合時に1,000倍を超える蛍光増強を示し、二本鎖または一本鎖DNAまたはRNA¹に結合すると、高い量子収率（約0.6）を示します。色素 - 核酸複合体の励起極大は ∼495 nmおよび ∼300 nmで、最大蛍光波長は ∼537 nmです（スペクトルを参照）。

染色感度
SYBR™ Gold 染色試薬は、300 nmのトランスイルミネーションを使用した場合でも、尿素、グリオキサール、ホルムアルデヒドの変性ゲル中のDNAおよびRNAを検出するためのエチジウムブロマイドよりも >10倍高い感度を示します（下記のグリオキサル酸RNAの可視化を参照）。SYBR™ Gold 染色試薬™ は、一本鎖DNA高次構造多型（SSCP）製品を検出するための SYBR Green II 染色よりもはるかに高感度であることが示されています（下図参照）。さらに、天然のポリアクリルアミドゲルで二本鎖DNAを検出する場合は、シルバー染色よりもはるかに高い感度を示します（感度の比較を参照）。

¹Tuma RS、Beaudet MP、Jin X、Jones LJ、Cheung Cy、Yue S、Singer VL 。Characterization of SYBR Gold nucleic acid gel stain: a dye optimized for use with 300-nm ultraviolet transilluminators.Anal Biochem (1999) 268:278-288.(PMID: 10075818)

研究用にのみ使用できます。診断用には使用いただけません。

検出位置ゲル内検出

検出法蛍光

製品タイプ核酸ゲル染色

数量500 μL

出荷条件室温

標的分子RNA、DNA

標識または色素SYBRゴールド

Unit SizeEach

組成および保存条件

DMSOで10,000倍濃縮液として提供されます。乾燥器内の遮光された-20℃で保存します。

よくあるご質問（FAQ）

What is the pH range of SYBR dyes?

The SYBR dyes are useful only over a narrow range of pH, from about 7 to 8. Outside this range, the fluorescent signal diminishes rapidly.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the recommended filter for my gel documentation system?

Please go here (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-gel-electrophoresis/dna-stains/sybr-safe.html) and click on the “Filter Recommendations” tab to see filter recommendations for use with SYBR Safe DNA Gel Stain. Note that the excitation and emission spectra of SYBR Safe DNA Gel Stain are very similar to those of SYBR Green I, SYBR Green II, and SYBR Gold dyes, as well as fluorescein (FITC). Therefore, filters appropriate for these dyes can also be used. A camera filter is not required with the Safe Imager Blue-Light Transilluminator; the amber filter provided with the instrument serves this purpose.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Can I use the ethidium bromide filters on my camera to image SYBR dyes?

This is not recommended. Most deep amber/orange ethidium bromide filters have a cutoff value around 550 nm. The SYBR Green dyes emit at 520 nm, which will not be detected using this filter.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Is SYBR Safe DNA Gel Stain the same as SYBR Green I dye?

All SYBR dyes have similar spectral properties, but have different chemical compositions. All SYBR dyes bind to dsDNA, ssDNA and RNA but vary in sensitivity. SYBR Safe DNA Gel Stain (Cat. No. S33102) was specifically developed as a safer alternative to ethidium bromide. SYBR Green I (Cat. No. S7585) is an ultrasensitive stain for dsDNA, and SYBR Green II (Cat. No. S7564) is a highly sensitive stain for RNA and ssDNA. All SYBR dyes are optimally excited by the Safe Imager Blue-Light Transilluminator.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

How should I dispose of SYBR DNA Gel Stain?

Disposal regulations vary. Please contact your safety office or local municipality for disposal guidelines.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

View all SYBR™ Gold Nucleic Acid Gel Stain (10,000X Concentrate in DMSO) FAQs

引用および参考文献 (60)

引用および参考文献

Abstract

Authors:

Journal:

PubMed ID:16517648

The transcriptional coactivator CREB-binding protein cooperates with STAT1 and NF-kappa B for synergistic transcriptional activation of the CXC ligand 9/monokine induced by interferon-gamma gene.

Authors:Hiroi M, Ohmori Y

Journal:J Biol Chem

PubMed ID:12403783

'Signal transducers and activators of transcription 1 (STAT1) and NF-kappaB cooperatively regulate the expression of many inflammatory genes. In the present study, we demonstrate that the transcriptional coactivator CREB-binding protein (CBP) mediated the STAT1/NF-kappaB synergy for transcription of the gene for CXC ligand 9 (CXCL9), an interferon-gamma (IFN-gamma)-inducible chemokine. Reporter ... More

Gel staining methods for detection of telomerase activity with the telomeric repeat amplification protocol (TRAP) assay.

Authors:Fujita M, Tomita S, Ueda Y, Fujimori T

Journal:Mol Pathol

PubMed ID:10193516

DOTAP cationic liposomes prefer relaxed over supercoiled plasmids.

Authors:Even-Chen S, Barenholz Y

Journal:Biochim Biophys Acta

PubMed ID:11118529

'Cationic liposomes and DNA interact electrostatically to form complexes called lipoplexes. The amounts of unbound (free) DNA in a mixture of cationic liposomes and DNA at different cationic lipid:DNA molar ratios can be used to describe DNA binding isotherms; these provide a measure of the binding efficiency of DNA to ... More

Application of digital image analysis and flow cytometry to enumerate marine viruses stained with SYBR gold.

Authors:Chen F, Lu JR, Binder BJ, Liu YC, Hodson RE

Journal:Appl Environ Microbiol

PubMed ID:11157214

'A novel nucleic acid stain, SYBR Gold, was used to stain marine viral particles in various types of samples. Viral particles stained with SYBR Gold yielded bright and stable fluorescent signals that could be detected by a cooled charge-coupled device camera or by flow cytometry. The fluorescent signal strength of ... More

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