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Citations & References (163)
Invitrogen™
Succinimidyl 3-(2-Pyridyldithio)Propionate (SPDP)
ヘテロ二官能性の架橋剤スクシンイミジル3-（2-ピリジルジチオ）プロピオン酸（SPDP）により、1つを1つのターゲットグループに共有結合できます。スクシンイミジルエステルは、一級アミンに化学的に反応し、ジチオールを穏やかに還元すると、他のチオールでジスルフィドを形成できます詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| S1531 | 100 mg |
製品番号（カタログ番号） S1531
価格（JPY）お問い合わせください ›
61,000
Each
数量:
100 mg
ヘテロ二官能性の架橋剤スクシンイミジル3-（2-ピリジルジチオ）プロピオン酸（SPDP）により、1つを1つのターゲットグループに共有結合できます。スクシンイミジルエステルは、一級アミンに化学的に反応し、ジチオールを穏やかに還元すると、他のチオールでジスルフィドを形成できます。架橋試薬は、生体高分子内および生体高分子間の空間的な関係や相互作用をのプロービングに役立ちます。
研究用途にのみご使用ください。診断目的には使用できません。
仕様
細胞透過性Yes
概要SPDP
形状Solid
標識法Chemical Labeling
分子量312.36
PEG化No
数量100 mg
反応性部分NHS Ester, Pyridyldisulfide
溶解性DMF、DMSO
スペーサーアーム長6.8 Å
水溶性No
化学反応性Amine-Sulfhydryl
CleavableBy Thiols
クロスリンカータイプヘテロ三官能性
フォーマットStandard
製品タイプ架橋剤
スペーサーShort (<10 Å)
Unit SizeEach
組成および保存条件
Upon receipt store at -20°C protected from light.
よくあるご質問（FAQ）
Can you provide the shelf-life for Succinimidyl 3-(2-Pyridyldithio)Propionate (SPDP)?
引用および参考文献 (163)
引用および参考文献
Abstract
Blockade of the galactose-binding sites of ricin by its linkage to antibody. Specific cytotoxic effects of the conjugates.
Journal:European journal of biochemistry
PubMed ID:6608447
A method is described for preparing specific cytotoxic agents by linking intact ricin to antibodies in a manner that produces obstruction of the galactose-binding sites on the B chain of the toxin and so diminishes the capacity of the conjugate to bind non-specifically to cells. The conjugates were synthesised by
Enhancement of the thrombolytic potency of plasminogen activators by conjugation with clot-specific monoclonal antibodies.
Journal:Bioconjug Chem
PubMed ID:1790167
Detection of one attomole of [Arg8]-vasopressin by novel noncompetitive enzyme immunoassay (hetero-two-site complex transfer enzyme immunoassay).
Journal:J Biochem (Tokyo)
PubMed ID:1778973
'One attomole of [Arg8]-vasopressin (AVP) was detected by a novel noncompetitive enzyme immunoassay (hetero-two-site complex transfer enzyme immunoassay). AVP was indirectly biotinylated using N-hydroxysuccinimidobiotin and trapped onto an anti-AVP IgG-coated polystyrene ball. After washing, biotinylated AVP was eluted from the polystyrene ball with HCl and was reacted with 2,4-dinitrophenyl-fluorescein disulfide-bovine
Activation of methotrexate-alpha-alanine by carboxypeptidase A-monoclonal antibody conjugate.
Journal:Biochemistry
PubMed ID:1731945
'Carboxypeptidase A (CP-A) and monoclonal antibody KS1/4 directed against an antigen on human lung adenocarcinoma cells (UCLA-P3) were derivatized by treatment with succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate and N-succinimidyl 3-(2-pyridyldithio)propionate, respectively. The derivatized proteins were reacted to produce thioether-linked enzyme-antibody conjugates. Sequential HPLC size-exclusion and DEAE chromatography separated the conjugate preparation from unreacted
Selective destruction of nerve growth factor receptor-bearing cells in vitro using a hybrid toxin composed of ricin A chain and a monoclonal antibody against the nerve growth factor receptor.
Journal:J Cell Biol
PubMed ID:2993314
'A hybrid toxin composed of ricin A chain and a monoclonal antibody directed against the rat nerve growth factor (NGF) receptor (192-IgG) was prepared using the heterobifunctional cross-linking agent N-succinimidyl-3-(2-pyridyldithio)-propionate and purified by affinity chromatography. Characterization studies showed that the hybrid, 192-s-s-A, displaced bound 125I-labeled 192-IgG from rat superior cervical