I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?
In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.
I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?
The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).
引用および参考文献 (23)
引用および参考文献
Abstract
Printing protein arrays from DNA arrays.
Authors:He M, Stoevesandt O, Palmer EA, Khan F, Ericsson O, Taussig MJ,
Journal:Nat Methods
PubMed ID:18204456
'We describe a method, DNA array to protein array (DAPA), which allows the ''printing'' of replicate protein arrays directly from a DNA array template using cell-free protein synthesis. At least 20 copies of a protein array can be obtained from a single DNA array. DAPA eliminates the need for separate ... More
Comparison of hydroxylated print additives on antibody microarray performance.
Authors:Wu P, Grainger DW,
Journal:J Proteome Res
PubMed ID:17081047
'Various hydroxylated additives were added to antibody print buffers at different concentrations to stabilize printed antibodies during normal array spot desiccation on commercial polymer-coated microarray slides. Polyvinyl alcohol addition to print buffers produced the most regular spot morphologies, homogeneous intra-spot antibody distribution, uniform fluorescence intensity, and improved analyte capture activity, ... More
The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface.
Authors:Unternaehrer JJ, Chow A, Pypaert M, Inaba K, Mellman I
Journal:Proc Natl Acad Sci U S A
PubMed ID:17190803
'We have found that MHC class II (MHC II) molecules exhibit a distinctive organization on the dendritic cell (DC) plasma membrane. Both in DC lysates and on the surface of living cells, I-A and I-E molecules engaged in lateral interactions not observed on other antigen-presenting cells such as B blasts. ... More
Development of homogeneous binding assays based on fluorescence resonance energy transfer between quantum dots and Alexa Fluor fluorophores.
Authors:Nikiforov TT, Beechem JM
Journal:Anal Biochem
PubMed ID:16860286
'We studied the fluorescence resonance energy transfer (FRET) between quantum dots emitting at 565, 605, and 655 nm as energy donors and Alexa Fluor fluorophores with absorbance maxima at 594, 633, 647, and 680 nm as energy acceptors. As a first step, we prepared covalent conjugates between all three types ... More
Restriction of receptor movement alters cellular response: physical force sensing by EphA2.
Authors:Salaita K, Nair PM, Petit RS, Neve RM, Das D, Gray JW, Groves JT,
Journal:Science
PubMed ID:20223987
'Activation of the EphA2 receptor tyrosine kinase by ephrin-A1 ligands presented on apposed cell surfaces plays important roles in development and exhibits poorly understood functional alterations in cancer. We reconstituted this intermembrane signaling geometry between live EphA2-expressing human breast cancer cells and supported membranes displaying laterally mobile ephrin-A1. Receptor-ligand binding, ... More