Search
Citations & References (7)
Invitrogen™
SNARF™-4F 5-(and-6)-Carboxylic Acid
細胞不透過性レシオメトリックpHインジケーターSNARF™-4F 5-（および-6）-カルボン酸（約6.4のpKa）は、酸性および塩基性条件下で、それぞれ黄橙色から深紅色へとpH依存的に有意な発光シフトを示します。このpH依存性により、2つの発光波長詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| S23920 | 1 mg |
製品番号（カタログ番号） S23920
価格（JPY）お問い合わせください ›
87,100
Each
数量:
1 mg
細胞不透過性レシオメトリックpHインジケーターSNARF™-4F 5-（および-6）-カルボン酸（約6.4のpKa）は、酸性および塩基性条件下で、それぞれ黄橙色から深紅色へとpH依存的に有意な発光シフトを示します。このpH依存性により、2つの発光波長（通常580 nmおよび640 nm）における色素からの蛍光強度比をpHの定量測定に使用できます。
カルシウム、カリウム、pH、膜電位インジケータなどのイオンインジケータの詳細をご覧ください›
カルシウム、カリウム、pH、膜電位インジケータなどのイオンインジケータの詳細をご覧ください›
研究用にのみ使用できます。診断用には使用いただけません。
仕様
検出法蛍光
染色剤タイプ蛍光色素ベース
インジケーターpHインジケータ
数量1 mg
出荷条件室温
使用対象 (装置)蛍光顕微鏡, フローサイトメーター, マイクロプレートリーダー
製品ラインSNARF
製品タイプpHインジケータ
Unit SizeEach
組成および保存条件
室温で保存し、光から保護します。
引用および参考文献 (7)
引用および参考文献
Abstract
Application of a pH-sensitive fluoroprobe (C-SNARF-4) for pH microenvironment analysis in Pseudomonas aeruginosa biofilms.
Journal:Appl Environ Microbiol
PubMed ID:15870340
'An important feature of microbial biofilms is the development of four-dimensional physical and chemical gradients in space and time. There is need for novel approaches to probe these so-called microenvironments to determine their effect on biofilm-specific processes. In this study, we describe the use of seminaphthorhodafluor-4F 5-(and-6) carboxylic acid (C-SNARF-4)
Characterization of dual-wavelength seminaphthofluorescein and seminapthorhodafluor dyes for pH sensing under high hydrostatic pressures.
Journal:Anal Biochem
PubMed ID:17274941
'Hydrostatic pressure is an important physical parameter in biology, with pressures in the few-hundred-atm range having significant effects on cellular morphology, metabolism, and viability. To ensure valid results when studying pressure effects using fluorescence spectroscopy and imaging methods, metabolic probes need to be characterized for high-pressure use. Of interest is
Fluorescent indicators for intracellular pH.
Journal:Chem Rev
PubMed ID:19831417
This review is about intracellular pH sensors, includingsmall fluorescent organic molecules, nanoparticles, andfluorescent proteins, e.g., GFP. It focuses on their preparations, photophysical properties, and advantages/disadvantagesfor intracellular pH measurements. The discussion is limitedto fluorescent indicators that have been applied to measureintracellular pH values since 1980.
Synthesis and photophysical properties of new fluorinated benzo[c]xanthene dyes as intracellular pH indicators.
Journal:Bioorg Med Chem Lett
PubMed ID:11677123
Two new fluorinated benzo[c]xanthene dyes were synthesized by reaction of fluorinated 1,6-dihydroxynaphthalenes with 2,4- (and 2,5)-dicarboxy-3'-dimethylamino-2'-hydroxybenzophenone. The two critical fluorinated 1,6-dihydroxynaphthalene intermediates were prepared via a regioselective route. The fluorinated benzo[c]xanthene dyes exhibit desired lower pK(a) values (6.4 and 7.2, respectively) than their parent compound (pK(a)=7.5) while the pH-dependent dual-emission
Single cell kinetics of intracellular, nonviral, nucleic acid delivery vehicle acidification and trafficking.
Journal:Bioconjug Chem
PubMed ID:16029041
Mechanistic understanding of the intracellular trafficking of nonviral nucleic acid delivery vehicles remains elusive. A live, single cell-based assay is described here that is used to investigate and quantitate the spatiotemporal, intracellular pH microenvironment of polymeric-based nucleic acid delivery vehicles. Polycations such as polyethylenimine (PEI), poly-l-lysine (PLL), beta-cyclodextrin-containing polymers lacking