SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor™ 680

Citations & References (5)

Invitrogen™

SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor™ 680

Alexa Fluor™ 680 SAIVI™抗体ラベリングキットは、6倍の抗体濃度範囲でin vivoイメージングアプリケーション（DOL；約2）に最適なレベルの標識を抗体に付ける便利な手段を提供します。反応量、色素濃度詳細を見る

製品番号（カタログ番号）	数量
S30045	1 kit

製品番号（カタログ番号） S30045

価格（JPY）

190,400

お問い合わせください ›

1 kit

Alexa Fluor™ 680 SAIVI™抗体ラベリングキットは、6倍の抗体濃度範囲でin vivoイメージングアプリケーション（DOL；約2）に最適なレベルの標識を抗体に付ける便利な手段を提供します。反応量、色素濃度、または抗体濃度を調整する必要はありません。この手順を使用すると、最適に標識された抗体を、生細胞イメージングや動物への直接注入など、アジ化物が含まれていない試薬を必要とするアプリケーションに使用できます。

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光

励起／発光679／702

標識タイプAlexa Fluor色素

標識スケール0.5～3 mg

製品ラインAlexa Fluor、SAIVI

製品タイプAntibody Labeling Kit

数量1 kit

出荷条件室温

Labeling Target抗体

標識または色素Alexa Fluor 680

Unit SizeEach

組成および保存条件

冷蔵庫（2℃～8℃）に保存し、遮光してください。

よくあるご質問（FAQ）

What amount of conjugated antibody should I inject to image tumors?

A recommended starting dosage is 50 µg. You will need to determine the optimal dosage for your experimental model.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What size needle should I use for small animal in vivo imaging?

We recommend the use of a 28-32 gauge tuberculin or insulin syringe (0.3 or 1.0 mL volume) with a fixed (non-removable) needle.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the optimal and maximal volumes of reagent that can be injected into a mouse?

The volume of reagent that can be injected varies according to the route of administration. The following numbers are general guidelines for a 25 gram animal: Intravenous (IV)- 50-125 µl (recommended)- 200µl (maximum) ; Intraperitoneal (IP) 500µl (recommended) -2ml (maximum) ; Subcutaneous(SC) 100-250 µl (recommended)- 1ml (maximum).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of mice are the best for in vivo imaging?

Due to light scattering caused by fur, hairless mice such as athymic nude (nu/nu) mice are recommended for in vivo imaging. If this is not an option, the hair covering the area to be imaged should be removed using clippers or a chemical depilatory such as Nair depilatory.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (5)

引用および参考文献

Abstract

Affibody Molecules for In vivo Characterization of HER2-Positive Tumors by Near-Infrared Imaging.

Authors:Lee SB, Hassan M, Fisher R, Chertov O, Chernomordik V, Kramer-Marek G, Gandjbakhche A, Capala J,

Journal:Clin Cancer Res

PubMed ID:18559604

'PURPOSE: HER2 overexpression has been associated with a poor prognosis and resistance to therapy in breast cancer patients. We are developing molecular probes for in vivo quantitative imaging of HER2 receptors using near-infrared (NIR) optical imaging. The goal is to provide probes that will minimally interfere with the studied system, ... More

Noninvasive positron emission tomography and fluorescence imaging of CD133+ tumor stem cells.

Authors:Gaedicke S, Braun F, Prasad S, Machein M, Firat E, Hettich M, Gudihal R, Zhu X, Klingner K, Schüler J, Herold-Mende CC, Grosu AL, Behe M, Weber W, Mäcke H, Niedermann G,

Journal:

PubMed ID:24469819

'A technology that visualizes tumor stem cells with clinically relevant tracers could have a broad impact on cancer diagnosis and treatment. The AC133 epitope of CD133 currently is one of the best-characterized tumor stem cell markers for many intra- and extracranial tumor entities. Here we demonstrate the successful noninvasive detection ... More

Minimally invasive quantification of lymph flow in mice and rats by imaging depot clearance of near-infrared albumin.

Authors:Karlsen TV, McCormack E, Mujic M, Tenstad O, Wiig H,

Journal:Am J Physiol Heart Circ Physiol

PubMed ID:22101523

'There is a lack of available methods to noninvasively quantify lymphatic function in small experimental animals, a necessity for studies on lymphatic system pathophysiology. We present a new method to quantify lymph flow in mice and rats, based on optically monitoring the depot clearance of near-infrared fluorescently labeled albumin and ... More

Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia.

Authors:McCormack E, Mujic M, Osdal T, Bruserud Ø, Gjertsen BT,

Journal:Blood

PubMed ID:23243270

Antibodies play a fundamental role in diagnostic immunophenotyping of leukemias and in cell-targeting therapy. However, this versatility is not reflected in imaging diagnostics. In the present study, we labeled anti–human mAbs monochromatically against selected human myeloid markers expressed on acute myeloid leukemia (AML) cells, all with the same near-infrared fluorochrome. ... More

Influenza virus aerosol exposure and analytical system for ferrets.

Authors:Gustin KM, Belser JA, Wadford DA, Pearce MB, Katz JM, Tumpey TM, Maines TR,

Journal:Proc Natl Acad Sci U S A

PubMed ID:21536880

Understanding the transmission ability of newly emerging influenza viruses is central to the development of public health preparedness and prevention strategies. Animals are used to model influenza virus infection and transmission, but the routinely used intranasal inoculation of a liquid virus suspension does not reflect natural infection. We report the ... More