SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor™ 750

Citations & References (3)

Invitrogen™

SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor™ 750

Alexa Fluor™ 750 SAIVI™抗体ラベリングキットは、6倍の抗体濃度範囲でin vivoイメージングアプリケーション（DOL；約2）に最適なレベルの標識を抗体に付ける便利な手段を提供します。反応量、色素濃度詳細を見る

製品番号（カタログ番号）	数量
S30046	1 Kit

製品番号（カタログ番号） S30046

価格（JPY）

190,400

お問い合わせください ›

1 Kit

Alexa Fluor™ 750 SAIVI™抗体ラベリングキットは、6倍の抗体濃度範囲でin vivoイメージングアプリケーション（DOL；約2）に最適なレベルの標識を抗体に付ける便利な手段を提供します。反応量、色素濃度、または抗体濃度を調整する必要はありません。この手順を使用すると、最適に標識された抗体を、生細胞イメージングや動物への直接注入など、アジ化物が含まれていない試薬を必要とするアプリケーションに使用できます。

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光

励起／発光749／775

標識タイプAlexa Fluor色素

標識スケール0.5～3 mg

製品ラインAlexa Fluor、SAIVI

製品タイプAntibody Labeling Kit

数量1 Kit

出荷条件室温

Labeling Target抗体

標識または色素Alexa Fluor 750

Unit SizeEach

組成および保存条件

冷蔵庫（2℃～8℃）に保存し、遮光してください。

よくあるご質問（FAQ）

What amount of conjugated antibody should I inject to image tumors?

A recommended starting dosage is 50 µg. You will need to determine the optimal dosage for your experimental model.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What size needle should I use for small animal in vivo imaging?

We recommend the use of a 28-32 gauge tuberculin or insulin syringe (0.3 or 1.0 mL volume) with a fixed (non-removable) needle.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the optimal and maximal volumes of reagent that can be injected into a mouse?

The volume of reagent that can be injected varies according to the route of administration. The following numbers are general guidelines for a 25 gram animal: Intravenous (IV)- 50-125 µl (recommended)- 200µl (maximum) ; Intraperitoneal (IP) 500µl (recommended) -2ml (maximum) ; Subcutaneous(SC) 100-250 µl (recommended)- 1ml (maximum).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of mice are the best for in vivo imaging?

Due to light scattering caused by fur, hairless mice such as athymic nude (nu/nu) mice are recommended for in vivo imaging. If this is not an option, the hair covering the area to be imaged should be removed using clippers or a chemical depilatory such as Nair depilatory.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (3)

引用および参考文献

Abstract

Affibody Molecules for In vivo Characterization of HER2-Positive Tumors by Near-Infrared Imaging.

Authors:Lee SB, Hassan M, Fisher R, Chertov O, Chernomordik V, Kramer-Marek G, Gandjbakhche A, Capala J,

Journal:Clin Cancer Res

PubMed ID:18559604

'PURPOSE: HER2 overexpression has been associated with a poor prognosis and resistance to therapy in breast cancer patients. We are developing molecular probes for in vivo quantitative imaging of HER2 receptors using near-infrared (NIR) optical imaging. The goal is to provide probes that will minimally interfere with the studied system, ... More

A leukocyte-mimetic magnetic resonance imaging contrast agent homes rapidly to activated endothelium and tracks with atherosclerotic lesion macrophage content.

Authors:McAteer MA, Mankia K, Ruparelia N, Jefferson A, Nugent HB, Stork LA, Channon KM, Schneider JE, Choudhury RP,

Journal:Arterioscler Thromb Vasc Biol

PubMed ID:22499989

Endothelial cell activation is an important mediator of monocyte recruitment to sites of vascular inflammation. We hypothesized that high-affinity dual-ligand microparticles of iron oxide (MPIO), targeted to P-selectin and vascular cell adhesion molecule-1 (PV-MPIO), would identify activated endothelial cells during atherosclerosis progression. In vivo magnetic resonance imaging in apolipoprotein E-deficient ... More

Blocking ephrinB2 with highly specific antibodies inhibits angiogenesis, lymphangiogenesis, and tumor growth.

Authors:Abéngozar MA, de Frutos S, Ferreiro S, Soriano J, Perez-Martinez M, Olmeda D, Marenchino M, Cañamero M, Ortega S, Megias D, Rodriguez A, Martínez-Torrecuadrada JL,

Journal:Blood

PubMed ID:22446484

Membrane-anchored ephrinB2 and its receptor EphB4 are involved in the formation of blood and lymphatic vessels in normal and pathologic conditions. Eph/ephrin activation requires cell-cell interactions and leads to bidirectional signaling pathways in both ligand- and receptor-expressing cells. To investigate the functional consequences of blocking ephrinB2 activity, 2 highly specific ... More