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Streptavidin, Alexa Fluor™ 405 conjugate
Streptavidin, Alexa Fluor™ 405 conjugate
Citations & References (4)
Invitrogen™

Streptavidin, Alexa Fluor™ 405 conjugate

当社のストレプトアビジン、Alexa Fluor 405コンジュゲート は、402 nmおよび421 nmの最大励起／発光波長を持つ明るく光安定性の高いプローブです。ストレプトアビジンは、細菌から単離され詳細を見る
テクニカルサポートオーダーサポート
製品番号（カタログ番号）数量
S323511 mg
製品番号（カタログ番号） S32351
価格（JPY）
67,300
Each
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数量:
1 mg
当社のストレプトアビジン、Alexa Fluor 405コンジュゲート は、402 nmおよび421 nmの最大励起／発光波長を持つ明るく光安定性の高いプローブです。ストレプトアビジンは、細菌から単離され、中性に近い等電点を持つ非グリコシル化ビオチン結合タンパク質です。グリコシル化されたビオチン結合タンパク質であるアビジンよりも非特異的結合が少ないと報告されているため、ビオチン化プローブの検出に広く使用されています。

すべてのAlexa Fluor™ストレプトアビジンおよびストレプトアビジンコンジュゲートの選択ガイドをご覧ください。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
検出法蛍光
励起／発光402/421
フローサイトメーターレーザーライン405
使用対象 (装置)フローサイトメトリー、免疫測定法、組織化学アプリケーション、ブロット分析
標識または色素Alexa Fluor 405
製品タイプストレプトアビジンコンジュゲート(蛍光)
数量1 mg
出荷条件室温
コンジュゲートAlexa Fluor 405
形状固体
製品ラインAlexa Fluor
Unit SizeEach
組成および保存条件
Streptavidin Alexa Fluor™ 405 conjugateのバイアル1本を含みます。

フリーザー(-5∼-30度)に保存し、遮光してください。

よくあるご質問（FAQ）

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

引用および参考文献 (4)

引用および参考文献
Abstract
Violet laser diodes in flow cytometry: an update.
Authors:Telford W, Kapoor V, Jackson J, Burgess W, Buller G, Hawley T, Hawley R,
Journal:Cytometry A
PubMed ID:17051581
INTRODUCTION: In previous studies we and others have demonstrated the usefulness of violet laser diodes (VLDs) as replacement laser sources for krypton-ion lasers on stream-in-air cytometers. Previously available VLDs had a maximum available power of less than 25 mW; this was sufficient for excitation of densely labeled cell surface antigens ... More
Novel strategy to selectively label excitatory and inhibitory neurons in the cerebral cortex of mice.
Authors:Basu K, Gravel C, Tomioka R, Kaneko T, Tamamaki N, Sík A,
Journal:J Neurosci Methods
PubMed ID:18321591
Revealing the connections of neuronal systems is critical for understanding how they function. The vast majority of neurons in all cortical areas consist of excitatory cells whose activity is controlled by inhibitory cells. Distribution and projection patterns of inhibitory and excitatory cells are key information to understand the organization of ... More
Endosomal trafficking of the ligated FcvarepsilonRI receptor.
Authors:Fattakhova GV, Masilamani M, Narayanan S, Borrego F, Gilfillan AM, Metcalfe DD, Coligan JE,
Journal:Mol Immunol
PubMed ID:18945491
In addition to initiating signaling cascades leading to mast cell mediator release, aggregation of the high affinity IgE receptor (FcvarepsilonRI) leads to rapid internalization of the cross-linked receptor. However, little is known about the trafficking of the internalized FcvarepsilonRI. Here we demonstrate that in RBL-2H3 cells, aggregated FcvarepsilonRI appears in ... More
Inducible gene targeting in the neonatal vasculature and analysis of retinal angiogenesis in mice.
Authors:Pitulescu ME, Schmidt I, Benedito R, Adams RH,
Journal:Nat Protoc
PubMed ID:20725067
The retina is a powerful experimental system for the analysis of angiogenic blood vessel growth in the postnatal organisms. The three-dimensional architecture of the vessel network and processes as diverse as endothelial cell (EC) proliferation, sprouting, perivascular cell recruitment, vessel remodeling or maturation can be investigated at high resolution. The ... More