SelectFX™ Nuclear Labeling Kit (DAPI, SYTOX™ Green, 7-AAD, TO-PRO™-3 Iodide), for fixed cells

Citations & References (14)

Invitrogen™

SelectFX™ Nuclear Labeling Kit (DAPI, SYTOX™ Green, 7-AAD, TO-PRO™-3 Iodide), for fixed cells

SelectFX™核ラべリングキット™™は、固定細胞調製における核の染色用に、4種類のスペクトルが異なる蛍光色素を提供します。青色蛍光DAPI、緑色蛍光SYTOXグリーン染色、赤色蛍光7-アミノアクチノマイシンD（7-AAD）、遠赤色蛍光TO-PRO3色素です。これらの色素は詳細を見る

製品番号（カタログ番号）	数量
S33025	1 kit

製品番号（カタログ番号） S33025

価格（JPY）

44,800

お問い合わせください ›

1 kit

SelectFX™核ラべリングキット™™は、固定細胞調製における核の染色用に、4種類のスペクトルが異なる蛍光色素を提供します。青色蛍光DAPI、緑色蛍光SYTOXグリーン染色、赤色蛍光7-アミノアクチノマイシンD（7-AAD）、遠赤色蛍光TO-PRO3色素です。これらの色素は、マルチカラーアプリケーションでの対比染色に最適です。サンプルに適用される他の蛍光プローブとスペクトル的に対照的な染色剤を選択するだけです。SelectFX™核ラべリングキットの色素は、提供されたプロトコルに従って使用すると、細胞質標識をほとんどまたはまったく行うことなく、高選択性核染色を実現します。

研究用にのみ使用できます。診断用には使用いただけません。

色Green

検出法蛍光

染色剤タイプ細胞非透過性

使用対象 (装置)蛍光顕微鏡、フローサイトメーター

製品ラインSELECTFX, SYTOX, TO-PRO

数量1 kit

出荷条件室温

標識タイプFluorescent Dye

製品タイプNuclear Labeling Kit

SubCellular Localization核、核酸, Nucleus

Unit SizeEach

組成および保存条件

フリーザー（-5℃～-30℃）に保存し、遮光してください。

よくあるご質問（FAQ）

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

My DAPI labeled samples have a strong blue background signal immediately after mounting. What can I do to fix this?

Some mounting medias can have strong blue autofluorescence. If you are seeing a high blue background, it could be coming from the mountant. Try labeling the sample and view it before (using a wet mount in buffer) and after mounting to determine if the background signal is coming from the mounting media or the sample itself.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am using SYTOX AAdvanced as a dead cell stain, but all of my cells are labeling even though I am certain that they are supposed to be alive. These are adherent cells that I have trypsinized. Why am I getting false-dead signals?

SYTOX AAdvanced labels only dead cells because it is a cell impermeant dye. The dye can only enter cells that have a compromised plasma membrane. Trypsinization may cause temporary disruption of the plasma membrane, sufficient to allow staining with a cell impermeant dye. You can reduce the “false-dead” problem by either reducing the amount of trypsin and/or reduce the incubation time for trypsinization or use a gentler dissociation reagent such as TrypLE Express, TrypLESelect reagents, or Versene. After trypsinization, wash well, and if possible, allow a recovery time in normal culture media before staining with any of the SYTOX dyes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (14)

引用および参考文献

Abstract

Avoiding cytotoxicity of transposases by dose-controlled mRNA delivery.

Authors:Galla M, Schambach A, Falk CS, Maetzig T, Kuehle J, Lange K, Zychlinski D, Heinz N, Brugman MH, Göhring G, Izsvák Z, Ivics Z, Baum C,

Journal:Nucleic Acids Res

PubMed ID:21609958

'The Sleeping Beauty (SB) transposase and its newly developed hyperactive variant, SB100X, are of increasing interest for genome modification in experimental models and gene therapy. The potential cytotoxicity of transposases requires careful assessment, considering that residual integration events of transposase expression vectors delivered by physicochemical transfection or episomal retroviral vectors ... More

Protein transduction from retroviral Gag precursors.

Authors:Voelkel C, Galla M, Maetzig T, Warlich E, Kuehle J, Zychlinski D, Bode J, Cantz T, Schambach A, Baum C,

Journal:Proc Natl Acad Sci U S A

PubMed ID:20385817

Retroviral particles assemble a few thousand units of the Gag polyproteins. Proteolytic cleavage mediated by the retroviral protease forms the bioactive retroviral protein subunits before cell entry. We hypothesized that this process could be exploited for targeted, transient, and dose-controlled transduction of nonretroviral proteins into cultured cells. We demonstrate that ... More

Insulin-like growth factor-1 sustains stem cell mediated renal repair.

Authors:Imberti B, Morigi M, Tomasoni S, Rota C, Corna D, Longaretti L, Rottoli D, Valsecchi F, Benigni A, Wang J, Abbate M, Zoja C, Remuzzi G,

Journal:J Am Soc Nephrol

PubMed ID:17942965

In mice with cisplatin-induced acute kidney injury, administration of bone marrow-derived mesenchymal stem cells (MSC) restores renal tubular structure and improves renal function, but the underlying mechanism is unclear. Here, we examined the process of kidney cell repair in co-culture experiments with MSC and cisplatin-injured proximal tubular epithelial cells (PTEC). ... More

Dual probe with fluorescent and magnetic properties for imaging solid tumor xenografts.

Authors:Shan L, Wang S, Sridhar R, Bhujwalla ZM, Wang PC

Journal:Mol Imaging

PubMed ID:17445503

A dual probe with fluorescent and magnetic reporter groups was constructed by linkage of the near-infrared (NIR) fluorescent transferrin conjugate (Tf(NIR)) on the surface of contrast agent-encapsulated cationic liposome (Lip-CA). This probe was used for magnetic resonance imaging (MRI) and optical imaging of MDA-MB-231-luc breast cancer cells grown as a ... More

Using total internal reflection fluorescence microscopy to visualize rhodopsin-containing cells.

Authors:

Journal:Appl Environ Microbiol

PubMed ID:25769822

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