SYBR™ Green II RNA Gel Stain, 10,000X concentrate in DMSO

Citations & References (25)

Invitrogen™

SYBR™ Green II RNA Gel Stain, 10,000X concentrate in DMSO

Name: SYBR™ Green II RNA Gel Stain, 10,000X concentrate in DMSO
SKU: S7568

SYBR Green II RNAゲル染色剤は、RNAに結合すると明るい蛍光を示します。ゲルのバックグラウンドが低い高感度核酸ゲル染色剤で、レーザースキャナーまたは標準的なUVトランスイルミネーターを使用したホルムアルデヒド／アガロースゲルまたはポリアクリルアミドゲルでの使用に最適です。500 µLのユニットサイズ（S-7564詳細を見る

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製品番号（カタログ番号）	数量
S7568	1 mL
S7564	500 μL
S7586	20x50 μL

3 オプション

製品番号（カタログ番号） S7568

価格（JPY）

165,600

Each

お問い合わせください ›

数量:

1 mL

一括またはカスタム形式をリクエストする

SYBR Green II RNAゲル染色剤は、RNAに結合すると明るい蛍光を示します。ゲルのバックグラウンドが低い高感度核酸ゲル染色剤で、レーザースキャナーまたは標準的なUVトランスイルミネーターを使用したホルムアルデヒド／アガロースゲルまたはポリアクリルアミドゲルでの使用に最適です。500 µLのユニットサイズ（S-7564）と1 mLのユニットサイズ（S-7568）をご用意しています。

研究用にのみ使用できます。診断用には使用いただけません。

仕様

検出位置ゲル内検出

検出法蛍光

製品タイプRNAゲル染色

数量1 mL

出荷条件室温

標的分子RNA

標識または色素SYBRグリーンII

Unit SizeEach

組成および保存条件

DMSOで10,000倍濃縮液として提供されます。遮光し、乾燥器内で-20℃で保存します

よくあるご質問（FAQ）

What is the pH range of SYBR dyes?

The SYBR dyes are useful only over a narrow range of pH, from about 7 to 8. Outside this range, the fluorescent signal diminishes rapidly.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the recommended filter for my gel documentation system?

Please go here (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-gel-electrophoresis/dna-stains/sybr-safe.html) and click on the “Filter Recommendations” tab to see filter recommendations for use with SYBR Safe DNA Gel Stain. Note that the excitation and emission spectra of SYBR Safe DNA Gel Stain are very similar to those of SYBR Green I, SYBR Green II, and SYBR Gold dyes, as well as fluorescein (FITC). Therefore, filters appropriate for these dyes can also be used. A camera filter is not required with the Safe Imager Blue-Light Transilluminator; the amber filter provided with the instrument serves this purpose.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Can I use the ethidium bromide filters on my camera to image SYBR dyes?

This is not recommended. Most deep amber/orange ethidium bromide filters have a cutoff value around 550 nm. The SYBR Green dyes emit at 520 nm, which will not be detected using this filter.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Is SYBR Safe DNA Gel Stain the same as SYBR Green I dye?

All SYBR dyes have similar spectral properties, but have different chemical compositions. All SYBR dyes bind to dsDNA, ssDNA and RNA but vary in sensitivity. SYBR Safe DNA Gel Stain (Cat. No. S33102) was specifically developed as a safer alternative to ethidium bromide. SYBR Green I (Cat. No. S7585) is an ultrasensitive stain for dsDNA, and SYBR Green II (Cat. No. S7564) is a highly sensitive stain for RNA and ssDNA. All SYBR dyes are optimally excited by the Safe Imager Blue-Light Transilluminator.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What are the differences between all the SYBR dyes?

Different SYBR dyes bind to dsDNA, ssDNA, and RNA, but vary in the sensitivity and specificity with which they bind to different nucleic acids. SYBR Green I Nucleic Acid Gel Stain is used for staining dsDNA and ssDNA. SYBR Green II RNA Gel Stain will stain dsDNA and ssDNA but has better sensitivity for RNA. SYBR Gold Acid Gel Stain was developed after SYBR Green I and II and is the most sensitive fluorescent gel stain offering the highest sensitivity for both DNA and RNA. SYBR Safe DNA Gel Stain is a reduced mutagenicity formula designed for use with blue light systems. It is less sensitive than the SYBR Green I and II but comparable to ethidium bromide.

Please check the following link for additional details regarding the different SYBR dyes: The Molecular Probes Handbook: Nucleic Acid Detection on Gels, Blots and Arrays

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

View all SYBR™ Green II RNA Gel Stain, 10,000X concentrate in DMSO, 1 mL FAQs

引用および参考文献 (25)

引用および参考文献

Abstract

Authors:

Journal:

PubMed ID:8660526

SPACRCAN, a novel human interphotoreceptor matrix hyaluronan-binding proteoglycan synthesized by photoreceptors and pinealocytes.

Authors:Acharya S, Foletta VC, Lee JW, Rayborn ME, Rodriguez IR, Young WS, Hollyfield JG

Journal:J Biol Chem

PubMed ID:10702256

The interphotoreceptor matrix is a unique extracellular complex occupying the interface between photoreceptors and the retinal pigment epithelium in the fundus of the eye. Because of the putative supportive role in photoreceptor maintenance, it is likely that constituent molecules play key roles in photoreceptor function and may be targets for ... More

Lateral-flow and up-converting phosphor reporters to detect single-stranded nucleic acids in a sandwich-hybridization assay.

Authors:Corstjens PL, Zuiderwijk M, Nilsson M, Feindt H, Sam Niedbala R, Tanke HJ

Journal:Anal Biochem

PubMed ID:12531205

'Up-converting Phosphor Technology (UPT) particles were used as reporters in lateral-flow (LF) assays to detect single-stranded nucleic acids. The 400-nm phosphor particles exhibit strong visible luminescence upon excitation with infrared (IR) light resulting in the total absence of background autofluorescence from other biological compounds. A sandwich-type hybridization assay was applied ... More

Naturally occurring mutations in human mitochondrial pre-tRNASer(UCN) can affect the transfer ribonuclease Z cleavage site, processing kinetics, and substrate secondary structure.

Authors:Yan H, Zareen N, Levinger L

Journal:J Biol Chem

PubMed ID:16361254

'tRNAs are transcribed as precursors with a 5'' end leader and a 3'' end trailer. The 5'' end leader is processed by RNase P, and in most organisms in all three kingdoms, transfer ribonuclease (tRNase) Z can endonucleolytically remove the 3'' end trailer. Long ((L)) and short ((S)) forms of ... More

Protein-free parallel triple-stranded DNA complex formation.

Authors:Shchyolkina AK, Timofeev EN, Lysov YP, Florentiev VL, Jovin TM, Arndt-Jovin DJ

Journal:Nucleic Acids Res

PubMed ID:11160932

'A 14 nt DNA sequence 5''-AGAATGTGGCAAAG-3'' from the zinc finger repeat of the human KRAB zinc finger protein gene ZNF91 bearing the intercalator 2-methoxy,6-chloro,9-amino acridine (Acr) attached to the sugar-phosphate backbone in various positions has been shown to form a specific triple helix (triplex) with a 16 bp hairpin (intramolecular) ... More

View all SYBR™ Green II RNA Gel Stain, 10,000X concentrate in DMSO, 1 mL citations