iBind™ Western Starter Kit, fluorescent detection
iBind™ Western Starter Kit, fluorescent detection
Invitrogen™

iBind™ Western Starter Kit, fluorescent detection

蛍光検出用のiBind Western Starter Kitには、iBind Western Device、iBindカード、iBind蛍光検出溶液キットが含まれているため、自動化されたブロット処理をすぐに開始できます詳細を見る
製品番号(カタログ番号)数量
SLF1002S1 kit
製品番号(カタログ番号) SLF1002S
価格(JPY)
258,000
1 kit
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数量:
1 kit
蛍光検出用のiBind Western Starter Kitには、iBind Western Device、iBindカード、iBind蛍光検出溶液キットが含まれているため、自動化されたブロット処理をすぐに開始できます。

iBind Western Deviceは、ウェスタンブロット処理(免疫検出)を自動化する、小型の卓上用デバイスです。iBindカードに機械的圧力をかけることで、ブロッキング、抗体結合、および洗浄ステップ用の免疫検出溶液のシーケンシャルラテラルフロー(SLF)を生成します。iBindカードのガラス繊維マトリックスにより、溶液が絶えず均一にメンブレン上を流れるため、抗原と抗体の相互作用が促進されます。

iBind Western Deviceを使用すると、手順の開始時にすべての溶液が調製されてデバイスにローディングされます。SLFの動作により、以降のすべてのステップは電気やバッテリーなしで自動的に続行されます。ブロットは、約2.5時間で最終検出ステップで使用可能になります。

iBind Western Systemsの詳細 ›

iBind Western DeviceはiBindカードiBind溶液キットまたはiBind蛍光検出(FD)溶液キット(1ミニブロットを処理する場合)でのみ機能します。

研究用にのみ使用できます。診断用には使用いただけません。
仕様
数量1 kit
出荷条件室温
容量1×ミニブロット
検出法蛍光、化学発光、比色分析
使用対象(アプリケーション)Western Blot
Membrane Compatibilityニトロセルロース、PVDF
製品ラインiBind
Running Time∼2.5 hr.
スループット1 Mini Blot
タイプスターターキット
Unit Size1 kit
組成および保存条件
1個のiBindウェスタン装置、10枚のiBindカード(室温で保存)、1個のiBind蛍光検出(FD)ソリューションキット(2~8℃で保存)

よくあるご質問(FAQ)

Do you offer additional years of service for the iBind Western Device?

No, we do not provide service contracts or extended warranties for these devices. The warranty is limited to 1 year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What procedure should I follow when stripping and reprobing my blot processed using the iBind Western Device or iBind Flex Western Device?

To strip and reprobe your blot processed using the iBind Western Device or iBind Flex Western Device, first strip the blot outside the system using your preferred protocol. After the stripping protocol, wash the blot in distilled water for 5 min. Reblocking is unnecessary as the iBind Solution will perform this step during reprobing. Finally, reprobe with new antibodies using the iBind Western Device or iBind Flex Western Device, starting with the membrane preparation step.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I multiplex antibodies using the iBind Western Device or iBind Flex Western Device?

Yes, for each midi blot, mini blot, or vertically cut strip, prepare appropriate primary antibodies together in a single tube of iBind FD/ iBind Flex FD Solution, Prepare appropriate secondary antibodies together in a different tube of iBind FD/ iBind Flex FD Solution. Do not mix primary and secondary antibodies prior to loading in the device. All primary and secondary antibodies should be diluted according to the recommendations outlined in the user manual or quick reference card. When detecting several targets of the same species, the same concentration of secondary antibody can be used as when detecting one target. There is no need to increase the concentration for each additional target. Load multiplexed antibodies into the device as normal.
Caution: Consider cross-reactivity of secondary antibodies when multiplexing (e.g., rabbit anti-goat IgG and goat anti-mouse IgG are likely to cross-react).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the iBind Western Device or iBind Flex Western Device with Alexa Fluor Plus conjugated secondary antibodies?

Yes, both the iBind Western Device and iBind Flex Western Device work well with Alexa Fluor Plus conjugated secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

With the iBind Western Device and iBind Flex Western Device, how do I know if I am rolling the membrane firmly enough?

When firmly rolling, the iBind/iBind Flex Card will slightly dip in the membrane area, causing the top and bottom of the card to lift. See the iBind/iBind Flex user manual for additional details.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.