NuPAGE™ Midi Tris-Acetate Resupply Pack, 3 to 8%, WedgeWell™ format

The NuPAGE Tris-Acetate Wedgewell Resupply Pack simplifies your large molecular weight protein separation workflow. This pack, sufficient for 100 gel runs, includes precast midi gels, running buffer, and protein ladders. NuPAGE Tris-Acetate gels offer high-resolution protein separation while maintaining protein integrity. Designed for optimal separation and transfer they are ideal for researchers investigating large molecular weight proteins. With a long shelf life this pack is a reliable and convenient solution for your laboratory needs.

Resupply Pack contents

• NuPAGE Tris-Acetate WedgeWell gels (10 boxes, 100 gels)
• NuPAGE Tris-Acetate Running Buffer for 100 gel runs, 20X (5 bottles, Cat. No. LA0041)
• HiMark Pre-stained Protein Standard for 100 gel runs, 30 to 460 kDa (4 vials, Cat. No. LC5699)

About NuPAGE Tris-Acetate gels

Invitrogen NuPAGE Tris-Acetate protein gels are precast polyacrylamide gels specifically engineered to provide excellent separation of large molecular weight proteins. These gels do not contain SDS, enabling you to run your proteins in native or denatured form, offering maximum flexibility for diverse protein electrophoresis needs. The unique buffer formulation of these gels maintains a low operating pH during electrophoresis, resulting in superior resolution of proteins when compared to traditional Tris-glycine SDS-PAGE gels.

Not only do NuPAGE Tris-Acetate gels offer high-resolution separation, but they are also designed to ensure optimal transfer of larger molecular weight proteins, making them a preferred choice for researchers performing western blotting. These gels represent a significant advancement over traditional gels, providing both superior performance and flexibility for protein gel electrophoresis and transfer. Additionally, midi format gels offer increased sample throughput per gel, enhancing efficiency, increasing reproducibility, and decreasing cost per sample.

Separation

For running Invitrogen protein gels, you have the flexibility to use either the SureLock Tandem Midi Gel Tank (STM1001) or the XCell4 SureLock Midi-Cell (WR0100). Both systems are designed to provide optimal performance. The SureLock Tandem offers rapid and efficient electrophoresis of midi gels in a leak-free system, with minimal buffer usage. The two independent chambers allow for electrophoresis of one or two gels simultaneously, conserving buffer and reducing waste.

Alternatively, the XCell4 SureLock Midi-Cell is a robust and efficient system for midi-gel electrophoresis. Its standout feature is its capacity to simultaneously run up to four midi gels, enabling high-throughput protein analysis with enhanced efficiency and convenience. Additionally, the SureLock Tandem Midi Gel Tank is conveniently available through Invitrogen Precast Gel Welcome Packs along with both sample buffer and reducing agent, providing an all-in-one solution for your electrophoresis needs.

Transfer

For transferring your proteins to a membrane, we recommend using for NuPAGE Transfer Buffer (NP0006) for traditional wet transfer using the SureLock Tandem Midi Blot Module (STM2001). Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 3 Gel Transfer Device (IB31001).

Convenient to use

NuPAGE Tris-Acetate WedgeWell-format gels have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0-mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.