Search
Citations & References (319)
Invitrogen™
FM™ 4-64 Dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide)
スチリル色素FM 4-64は、酵母の真空膜を赤色蛍光（最大励起／最大発光は約515/640 nm）で選択的に染色することが報告されています。この親油性色素は、真空オルガネラの形態とダイナミクスを可視化し、エンドサイトーシス経路を研究し、酵母エンドサイトーシス変異体のスクリーニングと特性評価を行うための重要なツールです詳細を見る
製品番号（カタログ番号） T13320
価格（JPY）お問い合わせください ›
129,500
10 x 100 µg
数量:
10 x 100 μg
スチリル色素FM 4-64は、酵母の真空膜を赤色蛍光（最大励起／最大発光は約515/640 nm）で選択的に染色することが報告されています。この親油性色素は、真空オルガネラの形態とダイナミクスを可視化し、エンドサイトーシス経路を研究し、酵母エンドサイトーシス変異体のスクリーニングと特性評価を行うための重要なツールです。FM 4-64は、それぞれ100 µgのバイアル10個を含む特殊パッケージ（T-13320）でも使用できます。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
色Red
検出法蛍光
使用対象 (装置)蛍光顕微鏡
製品ラインFM
数量10 x 100 μg
出荷条件室温
標識タイプFluorescent Dye
製品タイプ色素
SubCellular Localization細胞膜
Unit Size10 x 100 µg
組成および保存条件
室温で保存し、光から保護します。
よくあるご質問（FAQ）
I want to study endosomes trafficking using FM 4-64. Will the label be retained after fixation? And can I label already-fixed cells?
引用および参考文献 (319)
引用および参考文献
Abstract
Apg9p/Cvt7p is an integral membrane protein required for transport vesicle formation in the Cvt and autophagy pathways.
Journal:The Journal of cell biology
PubMed ID:10662773
In nutrient-rich, vegetative conditions, the yeast Saccharomyces cerevisiae transports a resident protease, aminopeptidase I (API), to the vacuole by the cytoplasm to vacuole targeting (Cvt) pathway, thus contributing to the degradative capacity of this organelle. When cells subsequently encounter starvation conditions, the machinery that recruited precursor API (prAPI) also sequesters
Glutamate induces the rapid formation of spine head protrusions in hippocampal slice cultures.
Journal:Proceedings of the National Academy of Sciences of the United States of America
PubMed ID:15831587
Synaptic plasticity at neuronal connections has been well characterized functionally by using electrophysiological approaches, but the structural basis for this phenomenon remains controversial. We have studied the dynamic interactions between presynaptic and postsynaptic structures labeled with FM 4-64 and a membrane-targeted GFP, respectively, in hippocampal slices. Under conditions of reduced
Fab1p is essential for PtdIns(3)P 5-kinase activity and the maintenance of vacuolar size and membrane homeostasis.
Journal:The Journal of cell biology
PubMed ID:9763421
The Saccharomyces cerevisiae FAB1 gene encodes a 257-kD protein that contains a cysteine-rich RING-FYVE domain at its NH2-terminus and a kinase domain at its COOH terminus. Based on its sequence, Fab1p was initially proposed to function as a phosphatidylinositol 4-phosphate (PtdIns(4)P) 5-kinase (). Additional sequence analysis of the Fab1p kinase
Slow spontaneous secretion from single large dense-core vesicles monitored in neuroendocrine cells.
Journal:FASEB J
PubMed ID:15180959
Hormones are released from cells by passing through an exocytotic pore that forms after vesicle and plasma membrane fusion. In stimulated exocytosis vesicle content is discharged swiftly. Although rapid vesicle discharge has also been proposed to mediate basal secretion, this has not been studied directly. We investigated basal hormone release
Traffic-independent function of the Sar1p/COPII machinery in proteasomal sorting of the cystic fibrosis transmembrane conductance regulator.
Journal:J Cell Biol
PubMed ID:12538638
'Newly synthesized proteins that do not fold correctly in the ER are targeted for ER-associated protein degradation (ERAD) through distinct sorting mechanisms; soluble ERAD substrates require ER-Golgi transport and retrieval for degradation, whereas transmembrane ERAD substrates are retained in the ER. Retained transmembrane proteins are often sequestered into specialized ER