FM™ 5-95 (N-(3-Trimethylammoniumpropyl)-4-(6-(4-(Diethylamino)phenyl)hexatrienyl)Pyridinium Dibromide)

FM™ 5-95 (<i>N</i>-(3-Trimethylammoniumpropyl)-4-(6-(4-(Diethylamino)phenyl)hexatrienyl)Pyridinium Dibromide)

Citations & References (14)

Invitrogen™

FM™ 5-95 (N-(3-Trimethylammoniumpropyl)-4-(6-(4-(Diethylamino)phenyl)hexatrienyl)Pyridinium Dibromide)

FM色素のアプリケーションの成功数が増えるにつれ、本質的に同じ分光特性を持つFM 4-64（T-3166、T-13320）のわずかに親油性の低い類似体である、FM5-95を合成するようになりました。詳細を見る

製品番号（カタログ番号）	数量
T23360	1 mg

製品番号（カタログ番号） T23360

価格（JPY）

114,000

お問い合わせください ›

FM色素のアプリケーションの成功数が増えるにつれ、本質的に同じ分光特性を持つFM 4-64（T-3166、T-13320）のわずかに親油性の低い類似体である、FM5-95を合成するようになりました。

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光

使用対象 (装置)蛍光顕微鏡

製品ラインFM

数量1 mg

出荷条件室温

標識タイプFluorescent Dye

製品タイプ色素

SubCellular Localization細胞膜

Unit Size1 mg

組成および保存条件

室温で保存し、光から保護します。

よくあるご質問（FAQ）

I want to study endosome trafficking using FM 4-64 dye. Will the label be retained after fixation? And can I label cells that have already been fixed?

No. For that, you would need the FM 4-64FX version. The non-FX version will be lost, leading to loss of much of the specific label and a vast increase in background labeling. The FX version will be fixed in place with formaldehyde. Cells that have been fixed already will not label correctly, so you will need to label the cells live and then fix.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (14)

引用および参考文献

Abstract

Glutamate receptor dynamics organizing synapse formation in vivo.

Authors:Rasse TM, Fouquet W, Schmid A, Kittel RJ, Mertel S, Sigrist CB, Schmidt M, Guzman A, Merino C, Qin G, Quentin C, Madeo FF, Heckmann M, Sigrist SJ

Journal:Nat Neurosci

PubMed ID:16136672

'Insight into how glutamatergic synapses form in vivo is important for understanding developmental and experience-triggered changes of excitatory circuits. Here, we imaged postsynaptic densities (PSDs) expressing a functional, GFP-tagged glutamate receptor subunit (GluR-IIA(GFP)) at neuromuscular junctions of Drosophila melanogaster larvae for several days in vivo. New PSDs, associated with functional ... More

Fluorescence ratio imaging microscopy shows decreased access of vancomycin to cell wall synthetic sites in vancomycin-resistant Staphylococcus aureus.

Authors:Pereira PM, Filipe SR, Tomasz A, Pinho MG,

Journal:Antimicrob Agents Chemother

PubMed ID:17646417

'A new method of fluorescence ratio imaging microscopy was used to compare the in vivo binding capacity and the access of a fluorescent derivative of vancomycin to the cell wall synthetic sites in isogenic pairs of vancomycin-susceptible and -resistant laboratory mutants and vancomycin-intermediate and -susceptible clinical isolates of Staphylococcus aureus. ... More

Single synaptic vesicle tracking in individual hippocampal boutons at rest and during synaptic activity.

Authors:Lemke EA, Klingauf J

Journal:J Neurosci

PubMed ID:16306416

'How synaptic vesicles move within central nervous synapses to their docking sites at the plasma membrane is widely discussed in synaptic physiology. This question is especially difficult to investigate in the small hippocampal boutons, which themselves can slowly move during observation in primary cell culture. Here, we describe a single ... More

SynCAM, a synaptic adhesion molecule that drives synapse assembly.

Authors:Biederer T, Sara Y, Mozhayeva M, Atasoy D, Liu X, Kavalali ET, Südhof TC

Journal:Science

PubMed ID:12202822

'Synapses, the junctions between nerve cells through which they communicate, are formed by the coordinated assembly and tight attachment of pre- and postsynaptic specializations. We now show that SynCAM is a brain-specific, immunoglobulin domain-containing protein that binds to intracellular PDZ-domain proteins and functions as a homophilic cell adhesion molecule at ... More

Lipid spirals in Bacillus subtilis and their role in cell division.

Authors:Barák I, Muchová K, Wilkinson AJ, O'Toole PJ, Pavlendová N,

Journal:Mol Microbiol

PubMed ID:18430139

The fluid mosaic model of membrane structure has been revised in recent years as it has become evident that domains of different lipid composition are present in eukaryotic and prokaryotic cells. Using membrane binding fluorescent dyes, we demonstrate the presence of lipid spirals extending along the long axis of cells ... More

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