CLIPR-59, a new trans-Golgi/TGN cytoplasmic linker protein belonging to the CLIP-170 family.
Authors:Perez F, Pernet-Gallay K, Nizak C, Goodson HV, Kreis TE, Goud B
Journal:J Cell Biol
PubMed ID:11854307
'The microtubule cytoskeleton plays a fundamental role in cell organization and membrane traffic in higher eukaryotes. It is well established that molecular motors are involved in membrane-microtubule interactions, but it has also been proposed that nonmotor microtubule-binding (MTB) proteins known as CLIPs (cytoplasmic linker proteins) have basic roles in these ... More
Geldanamycin stimulates internalization of ErbB2 in a proteasome-dependent way.
Authors:Lerdrup M, Hommelgaard AM, Grandal M, van Deurs B
Journal:J Cell Sci
PubMed ID:16352662
'The potent oncoprotein and receptor tyrosine kinase ErbB2 is remarkable because it resists efficient downregulation. However, ErbB2 can be downregulated by the HSP-90 inhibitor geldanamycin, but the underlying cellular mechanisms are uncertain. Apparently, delivery of ErbB2 to lysosomes, cleavage of the ErbB2 kinase domain and proteasomal activity are all processes ... More
Evaluation of disulfide reduction during receptor-mediated endocytosis by using FRET imaging.
Authors:Yang J, Chen H, Vlahov IR, Cheng JX, Low PS
Journal:Proc Natl Acad Sci U S A
PubMed ID:16950881
'Despite functional evidence for disulfide bond-reducing activity in endosomal compartments, the mechanistic details pertaining to such process (e.g., kinetics and sites of disulfide reduction) remain largely controversial. To address these questions directly, we have synthesized a previously uncharacterized fluorescent folate conjugate, folate-(BODIPY FL)-SS-rhodamine (folate-FRET), that changes fluorescence from red to ... More
Self-assembled, aptamer-tethered DNA nanotrains for targeted transport of molecular drugs in cancer theranostics.
Authors:Zhu G, Zheng J, Song E, Donovan M, Zhang K, Liu C, Tan W,
Journal:Proc Natl Acad Sci U S A
PubMed ID:23630258
'Nanotechnology has allowed the construction of various nanostructures for applications, including biomedicine. However, a simple target-specific, economical, and biocompatible drug delivery platform with high maximum tolerated doses is still in demand. Here, we report aptamer-tethered DNA nanotrains (aptNTrs) as carriers for targeted drug transport in cancer therapy. Long aptNTrs were ... More
Pincher, a pinocytic chaperone for nerve growth factor/TrkA signaling endosomes.
Authors:Shao Y, Akmentin W, Toledo-Aral JJ, Rosenbaum J, Valdez G, Cabot JB, Hilbush BS, Halegoua S
Journal:J Cell Biol
PubMed ID:12011113
A central tenet of nerve growth factor (NGF) action that is poorly understood is its ability to mediate cytoplasmic signaling, through its receptor TrkA, that is initiated at the nerve terminal and conveyed to the soma. We identified an NGF-induced protein that we termed Pincher (pinocytic chaperone) that mediates endocytosis ... More