JC-1 Dye (Mitochondrial Membrane Potential Probe)

Citations & References (400)

Invitrogen™

JC-1 Dye (Mitochondrial Membrane Potential Probe)

JC-1は、新規の陽イオン性カルボシアニン色素で、ミトコンドリアに蓄積します。この色素は低濃度で単量体として存在し、蛍光色素と同様に緑色蛍光を発します。高濃度では、この色素はJ凝集体を形成し、広範囲の励起スペクトルと最大発光約590 nmを示します。これらの特性により詳細を見る

製品番号（カタログ番号）	数量
T3168	5 mg

製品番号（カタログ番号） T3168

価格（JPY）

90,600

온라인 행사

Ends: 27-Mar-2026

129,500

割引額 38,900 (30%)

お問い合わせください ›

JC-1は、新規の陽イオン性カルボシアニン色素で、ミトコンドリアに蓄積します。この色素は低濃度で単量体として存在し、蛍光色素と同様に緑色蛍光を発します。高濃度では、この色素はJ凝集体を形成し、広範囲の励起スペクトルと最大発光約590 nmを示します。これらの特性により、JC-1はミトコンドリア膜電位の高感度マーカーとなっています。他にも、同等の特性を持つ色素がJC-9（D- 22421）です。

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光

数量5 mg

出荷条件室温

細胞内局在ミトコンドリア

色Green

使用対象 (装置)蛍光顕微鏡, フローサイトメーター

製品タイプ色素

Unit Size5 mg

組成および保存条件

室温で保存し、光から保護します。

よくあるご質問（FAQ）

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between fast and slow-response membrane potential probes?

Molecules that change their structure in response to the surrounding electric field can function as fast-response probes for the detection of transient (millisecond) potential changes. Slow-response dyes function by entering depolarized cells and binding to proteins or membranes. Increased depolarization results in additional dye influx and an increase in fluorescence, while hyperpolarization is indicated by a decrease in fluorescence. Fast-response probes are commonly used to image electrical activity from intact heart tissues or measure membrane potential changes in response to pharmacological stimuli. Slow-responding probes are often used to explore mitochondrial function and cell viability.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of membrane potential indicators do you offer and how should I choose one for my experiment?

A membrane potential indicator selection guide can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (400)

引用および参考文献

Abstract

Apoptosis induced by Rac GTPase correlates with induction of FasL and ceramides production.

Authors:Embade N,Valerón PF,Aznar S,López-Collazo E,Lacal JC

Journal:Molecular biology of the cell

PubMed ID:11102528

Rho proteins, members of the Ras superfamily of GTPases, are critical elements in signal transduction pathways governing cell proliferation and cell death. Different members of the family of human Rho GTPases, including RhoA, RhoC, and Rac1, participate in the regulation of apoptosis in response to cytokines and serum deprivation in ... More

Large-scale chemical dissection of mitochondrial function.

Authors:Wagner BK,Kitami T,Gilbert TJ,Peck D,Ramanathan A,Schreiber SL,Golub TR,Mootha VK

Journal:Nature biotechnology

PubMed ID:18297058

Mitochondrial oxidative phosphorylation (OXPHOS) is central to physiology and disease pathogenesis. To systematically investigate its activity and regulation, we performed a wide range of assays of OXPHOS physiology and nuclear and mitochondrial gene expression across 2490 chemical perturbations in muscle cells. Through mining of the resulting compendium, we discovered that: ... More

Authors:

Journal:

PubMed ID:10891486

Mitochondria--potential role in cell life and death.

Authors:Griffiths EJ

Journal:Cardiovascular research

PubMed ID:10727650

The mitochondrial death/life regulator in apoptosis and necrosis.

Authors:Kroemer G,Dallaporta B,Resche-Rigon M

Journal:Annual review of physiology

PubMed ID:9558479

Both physiological cell death (apoptosis) and, in some cases, accidental cell death (necrosis) involve a two-step process. At a first level, numerous physiological and some pathological stimuli trigger an increase in mitochondrial membrane permeability. The mitochondria release apoptogenic factors through the outer membrane and dissipate the electrochemical gradient of the ... More

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