Tubulin Tracker™ Green (Oregon Green™ 488 Taxol, bis-acetate)
Tubulin Tracker™ Green (Oregon Green™ 488 Taxol, bis-acetate)
Citations & References (9)
Invitrogen™

Tubulin Tracker™ Green (Oregon Green™ 488 Taxol, bis-acetate)

Tubulin Tracker Green（Oregon Green 488 Taxol、 bis-acetate）は詳細を見る
テクニカルサポートオーダーサポート
製品番号（カタログ番号）数量
T34078スライド60枚
製品番号（カタログ番号） T34078
価格（JPY）
24,800
60 slides
お問い合わせください ›
数量:
スライド60枚
Tubulin Tracker Green（Oregon Green 488 Taxol、 bis-acetate）は、生細胞中の重合チューブリンの緑色蛍光染色を行います。Tubulin Tracker Greenは帯電していない非蛍光化合物で、生細胞の細胞膜を容易に通過します。細胞内に入ると、親油性ブロッキング基は非特異的なエステラーゼによって切断され、緑色蛍光の荷電型を形成し、生細胞の流出を防止します。TubulinTracker Green試薬は、Oregon Green 488色素を使用しています。この色素はそれぞれ494 nmと522 nmで最適に吸収および発光し、蛍光はほぼすべての蛍光イメージング装置を使用して標準の GFP/FITCフィルター設定で可視化できます。青、オレンジ、赤、濃赤、近赤のフルオロフォアでマルチプレックスできます。
研究用途にのみご使用ください。診断目的には使用できません。
仕様
染色剤タイプOregon Green™ 488
励起波長域494/522
製品ラインチューブリントラッカー
数量スライド60枚
出荷条件氷水またはドライアイスでの出荷が承認されています
標識タイプUV-Vis標識
製品タイプTubulin Tracker
SubCellular Localizationチューブリン
Unit Size60 slides
組成および保存条件
1スライドあたり1 mLで60枚以上のスライドおよび等倍の作業濃度に十分な材料が含まれています。

1 x 20 μg Tubulin Tracker Green(Oregon Green 488 Taxol、bis-アセテート)

1 x 1 mL Pluronic F-127、DMSO中に20%の溶液

よくあるご質問（FAQ）

What is the difference between Tubulin Tracker Green (Cat No. T34078) and Tubulin Tracker Green, for live cell imaging (Cat. No. T34075)?

Both products include the same Tubulin Tracker Green reagent. The difference is in the packaging and amount of Tubulin Tracker Green reagent contained. Tubulin Tracker Green, for live cell imaging (Cat. No. T34075) includes enough Tubulin Tracker Green reagent for 300 slides whereas Tubulin Tracker Green (Cat No. T34078) contains enough Tubulin Tracker Green reagent for 60 slides. Also, Tubulin Tracker Green, for live cell imaging (Cat. No. T34075) does not include Probenecid.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

With Tubulin Tracker Deep Red and Tubulin Tracker Green, can I fix samples after labeling?

No. The labeling is not retained upon fixation. Imaging of the samples should be done only on live cells. To limit photobleaching while viewing live cells, use ProLong Live Antifade Reagent, for live cell imaging (Cat No. P36974 and P36975).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is Docetaxel in Tubulin Tracker Deep Red different from Taxol in Tubulin Tracker Green? Are there any differences in the binding or cytotoxicity between the two reagents?

Docetaxel has a slightly higher binding affinity than Taxol and exhibits longer retention time due to better uptake and slower efflux, but for use in labeling cultured cells, these differences should be minimal. More information comparing the properties of these two reagents is provided in this publication: https://www.ncbi.nlm.nih.gov/pubmed/1671606 https://www.cell.com/fulltext/S1074-5521(05)00301-7

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (9)

引用および参考文献
Abstract
Involvement of 14-3-3 in tubulin instability and impaired axon development is mediated by Tau.
Authors:Joo Y, Schumacher B, Landrieu I, Bartel M, Smet-Nocca C, Jang A, Choi HS, Jeon NL, Chang KA, Kim HS, Ottmann C, Suh YH,
Journal:
PubMed ID:26103986
'14-3-3 proteins act as adapters that exert their function by interacting with their various protein partners. 14-3-3 proteins have been implicated in a variety of human diseases including neurodegenerative diseases. 14-3-3 proteins have recently been reported to be abundant in the neurofibrillary tangles (NFTs) observed inside the neurons of brains ... More
Impact of C24:0 on actin-microtubule interaction in human neuronal SK-N-BE cells: evaluation by FRET confocal spectral imaging microscopy after dual staining with rhodamine-phalloidin and tubulin tracker green.
Authors:Zarrouk A, Nury T, Dauphin A, Frère P, Riedinger JM, Bachelet CM, Frouin F, Moreau T, Hammami M, Kahn E, Lizard G,
Journal:
PubMed ID:26214025
'Disorganization of the cytoskeleton of neurons has major consequences on the transport of neurotransmitters via the microtubule network. The interaction of cytoskeleton proteins (actin and tubulin) was studied in neuronal SK-N-BE cells treated with tetracosanoic acid (C24:0), which is cytotoxic and increased in Alzheimer''s disease patients. When SK-N-BE cells were ... More
The Ovary of Tubifex tubifex (Clitellata, Naididae, Tubificinae) Is Composed of One, Huge Germ-Line Cyst that Is Enriched with Cytoskeletal Components.
Authors:Urbisz AZ, Chajec L, Swiatek P,
Journal:
PubMed ID:26001069
Recent studies on the ovary organization and oogenesis in Tubificinae have revealed that their ovaries are small polarized structures that are composed of germ cells in subsequent stages of oogenesis that are associated with somatic cells. In syncytial cysts, as a rule, each germ cell is connected to the central ... More
Multimodal two-photon imaging using a second harmonic generation-specific dye.
Authors:Nuriya M, Fukushima S, Momotake A, Shinotsuka T, Yasui M, Arai T,
Journal:Nat Commun
PubMed ID:27156702
'Second harmonic generation (SHG) imaging can be used to visualize unique biological phenomena, but currently available dyes limit its application owing to the strong fluorescent signals that they generate together with SHG. Here we report the first non-fluorescent and membrane potential-sensitive SHG-active organic dye Ap3. Ap3 is photostable and generates ... More
Tubulin is a molecular target of the Wnt-activating chemical probe.
Authors:Fukuda Y, Sano O, Kazetani K, Yamamoto K, Iwata H, Matsui J,
Journal:BMC Biochem
PubMed ID:27207629
In drug discovery research, cell-based phenotypic screening is an essential method for obtaining potential drug candidates. Revealing the mechanism of action is a key step on the path to drug discovery. However, elucidating the target molecules of hit compounds from phenotypic screening campaigns remains a difficult and troublesome process. Simple ... More
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