What is the difference between Tubulin Tracker Green (Cat No. T34078) and Tubulin Tracker Green, for live cell imaging (Cat. No. T34075)?
Both products include the same Tubulin Tracker Green reagent. The difference is in the packaging and amount of Tubulin Tracker Green reagent contained. Tubulin Tracker Green, for live cell imaging (Cat. No. T34075) includes enough Tubulin Tracker Green reagent for 300 slides whereas Tubulin Tracker Green (Cat No. T34078) contains enough Tubulin Tracker Green reagent for 60 slides. Also, Tubulin Tracker Green, for live cell imaging (Cat. No. T34075) does not include Probenecid.
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With Tubulin Tracker Deep Red and Tubulin Tracker Green, can I fix samples after labeling?
No. The labeling is not retained upon fixation. Imaging of the samples should be done only on live cells. To limit photobleaching while viewing live cells, use ProLong Live Antifade Reagent, for live cell imaging (Cat No. P36974 and P36975).
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How is Docetaxel in Tubulin Tracker Deep Red different from Taxol in Tubulin Tracker Green? Are there any differences in the binding or cytotoxicity between the two reagents?
Docetaxel has a slightly higher binding affinity than Taxol and exhibits longer retention time due to better uptake and slower efflux, but for use in labeling cultured cells, these differences should be minimal. More information comparing the properties of these two reagents is provided in this publication: https://www.ncbi.nlm.nih.gov/pubmed/1671606 https://www.cell.com/fulltext/S1074-5521(05)00301-7
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Is Tubulin Tracker Deep Red acetylated? Does it require intracellular esterase activity to make the reagent active?
Tubulin Tracker Deep Red is not acetylated. It is inherently cell permeable and is not modified further by any cellular enzymatic activity to fluoresce or bind to actin.
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Do I need to use Probenecid when using Tubulin Tracker Deep Red?
The use of Probenecid is optional. Its requirement is dependent upon the cell type. Some cell types actively efflux the reagent out of the cells within a short time, limiting detection, other cell types do not exhibit this activity or exhibit very limited activity.
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