Thiol and Sulfide Quantitation Kit

チオールおよびスルフィド定量キットは、タンパク質および非タンパク質チオール（スルフヒドリルまたはメルカプタンとも呼ばれる）の両方を定量するための、超高感度の比色分析を提供します。このアッセイでは、チオールまたは無機硫化物はジスルフィド結合を阻害されたパパイン誘導体を還元し、化学量論的に活性酵素を放出します。次に、色素産生性のパパイン基質であるN-ベンゾイル-L-アルギニン詳細を見る

製品番号（カタログ番号）	数量
T6060	1 Kit

製品番号（カタログ番号） T6060

価格（JPY）

69,900

1 kit

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1 Kit

チオールおよびスルフィド定量キットは、タンパク質および非タンパク質チオール（スルフヒドリルまたはメルカプタンとも呼ばれる）の両方を定量するための、超高感度の比色分析を提供します。このアッセイでは、チオールまたは無機硫化物はジスルフィド結合を阻害されたパパイン誘導体を還元し、化学量論的に活性酵素を放出します。次に、色素産生性のパパイン基質であるN-ベンゾイル-L-アルギニン、p-ニトロアニリンド（L-BAPNA）を使用して、この酵素の活性を測定します。このキットでは、酵素増幅ステップにより、わずか0.2 µMのチオールを検出できます。これは、エルマン試薬を使用した場合の約100倍の感度です。

研究用にのみ使用できます。診断用には使用いただけません。

数量1 Kit

製品タイプThiol and Sulfide Quantitation Kit

Unit Size1 kit

引用および参考文献 (15)

引用および参考文献

Abstract

Site-directed fluorescence studies of a prokaryotic ClC antiporter.

Authors:Bell SP, Curran PK, Choi S, Mindell JA

Journal:Biochemistry

PubMed ID:16734414

'Channels and transporters of the ClC family serve a variety of physiological functions. Understanding of their gating and transport mechanisms remains incomplete, with disagreement over the extent of protein conformational change involved. Using site-directed fluorescence labeling, we probe ClC-ec1, a prokaryotic ClC, for transport-related structural rearrangements. We specifically label cysteines ... More

Reversible glutathionylation of complex I increases mitochondrial superoxide formation.

Authors:Taylor ER, Hurrell F, Shannon RJ, Lin TK, Hirst J, Murphy MP

Journal:J Biol Chem

PubMed ID:12649289

'Increased production of reactive oxygen species (ROS) by mitochondria is involved in oxidative damage to the organelle and in committing cells to apoptosis or senescence, but the mechanisms of this increase are unknown. Here we show that ROS production by mitochondrial complex I increases in response to oxidation of the ... More

Fullerene water suspension (nC60) exerts antibacterial effects via ROS-independent protein oxidation.

Authors:Lyon DY, Alvarez PJ,

Journal:Environ Sci Technol

PubMed ID:19031913

Buckminsterfullerene (C60) can form water suspensions (nC60) that exert toxic effects. While reactive oxygen species (ROS) generation has been implicated as the mechanism for mammalian cytotoxicity, we propose that nC60 exerts ROS-independent oxidative stress in bacteria, with evidence of protein oxidation, changes in cell membrane potential, and interruption of cellular ... More

A sensitive assay for maleimide groups.

Authors:Singh R

Journal:Bioconjug Chem

PubMed ID:7948101

A sensitive spectrophotometric assay has been developed for maleimide groups incorporated into proteins. The assay involves the reaction of maleimide with an excess of cysteine and quantitation of the remaining cysteine using an enzymatic assay that is based on the stoichiometric conversion of an inactive disulfide form of papain to ... More

An amplified assay for thiols based on reactivation of papain.

Authors:Singh R, Blättler WA, Collinson AR

Journal:Anal Biochem

PubMed ID:8238881

A sensitive spectrophotometric assay has been developed for thiol (sulfhydryl) groups using an inactive disulfide derivative of papain (papain-S-SCH3). The thiol-disulfide interchange reaction of a thiol with papain-S-SCH3 results in the stoichiometric formation of active papain (papain-SH). The reactivated papain catalyzes the hydrolysis of a chromogenic substrate, resulting in an ... More

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