Texas Red™ Hydrazide, >90% single isomer
Texas Red™ Hydrazide, >90% single isomer
Invitrogen™

Texas Red™ Hydrazide, >90% single isomer

赤色蛍光のTexas Red™ヒドラジドの一次脂肪族アミンは、アルデヒドおよびケトンと可逆的に結合してシッフ塩基を形成できます。シッフ塩基は、水素化ホウ素ナトリウム(NaBH4)またはシアノ水素化ホウ素ナトリウム(NaCNH3)によって安定したアミン誘導体に還元して、新しいビオチン化プローブを形成できます詳細を見る
製品番号(カタログ番号)数量
T62565 mg
製品番号(カタログ番号) T6256
価格(JPY)
62,600
5 mg
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数量:
5 mg
赤色蛍光のTexas Red™ヒドラジドの一次脂肪族アミンは、アルデヒドおよびケトンと可逆的に結合してシッフ塩基を形成できます。シッフ塩基は、水素化ホウ素ナトリウム(NaBH4)またはシアノ水素化ホウ素ナトリウム(NaCNH3)によって安定したアミン誘導体に還元して、新しいビオチン化プローブを形成できます。タンパク質や他の水溶性バイオポリマーのカルボン酸は、EDAC(E2247)などの水溶性カルボジイミドを使用して水溶液中のこの分子に結合できます。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
化学反応性カルボン酸、ケトン、アルデヒド
標識または色素Texas Red™
製品タイプヒドラジド
数量5 mg
反応性部分アミン,ヒドラジド
出荷条件室温
標識タイプ従来の色素
製品ラインTexas Red
Unit Size5 mg
組成および保存条件
フリーザー(-5∼-30度)に保存し、遮光してください。

引用および参考文献 (6)

引用および参考文献
Abstract
Exogenous glycosaminoglycans coat damaged bladder surfaces in experimentally damaged mouse bladder.
Authors:Kyker KD, Coffman J, Hurst RE,
Journal:BMC Urol
PubMed ID:15788101
'BACKGROUND: Interstital cystitis is often treated with exogenous glycosaminoglycans such as heparin, chondroitin sulphate (Uracyst), hyaluronate (Cystistat) or the semi-synthetic pentosan polysulphate (Elmiron). The mechanism of action is presumed to be due to a coating of the bladder surface to replace the normally present chondroitin sulphate and heparan sulphate lost ... More
Determination of progesterone and 17-hydroxyprogesterone by high performance liquid chromatography after pre-column derivatization with 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionohydra zide.
Authors:Katayama M, Nakane R, Matsuda Y, Kaneko S, Hara I, Sato H
Journal:Analyst
PubMed ID:10396811
'Progesterone, 17-hydroxyprogesterone and four other 3-keto steroids were determined by high performance liquid chromatography with fluorescence detection. Each steroid was derivatized with 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene++ +-3- propionohydrazide (BODIPY FL hydrazide) and separated on a Wakosil 5C4 column with acetonitrile-water (7 + 3) as mobile phase. The limits of detection of progesterone, 17-hydroxyprogesterone, ... More
Mycobacterium and the coat of many lipids.
Authors:Russell DG, Mwandumba HC, Rhoades EE
Journal:J Cell Biol
PubMed ID:12147678
'Pathogenic Mycobacterium reside inside vacuoles in their host macrophages. These vacuoles fail to fuse with lysosomes yet interact with early endosomes. Glycoconjugates released by the intracellular bacilli traffic through the host cell and are released through exocytosis. These molecules represent both antigens for immune recognition and modulators of immune function. ... More
Optimization of oxidation of glycoproteins: an assay for predicting coupling to hydrazide chromatographic supports.
Authors:Morehead HW, Talmadge KW, O'Shannessy DJ, Siebert CJ
Journal:J Chromatogr
PubMed ID:1723733
A rapid, simple assay for aldehydes generated by oxidation of saccharide units in glycoproteins, using dyes containing hydrazide functionalities, is described. The assay is used, in conjunction with tests of biological activity, to predict oxidation conditions that will result in a maximum of active protein coupled to a hydrazide chromatographic ... More
Direct photoaffinity labeling of cysteine-295 of alpha-tubulin by guanosine 5'-triphosphate bound in the nonexchangeable site.
Authors:Bai R, Choe K, Ewell JB, Nguyen NY, Hamel E
Journal:J Biol Chem
PubMed ID:9545331
The alphabeta-tubulin heterodimer has two high affinity guanosine 5'-triphosphate binding sites, so that purified tubulin usually contains two molecules of bound guanosine nucleotide. Half this nucleotide is freely exchangeable with exogenous guanine nucleotide, and its binding site has been readily localized to the beta-subunit. The remaining nonexchangeable guanosine 5'-triphosphate can ... More